Calmodulin

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Native Bovine Calmodulin

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
1740-2384 E 1 mg
Calmodulin is a ubiquitous 16.6 kDa protein found in all eukaryote cells that is involved with the control of a large number of calcium sensitive enzymes. It can bind to and regulate a number of different protein targets, thereby affecting many different cellular functions.

Product Details

Target Species
Bovine
Product Form
Purified calmodulin from bovine brain - lyophilised
Reconstitution
Reconstitute to a concentration of 0.1 - 1 mg/ml in distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial.
Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified by ion-exchange chromatography followed by gel-filtration.
Preservative Stabilisers
2 mM EDTA.
Activity
More than 40,000 units per 1 mg of protein.

One unit will stimulate 0.016 activated units of phosphodiesterase, 3'5'-cyclic nucleotide, P 0520, to 50% of the maximum activity of the enzyme when saturated with activator.
Purity
> 95%. Determined by SDS-PAGE.

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P62157 Related reagents
Entrez Gene
CALM Related reagents
GO Terms
GO:0005515 protein binding
GO:0000922 spindle pole
GO:0005509 calcium ion binding
GO:0005829 cytosol
GO:0010880 regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
GO:0060315 negative regulation of ryanodine-sensitive calcium-release channel activity
GO:0060316 positive regulation of ryanodine-sensitive calcium-release channel activity
Regulatory
For research purposes only

Applications of Calmodulin

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.