Ribonuclease A

Native Bovine Ribonuclease A

Product Type
Purified Protein
Specificity
Ribonuclease A

Product Code Applications Pack Size List Price Your Price Qty
8040-0106
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Native ribonuclease A is purified RNase A isolated from bovine pancreas. RNase A is an endoribonuclease that exists as a single chain polypeptide containing 4 disulphide bridges. It can be inhibited by alkylation of the histidine-12 or histidine-119 that are present in the active site. Activators of RNase A include potassium and sodium salts.

The optimal conditions for activity are 60ºC and pH 7.6.

CAS number: 9001-99-4
EC number: 3.1.27.5

Target Species
Bovine
Product Form
Purified Protein - lyophilized
Reconstitution
Reconstitute to a concentration of 1.0 mg/ml with distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preservative Stabilisers
None present
Protein Molecular Weight
13.7 kDa
Regulatory
For research purposes only
Guarantee
6 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Synonyms
RNASE1
UniProt
P61823
Entrez Gene
RNASE1
GO Terms
GO:0005515 protein binding
GO:0003676 nucleic acid binding
GO:0004522 pancreatic ribonuclease activity
GO:0005576 extracellular region
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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