CD27 antibody | LT27
Mouse anti Human CD27:StarBright Red 715
- Product Type
- Monoclonal Antibody
- Clone
- LT27
- Isotype
- IgG2a
- Specificity
- CD27
Antibody Quality and Performance Guarantee
Figure B FITC conjugated Mouse anti Human CD19 antibody, clone LT19 (MCA1940F) and Alexa Fluor® 647 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755A647). All experiments performed on human peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Mouse anti Human CD27 antibody, clone LT27 (MCA755GA) used for the evaluation of CD27 expression on human B cell subsets by flow cytometry.
Image caption:
Memory B cell sorting strategies.
Subsets of human B cells (CD45+CD19+) were sorted from BM and spleens of vaccinated HIS mice. The strategy 1 (upper part) was used on 3 responder HBsAg-vaccinated animals and gave rise to the following B cell subsets: (i) CD27hiCD38hi, (ii) CD27+CD38lo/intIgD+, and (iii) CD27+CD38lo/intIgD−. The strategy 2 (lower part) was used on 3 responder TT-vaccinated animals and gave rise to the following B cell subsets: (iv) CD27+IgM+IgG−, and (v) CD27+IgM−IgG+. The respective frequency and name (from i to v) of each sorted B cell subsets is indicated in the corresponding flow cytometry dot plot.).
From: Becker PD, Legrand N, van Geelen CMM, Noerder M, Huntington ND, Lim A, et al. (2010)
Generation of Human Antigen-Specific Monoclonal IgM Antibodies Using Vaccinated “Human Immune System” Mice.
PLoS ONE 5(10): e13137.
doi: 10.1371/journal.pone.0013137.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Figure B. Alexa Fluor® 647 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463A647) and StarBright Violet 515 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Data acquired on the ZE5 Cell Analyzer.
RPE-Cy5 conjugate is no longer available. 46 alternative formats are available.
Figure B. RPE-Cy5 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463C*) and StarBright Violet 610 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBV610). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
*N.B. RPE-Cy5 conjugate is no longer available. 46 alternative formats are available.
Figure B. RPE-Cy5 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463C*) and StarBright Violet 610 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBV610). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
*N.B. RPE-Cy5 conjugate is no longer available. 46 alternative formats are available.
Figure B. Alexa Fluor® 700 conjugated Mouse anti Human CD3 clone UCHT1 (MCA463A700) and StarBright Violet 570 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 515 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV515) and StarBright Violet 710 conjugated Mouse anti Human CD27 antibody, clone UCHT1 (MCA755SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 647 conjugated Mouse anti Human CD3 antibody, clone UCHT1(MCA463A647) and StarBright Violet 790 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBV790). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 700 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463A700) and StarBright Violet 475 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 488 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463A488) and StarBright Violet 760 conjugated Mouse anti Human CD27 (MCA755SBV760). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. clone UCHT1 (MCA463A488) and StarBright UltraViolet 665 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBUV665).
All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 610 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV610) and StarBright UltraViolet 445 conjugated Mouse anti Human CD27 (MCA755SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Blue 700 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBB700) and StarBright UltraViolet 510 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBUV510). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. FITC conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463F) and StarBright UltraViolet 575 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBUV575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBUV400) and StarBright UltraViolet 605 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBUV605). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B StarBright UltraViolet 795 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBUV795) and StarBright Blue 580 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. APC conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MC463APC) and StarBright Blue 615 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBB615). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. RPE conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463PE) and StarBright Blue 675 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBB675). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 665 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBUV665) and StarBright Blue 765 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBB765). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBUV400) and StarBright Blue 810 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBB810).All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 488 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463A488) and StarBright Yellow 575 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBY575). All experiments performed on red cell lysed Human blood gated on live single cell lymphocytes, in the presence of 10% Human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Blue 580 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBB580) and StarBright Yellow 720 conjugated Mouse anti Human CD27 antibody, clone LT27(MCA755SBY720). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 475 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV475) and StarBright Yellow 605 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBY605). All experiments performed on human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 605 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBUV605) and StarBright Yellow 665 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBY665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400) and StarBright Yellow 800 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBY800). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 610 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV610) and StarBright Red 775 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBR775). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 670 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV670) and StarBright Red 815 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBR815). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Blue 580 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBB580) and StarBright UltraViolet 740 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBUV740). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 610 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV610) and StarBright Red 715 conjugated Mouse anti Human CD27 antibody, clone LT27 (MCA755SBR715). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400) and StarBright Red 670 conjugated Mouse anti Human CD27 (MCA755SBR670). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Filter by Application:
F Reset| Mouse anti human CD27, clone LT27, recognizes the human T-cell activation antigen, CD27. This antigen is expressed on activated T lymphocytes and belongs to a family of proteins associated with the nerve growth factor receptor. CD27 is expressed by thymocytes and peripheral T cells and also by EBV transformed B cell lines. Mouse anti human CD27, clone LT27 precipitates a ~55 kDa protein under reducing conditions or a ~120 kDa protein under non-reducing conditions. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey Rhesus Monkey Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to StarBright Red 715 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on DEAE from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Immunogen
- Normal human blood lymphocytes.
- Approx. Protein Concentrations
- For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the P3. X 63-Ag8-653 mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Red 715 638 712 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
Neat |
- 1Staining at lower temperatures generally requires longer incubation to reach antibody binding equilibrium, and this effect is more pronounced for StarBright Dyes due to their larger size. For optimum staining, Bio-Rad recommends incubating samples either overnight at 4°C or 1 hour at room temperature.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
References for CD27 antibody
-
Ravoet, A.M. et al. (1989) CD25 mAb: epitopes recognised, effect on lymphocyte activation, mediation of ADCC
in Leucocyte Typing IV: White Cell Differentiation Antigens Eds Knapp, W. et al. Oxford University Press pp 408-411. -
Boxall, S. et al. (2004) Abnormal cell surface antigen expression in individuals with variant CD45 splicing and histiocytosis.
Pediatr Res. 55 (3): 478-84. -
Griebel, P.J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.
Vet Immunol Immunopathol. 119: 115-22. -
Becker, P.D. et al. (2010) Generation of Human Antigen-Specific Monoclonal IgM Antibodies Using Vaccinated ‘‘Human Immune System’’ Mice
PLoS One. 5(10). pii: e13137. -
van Zelm, M.C. et al. (2007) Replication history of B lymphocytes reveals homeostatic proliferation and extensive antigen-induced B cell expansion.
J Exp Med. 204: 645-55. -
Steger, B. et al. (2014) CD4(+)and CD8(+)T-cell reactions against leukemia-associated- or minor-histocompatibility-antigens in AML-patients after allogeneic SCT.
Immunobiology. 219 (4): 247-60.
- UniProt
- P26842
- Entrez Gene
- CD27
- GO Terms
- GO:0005515 protein binding
- GO:0006915 apoptosis
- GO:0005576 extracellular region
- GO:0004888 transmembrane receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006916 anti-apoptosis
- GO:0006917 induction of apoptosis
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0008588 release of cytoplasmic sequestered NF-kappaB
- View More GO Terms
- GO:0016064 immunoglobulin mediated immune response
- GO:0043027 caspase inhibitor activity
- GO:0045579 positive regulation of B cell differentiation
- GO:0046330 positive regulation of JNK cascade
MCA755SBR715
64579594
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