IgG2a antibody
Goat anti Mouse IgG2a:FITC
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG2a
Goat anti Mouse IgG2a antibody recognizes Mouse IgG2a. This antibody has been cross absorbed against mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Goat anti Mouse IgG2a antibody shows minimal cross-reactivity with human immunoglobulins. |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Antiserum Preparation
- Antisera to IgG2a were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- IgG2a paraproteins from BALB/c mice
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/1000 | ||
Immunofluorescence | Neat | 1/10 | |
Immunohistology - Frozen |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for IgG2a antibody
-
Knipping, K. et al. (2011) A gastrointestinal rotavirus infection mouse model for immune modulation studies.
Virol J. 8: 109. -
Bagai, U. & Pawar, A. (2013) A blood stage fraction of Plasmodium berghei. induces protective and long lasting immune response in BALB/c mice.
Parasitol Int. 62 (3): 329-36. -
Hwang, S.R. et al. (2015) Altered expression levels of neurodevelopmental proteins in fetal brains of BTBR T+tf/J mice with autism-like behavioral characteristics.
J Toxicol Environ Health A. 78 (8): 516-23. -
Zhao, Z. et al. (2015) Multiple B-cell epitope vaccine induces a Staphylococcus enterotoxin B-specific IgG1 protective response against MRSA infection.
Sci Rep. 5: 12371. -
Minaei, S. et al. (2018) Propranolol efficacy as a novel adjuvant for immunization against Toxoplasma gondii. tachyzoites.
Exp Parasitol. 194: 60-66. -
Kushwaha, V. et al. (2019) Troponin 1 of human filarial parasite Brugia malayi.: cDNA cloning, expression, purification, and its immunoprophylactic potential.
Parasitol Res. 118 (6): 1849-63. -
Nedumpun, T. et al. (2019) Negative Immunomodulatory Effects of Type 2 Porcine Reproductive and Respiratory Syndrome Virus-Induced Interleukin-1 Receptor Antagonist on Porcine Innate and Adaptive Immune Functions.
Front Immunol. 10: 579. -
Gatkowska, J. et al. (2019) The Impact of the Antigenic Composition of Chimeric Proteins on Their Immunoprotective Activity against Chronic Toxoplasmosis in Mice.
Vaccines (Basel). 7(4):154.
View The Latest Product References
-
Mola, S. et al. (2020) A transcriptome-based approach to identify functional modules within and across primary human immune cells.
PLoS One. 15 (5): e0233543. -
Han, H. et al. (2021) Metal arsenic mediated enhancement of type-2 immunity in brains with altered locomotive activities in mice with autism-like behavioral characteristics
Toxicological Research. 38 (1): 27-33. -
Sessevmez, M. et al. (2023) Induction of humoral and cell-mediated immunity in mice by chitosan-curdlan composite nanoparticles administered intranasally and subcutaneously
J Drug Deliv Sci Technol. 86: 104704. -
Bauer, L. et al. (2023) The pro-inflammatory response to influenza A virus infection is fueled by endothelial cells.
Life Sci Alliance. 6 (7): e202201837. -
Faber, E. et al. (2024) Identification of T cell and linear B cell epitopes on African horse sickness virus serotype 4 proteins VP1-1, VP2, VP4, VP7 and NS3.
Vaccine. 42 (2): 136-45. -
Kusma, S. et al. (2024) Oral subacute polypropylene microplastics administration effect on potential immunotoxicity in ICR mice.
J Toxicol Environ Health A. : 1-10 [Epub ahead of print].
- RRID
- AB_1102657
- UniProt
- P01865
- P01863
- P01864
- Entrez Gene
- Igh-1a
- Igh-1a
- Igh-1a
- GO Terms
- GO:0003823 antigen binding
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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