Ribonuclease A

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Native Bovine Ribonuclease A

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
8040-0106 E FN 100 mg
Native ribonuclease A is purified RNase A isolated from bovine pancreas. RNase A is an endoribonuclease that exists as a single chain polypeptide containing 4 disulphide bridges. It can be inhibited by alkylation of the histidine-12 or histidine-119 that are present in the active site. Activators of RNase A include potassium and sodium salts.

The optimal conditions for activity are 60ÂșC and pH 7.6.

CAS number: 9001-99-4
EC number: 3.1.27.5

Product Details

Target Species
Bovine
Product Form
Purified Protein - lyophilised
Reconstitution
Reconstitute to a concentration of 1mg/ml with distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preservative Stabilisers
None present
Molecular Weight
13.7 kDa

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
6 months from date of reconstitution.

More Information

UniProt
P61823 Related reagents
Entrez Gene
RNASE1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003676 nucleic acid binding
GO:0004522 pancreatic ribonuclease activity
GO:0005576 extracellular region
Regulatory
For research purposes only

Applications of Ribonuclease A

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.