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IL-17A

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Bovine IL-17A ELISA

Recombinant Bovine IL-17A (PBP025) detected using Rabbit anti Bovine IL-17A (AHP2376) as the capture reagent and Rabbit anti Bovine IL-17A:Biotin (AHP2376B) as the detection reagent followed by Streptavidin:HRP

Recombinant Bovine Interleukin-17A

Product Type
Recombinant Protein
Specificity
IL-17A

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Product Code Applications Pack Size List Price Your Price Qty
PBP025
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 5 µg loader
List Price Your Price
loader

Recombinant Bovine interleukin-17A is a recombinant protein corresponding to aa 22 - 153 of bovine interleukin-17A (IL-17A). IL-17A is a disulfide-linked homodimer and member of the IL-17 family, secreted primarily by activated CD4+ T cells, which signal through the IL-17 receptor (IL-17R).

IL-17 induces a variety of target cells to secrete inflammatory cytokines, including IL-1β, IL-6, TNFα, IFNγ and granulocyte colony-stimulating factor, as well as synergizing with other inflammatory cytokines and agonists.

Target Species
Bovine
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Source
Pichia pastoris
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
15.0 kDa
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2376 as a coating antibody and AHP2376B as a detection antibody.

UniProt
Q687Y7
Entrez Gene
IL17A
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006954 inflammatory response

PBP025

151725 167473

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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