IGF-I
Recombinant Bovine insulin-like growth factor I (PBP034) detected using Goat anti Bovine insulin-like growth factor I as the capture reagent and biotinylated Goat anti Bovine insulin-like growth factor I as the detection reagent followed by Streptavidin:HRP.
Recombinant Bovine Insulin-Like Growth Factor I
- Product Type
- Recombinant Protein
- Specificity
- IGF-I
Antibody Quality and Performance Guarantee
| Recombinant Bovine insulin-like growth factor I (IGF-I) is a recombinant protein corresponding to aa 50-119 of native bovine IGF-I. IGF-I is a secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver, which effects a wide range of cells and plays a key role in cell proliferation and growth, inhibition of apoptosis, and is linked with tumor cell growth. IGF-I signals by binding to the type 1 insulin-like growth factor receptor (IGF-IR) and also to the insulin receptor (IR), resulting in the activation of the AKT signaling pathway. IGF-I production is stimulated by growth hormone (GH) and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway. |
- Target Species
- Bovine
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Source
- Pichia pastoris
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 7.6 kDa
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Synonyms
- Somatomedin C
- UniProt
- P07455
- Entrez Gene
- IGF1
- GO Terms
- GO:0008083 growth factor activity
- GO:0046326 positive regulation of glucose import
- GO:0045725 positive regulation of glycogen biosynthetic process
View more products with IGF specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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