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IFN Alpha A

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Bovine Interferon alpha A ELISA

Recombinant Bovine interferon alpha A (PBP019) detected using Goat anti Bovine interferon alpha A (AHP2370) as the capture reagent and Goat anti Bovine interferon alpha A:Biotin (AHP2370B) as the detection reagent followed by Streptavidin:HRP

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Recombinant Bovine Interferon Alpha A

Product Type
Recombinant Protein
Specificity
IFN Alpha A

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Product Code Applications Pack Size List Price Your Price Qty
PBP019
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 5 µg loader
List Price Your Price
loader

Recombinant Bovine interferon alpha A (IFNαA) is a recombinant protein of approximately 19.0 kDa corresponding to aa 24-189 of native bovine IFNαA.

Bovine IFNαA is a type I interferon belonging to the alpha/beta interferon family. IFNαA is produced by macrophages and involved in defense against viral infections.

Target Species
Bovine
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Source
Pichia pastoris
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
19.0 kDa
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2370 as a coating antibody and AHP2370B as a detection antibody.

UniProt
P05007
Entrez Gene
IFNAA
GO Terms
GO:0006952 defense response
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005126 cytokine receptor binding
GO:0005615 extracellular space

PBP019

144659 156331 158309 160445

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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