Leukemia Inhibitory Factor
Recombinant Bovine leukemia inhibitory factor (PBP036) detected using Chicken anti Bovine leukemia inhibitory factor (AHP2381) as the capture reagent and Chicken anti Bovine leukemia inhibitory factor:Biotin (AHP2381B) as the detection reagent followed by Streptavidin:HRP
Recombinant Bovine Leukemia Inhibitory Factor
- Product Type
- Recombinant Protein
- Specificity
- Leukemia Inhibitory Factor
Antibody Quality and Performance Guarantee
| Recombinant Bovine leukemia inhibitory factor (LIF) is a recombinant protein corresponding to aa 23-202 of native bovine LIF. Bovine LIF is a chemokine belonging to the IL-6 cytokine family. LIF inhibits differentiation in normal stem cells, but in leukemic myeloid cells, induces differential thereby preventing further growth. |
- Target Species
- Bovine
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Source
- Pichia pastoris
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 19.8 kDa
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Synonyms
- LIF
- UniProt
- Q27956
- Entrez Gene
- LIF
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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