Calmodulin
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Native Bovine Calmodulin
- Product Type
- Purified Protein
- Specificity
- Calmodulin
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| Calmodulin is a ubiquitous 16.6 kDa protein found in all eukaryote cells that is involved with the control of a large number of calcium sensitive enzymes. It can bind to and regulate a number of different protein targets, thereby affecting many different cellular functions. |
- Target Species
- Bovine
- Product Form
- Purified calmodulin from bovine brain - lyophilized
- Reconstitution
- Reconstitute to a concentration of 0.1 - 1.0 mg/ml in distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Preparation
- Purified by ion-exchange chromatography followed by gel-filtration
- Preservative Stabilisers
- 2 mM EDTA.
- Activity
- More than 40,000 units per 1 mg of protein.One unit will stimulate 0.016 activated units of phosphodiesterase, 3'5'-cyclic nucleotide, P 0520, to 50% of the maximum activity of the enzyme when saturated with activator.
- Purity
- > 95%. Determined by SDS-PAGE.
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- UniProt
- P62157
- Entrez Gene
- CALM
- GO Terms
- GO:0005515 protein binding
- GO:0000922 spindle pole
- GO:0005509 calcium ion binding
- GO:0005829 cytosol
- GO:0010880 regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
- GO:0060315 negative regulation of ryanodine-sensitive calcium-release channel activity
- GO:0060316 positive regulation of ryanodine-sensitive calcium-release channel activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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