CD45RO antibody | UCHL1

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Mouse anti Human CD45RO:StarBright Violet 670

Product Type: Monoclonal Antibody
Clone: UCHL1
Isotype: IgG2a
Specificity: CD45RO

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MCA461SBV670 Datasheet SDS SDS	F *	100 Tests/0.5ml		Log in
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Anti Human CD45RO Antibody, clone UCHL1 gallery image 1 — Figure A. FITC conjugated mouse anti human CD45RA (MCA88F) and RPE-Alexa647® conjugated Mouse IgG2a isotype control (MCA929A647). Figure B. FITC conjugated mouse anti human CD45RA (MCA88F) and RPE-Cy5 conjugated mouse anti human CD45RO (**MCA461C**). All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A).

Anti Human CD45RO Antibody, clone UCHL1 gallery image 2 — Figure A. A488 conjugated mouse anti human CD45RA (MCA88A488) and RPE conjugated mouse IgG2a isotype control (MCA929PE). Figure B. A488 conjugated mouse anti human CD45RA (MCA88A488) and RPE conjugated mouse anti Human CD45RO (**MCA461PE**). All experiments performed on red cell lysed human blood gated on lymphocytes. Data acquired on the ZE5 cell analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 3 — Figure A. PE conjugated Mouse anti Human CD45RO (**MCA461PE**). Figure B. PE conjugated Mouse anti Human CD45RO (**MCA461PE**) and SBV515 conjugated Mouse anti Human CD45RA (MCA88SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 4 — Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488) and SBV515 conjugated Mouse anti Human CD45RO (**MCA461SBV515**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 5 — Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488) and StarBright Violet 670 conjugated Mouse anti Human CD45RO (**MCA461SBV670**). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 6 — Figure A. StarBright Violet 440 conjugated Mouse anti Human CD45RO (**MCA461SBV440**). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD45RO (**MCA461SBV440**) and StarBright Violet 670 conjugated Mouse anti Human CD45RA (MCA88SBV670). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 7 — Figure A. StarBright Violet 610 conjugated Mouse anti Human CD45RO (**MCA461SBV610**). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD45RO (**MCA461SBV610**) and StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 8 — Figure A. StarBright Blue 700 conjugated Mouse anti Human CD45RA (MCA88SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD45RA (MCA88SBB700) and StarBright Violet 440 conjugated Mouse anti Human CD45RO (**MCA461SBV440**). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 9 — Figure A. StarBright Blue 700 conjugated Mouse anti Human CD45RO (**MCA461SBB700**). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD45RO (**MCA461SBB700**) and StarBright Violet 610 conjugated Mouse anti Human CD45RA (MCA88SBV610). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 10 — Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488) and StarBright Violet 610 conjugated Mouse anti Human CD45RO (**MCA461SBV610**). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 11 — Figure A. StarBright Blue 700 conjugated Mouse anti Human CD45RO (**MCA461SBB700**). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD45RO (**MCA461SBB700**) and StarBright Violet 570 conjugated Mouse anti Human CD45RA (MCA88SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 12 — Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488) and StarBright Violet 570 conjugated Mouse anti Human CD45 (**MCA461SBV570**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 13 — Figure A. StarBright Violet 440 conjugated Mouse anti Human CD45RO (**MCA461SBV440**). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD45RO (**MCA461SBV440**) and StarBright Violet 710 conjugated Mouse anti Human CD45RA (MCA88SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 14 — Figure A. StarBright Violet 515 conjugated Mouse anti Human CD45RA (MCA88SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD45RA (MCA88SBV515) and StarBright Violet 710 conjugated Mouse anti Human CD45RO (**MCA461SBV710**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 15 — Figure A. StarBright Violet 610 conjugated Mouse anti Human CD45RA (MCA88SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD45RA (MCA88SBV610) and StarBright Violet 790 conjugated Mouse anti Human CD45RO (**MCA461SBV790**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 16 — Figure A. PE conjugated Mouse anti Human CD45RO (**MCA461PE**). Figure B. PE conjugated Mouse anti Human CD45RO (**MCA461PE**) and StarBright Violet 790 conjugated Mouse anti Human CD45RA (MCA88SBV790). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 17 — Figure A. StarBright Violet 710 conjugated Mouse anti Human CD45RO (**MCA461SBV710**). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD45RO (**MCA461SBV710**) and StarBright Violet 475 conjugated Mouse anti Human CD45RA (MCA88SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 18 — Figure A. StarBright Blue 700 conjugated Mouse anti Human CD45RA (MCA88SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD45RA (MCA88SBB700) and StarBright Violet 475 conjugated Mouse anti Human CD45RO (**MCA461SBV475**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 19 — Figure A. StarBright Blue 700 conjugated Mouse anti Human CD45RA (MCA88SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD45RA (MCA88SBB700) and StarBright UltraViolet 400 conjugated Mouse anti Human CD45R) (**MCA461SBUV400**). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 20 — Figure A. StarBright Violet 610 conjugated Mouse anti Human CD45RO (**MCA461SBV610**). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD45RO (**MCA461SBV610**) and StarBright UltraViolet 400 conjugated Mouse anti Human CD45RA (MCA88SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 21 — Figure A. StarBright UltraViolet 510 conjugated Mouse anti Human CD45RO (**MCA461SBUV510**). Figure B. StarBright UltraViolet 510 conjugated Mouse anti Human CD45RO (**MCA461SBUV510**) and StarBright Violet 760 conjugated Mouse anti Human CD45RA (MCA88SBV760). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 22 — Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD45RA (MCA88SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD45RA (MCA88SBUV400) and StarBright Violet 760 conjugated Mouse anti Human CD45 (**MCA461SBV760**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 23 — Figure A. StarBright Violet 475 conjugated Mouse anti Human CD45RO (**MCA461SBV475**). Figure B. StarBright Violet 475 conjugated Mouse anti Human CD45RO (**MCA461SBV475**) and StarBright UltraViolet 665 conjugated Mouse anti Human CD45RA (MCA88SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 24 — Figure A. StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440) and StarBright UltraViolet 665 conjugated Mouse anti Human CD45RO (**MCA461SBUV665**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 25 — Figure A. StarBright Violet 610 conjugated Mouse anti Human CD45RA (MCA88SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD45RA (MCA88SBV610) and StarBright UltraViolet 445 conjugated Mouse anti Human CD45RO (**MCA461SBUV445**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 26 — Figure A. StarBright Violet 710 conjugated Mouse anti Human CD45RO (**MCA461SBV710**). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD45RO (**MCA461SBV710**) and StarBright UltraViolet 445 conjugated Mouse anti Human CD45RA (MCA88SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 27 — Figure A. StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440) and StarBright UltraViolet 795 conjugated Mouse anti human CD45RO (**MCA461SBUV795**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 28 — Figure A. StarBright Violet 475 conjugated Mouse anti Human CD45RA (**MCA461SBV475**). Figure B. StarBright Violet 475 conjugated Mouse anti Human CD45RA (**MCA461SBV475**) and StarBright UltraViolet 795 conjugated Mouse anti human CD45RA (MCA88SBUV795). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 29 — Figure A. StarBright Violet 790 conjugated Mouse anti Human CD45RO (**MCA461SBV790**). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD45RO (**MCA461SBV790**) and StarBright UltraViolet 510 conjugated Mouse anti Human CD45RA (MCA88SBUV510). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 30 — Figure A. StarBright UltraViolet 795 conjugated Mouse anti Human CD45RA (MCA88SBUV795). Figure B. StarBright UltraViolet 795 conjugated Mouse anti Human CD45RA (MCA88SBUV795) and StarBright UltraViolet 510 conjugated Mouse anti Human CD45RO (**MCA461SBUV510**). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 31 — Figure A. StarBright Blue 700 conjugated Mouse anti Human CD45RO (**MCA461SBB700**). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD45RO (**MCA461SBB700**) and StarBright UltraViolet 575 conjugated Mouse anti Human CD45RA (MCA88SBUV575). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 32 — Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD45RA (MCA88A700). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD45RA (MCA88A700) and StarBright UltraViolet 575 conjugated Mouse anti Human CD45RO (**MCA461SBUV575**). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 33 — Figure A. StarBright Violet 515 conjugated Mouse anti Human CD45RO (**MCA461SBV515**). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD45RO (**MCA461SBV515**) and StarBright UltraViolet 605 conjugated Mouse anti Human CD45RA (MCA88SBUV605). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 34 — Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD45RA (MCA88A488) and StarBright UltraViolet 605 conjugated Mouse anti Human CD45 (**MCA461SBUV605**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 35 — Figure A. Starbright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440). Figure B. Starbright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440) and StarBright Blue 580 conjugated Mouse anti Human CD45RO (**MCA461SBB580**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 36 — Figure A. StarBright UltraViolet 510 conjugated Mouse anti Human CD45RO (**MCA461SBUV510**). Figure B. StarBright UltraViolet 510 conjugated Mouse anti Human CD45RO (**MCA461SBUV510**) and StarBright Blue 580 conjugated Mouse anti Human CD45RA (MCA88SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 37 — Figure A. StarBright UltraViolet 795 conjugated Mouse anti Human CD45RO (**MCA461SBUV795**). Figure B. StarBright UltraViolet 795 conjugated Mouse anti Human CD45RO (**MCA461SBUV795**) and StarBright Blue 615 conjugated Mouse anti Human CD45RA (MCA88SBB615). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 38 — Figure A. StarBright UltraViolet 795 conjugated Mouse anti Human CD45RA (MCA88SBUV795). Figure B. StarBright UltraViolet 795 conjugated Mouse anti Human CD45RA (MCA88SBUV795) and StarBright Blue 615 conjugated Mouse anti Human CD45RO (**MCA461SBB615**). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 39 — Figure A. RPE conjugated Mouse anti Human CD45RO (**MCA461PE**). Figure B. RPE conjugated Mouse anti Human CD45RO (**MCA461PE**) and StarBright Blue 675 conjugated Mouse anti Human CD45RA (MCA88SBB675). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 40 — Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD45RA (MCA88SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD45RA (MCA88SBUV400) and StarBright Blue 675 conjugated Mouse anti Human CD45RA (**MCA461SBB675**). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 41 — Figure A. StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440) and StarBright Blue 765 conjugated Mouse anti Human CD45RO (**MCA461SBB765**). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 42 — Figure A. StarBright UltraViolet 605 conjugated Mouse anti Human CD45RO (**MCA461SBUV605**). Figure B. StarBright UltraViolet 605 conjugated Mouse anti Human CD45RO (**MCA461SBUV605**) and StarBright Blue 765 conjugated Mouse anti Human CD45RA (MCA88SBB765). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 43 — Figure A. StarBright Violet 515 conjugated Mouse anti Human CD45RA (MCA88SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD45RA (MCA88SBV515) and StarBright Blue 810 conjugated Mouse anti Human CD45RO (**MCA461SBB810**). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 44 — Figure A. StarBright Violet 440 conjugated Mouse anti Human CD45RO (**MCA461SBV440**). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD45RO (**MCA461SBV440**) and StarBright Blue 810 conjugated Mouse anti Human CD45RA (MCA88SBB810). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 45 — Figure A. StarBright Violet 710 conjugated Mouse anti Human CD45RO (**MCA461SBV710**). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD45RO (**MCA461SBV710**) and StarBright Yellow 575 conjugated Mouse anti Human CD45RA (MCA88SBY575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 46 — Figure A. StarBright Violet 760 conjugated Mouse anti Human CD45RA (MCA88SBV760). Figure B. StarBright Violet 760 conjugated Mouse anti Human CD45RA (MCA88SBV760) and StarBright Yellow 575 conjugated Mouse anti Human CD45RO (**MCA461SBY575**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 47 — Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD45RO (**MCA461SBUV400**). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD45RO (**MCA461SBUV400**) and StarBright Yellow 720 conjugated Mouse anti Human CD45RA (MCA88SBY720). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 48 — Figure A. StarBright Violet 475 conjugated Mouse anti Human CD45RA (MCA88SBV475). Figure B. StarBright Violet 475 conjugated Mouse anti Human CD45RA (MCA88SBV475) and StarBright Yellow 720 conjugated Mouse anti Human CD45RO (**MCA461SBY720**). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 49 — Figure A. StarBright Blue 765 conjugated Mouse anti Human CD45RO (**MCA461SBB765**). Figure B. StarBright Blue 765 conjugated Mouse anti Human CD45RO (**MCA461SBB765**) and StarBright Yellow 605 conjugated Mouse anti Human CD45RA (MCA88SBY605). All experiments performed on human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 50 — Figure A. StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD45RA (MCA88SBV440) and StarBright Yellow 605 conjugated Mouse anti Human CD45RO (**MCA461SBY605**). All experiments performed on human PBMCs stimulated with PHA for 3 days gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 51 — Figure A. StarBright Violet 790 conjugated Mouse anti Human CD45A (MCA88SBV570). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD45A (MCA88SBV570) and StarBright Yellow 665 conjugated Mouse anti Human CD45RA (**MCA461SBY665**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 52 — Figure A. StarBright Violet 570 conjugated Mouse anti Human CD45RO (**MCA461SBV570**). Figure B. StarBright Violet 570 conjugated Mouse anti Human CD45RO (**MCA461SBV570**) and StarBright Yellow 665 conjugated Mouse anti Human CD45RA (MCA88SBY665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 53 — Figure A. StarBright Yellow 575 conjugated Mouse anti Human CD45RO (**MCA461SBY575**). Figure B. StarBright Yellow 575 conjugated Mouse anti Human CD45RO (**MCA461SBY575**) and StarBright Yellow 800 conjugated Mouse anti Human CD45RA (MCA88SBY800). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 54 — Figure A. StarBright Blue 675 conjugated Mouse anti Human CD45RA (MCA88SBB675). Figure B. StarBright Blue 675 conjugated Mouse anti Human CD45RA (MCA88SBB675) and StarBright Yellow 800 conjugated Mouse anti Human CD45RO (**MCA461SBY800**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 55 — Figure A. StarBright Violet 515 conjugated Mouse anti Human CD45RO (**MCA461SBV515**). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD45RO (**MCA461SBV515**) and StarBright Red 775 conjugated Mouse anti Human CD45RA (MCA88SBR775). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 56 — Figure A. StarBright Violet 610 conjugated Mouse anti Human CD45RA (MCA88SBV515). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD45RA (MCA88SBV515) and StarBright Red 775 conjugated Mouse anti Human CD45RO (**MCA461SBR775**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 57 — Figure A. StarBright Violet 670 conjugated Mouse anti Human CD45RA (MCA88SBV670). Figure B. StarBright Violet 670 conjugated Mouse anti Human CD45RA (MCA88SBV670) and StarBright Red 815 conjugated Mouse anti Human CD45RO (**MCA461SBR815**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 58 — Figure A. StarBright Violet 670 conjugated Mouse anti Human CD45RO (**MCA461SBV670**). Figure B. StarBright Violet 670 conjugated Mouse anti Human CD45RO (**MCA461SBV670**) and StarBright Red 815 conjugated Mouse anti Human CD45RA (MCA88SBR815). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 59 — Figure A. StarBright Blue 580 conjugated Mouse anti Human CD45RO (**MCA461SBB580**). Figure B. StarBright Blue 580 conjugated Mouse anti Human CD45RO (**MCA461SBB580**) and StarBright UltraViolet 740 conjugated Mouse anti Human CD45RA (MCA88SBUV740). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 60 — Figure A. StarBright Blue 580 conjugated Mouse anti Human CD45RA (MCA88SBB580). Figure B. StarBright Blue 580 conjugated Mouse anti Human CD45RA (MCA88SBB580) and StarBright UltraViolet 740 conjugated Mouse anti Human CD45RO (**MCA461SBUV740**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 61 — Figure A. StarBright Violet 610 conjugated Mouse anti Human CD45RO (**MCA461SBV610**). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD45RO (**MCA461SBV610**) and StarBright Red 715 conjugated Mouse anti Human CD45RA (MCA88SBR715). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 62 — Figure A. StarBright Violet 610 conjugated Mouse anti Human CD45RA (MCA88SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD45RA (MCA88SBV610) and StarBright Red 715 conjugated Mouse anti Human CD45RO (**MCA461SBR715**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 63 — Figure A. StarBright UltraViolet 440 conjugated Mouse anti Human CD45RO (**MCA461SBV440**). Figure B. StarBright UltraViolet 440 conjugated Mouse anti Human CD45RO (**MCA461SBV440**) and StarBright Red 670 conjugated Mouse anti Human CD45RA (MCA88SBR670). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 64 — Figure A. StarBright Blue 615 conjugated Mouse anti Human CD45RA (MCA88SBB615). Figure B. StarBright Blue 615 conjugated Mouse anti Human CD45RA (MCA88SBB615) and StarBright Red 670 conjugated Mouse anti Human CD45RO(**MCA461SBR670**). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 65 — Human whole blood was stained with Anti-Human CD45RO StarBright Blue 810 (**MCA461SBB810**) only (Figure A) or with with Anti-Human CD45RO StarBright Blue 810 (**MCA461SBB810**) and Anti-Human CD45RA StarBright UltraViolet 540 (**MCA88SBUV540**) (Figure B). Experiments were performed on isolated red-cell lysed blood, gated on live, single monocytes. Data were acquired on a Cytek Aurora.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 66 — Human whole blood was stained with Anti-Human CD45RO StarBright Blue 810 (**MCA461SBB810**) only (Figure A) or with with Anti-Human CD45RO StarBright Blue 810 (**MCA461SBB810**) and Anti-Human CD45RA StarBright UltraViolet 700 (**MCA88SBUV700**) (Figure B). Experiments were performed on isolated red-cell lysed blood, gated on live, single monocytes. Data were acquired on a Cytek Aurora.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 67 — Human whole blood was stained with Anti-Human CD45RO StarBright Blue 615 (**MCA461SBB615**) only (Figure A) or with with Anti-Human CD45RO StarBright Blue 615 (**MCA461SBB615**) and Anti-Human CD45RA StarBright UltraViolet 770 (**MCA88SBUV770**) (Figure B). Experiments were performed on isolated red-cell lysed blood, gated on live, single monocytes. Data were acquired on a Cytek Aurora.

Anti Human CD45RO Antibody, clone UCHL1 gallery image 68 — Human whole blood was stained with Anti-Human CD45RO StarBright Blue 810 (**MCA461SBB810**) only (Figure A) or with with Anti-Human CD45RO StarBright Blue 810 (**MCA461SBB810**) and Anti-Human CD45RA StarBright Violet 535 (**MCA88SBV535**) (Figure B). Experiments were performed on isolated red-cell lysed blood, gated on live, single monocytes. Data were acquired on a Cytek Aurora.

Filter by Application:

Mouse anti Human CD45RO monoclonal antibody, clone UCHL1 recognizes the low molecular weight isoform (180 kDa) of the leucocyte common antigen (LCA). The antigen is expressed by a functional subset of T cells with memory phenotype.

In peripheral blood Mouse anti Human CD45RO, clone UCHL1 stains 40-80% of lymphocytes and all monocytes and granulocytes.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Chimpanzee

Marmoset; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG conjugated to StarBright Violet 670 - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen: Human IL-2 dependent T cells
Approx. Protein Concentrations: For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
Fusion Partners: Spleen cells from immunised mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

StarBright Violet 670 401 667
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry ¹		Neat

¹Staining at lower temperatures generally requires longer incubation to reach antibody binding equilibrium, and this effect is more pronounced for StarBright Dyes due to their larger size. For optimum staining, Bio-Rad recommends incubating samples either overnight at 4°C or 1 hour at room temperature.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 5μl of the suggested working dilution to label 0.5x10⁶ cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.

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Source Reference

Smith, S.H. et al. (1986) Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.
Immunology 58: 63-70.

References for CD45RO antibody

Norton, A.J. et al. (1986) Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues.
J Clin Pathol. 39 (4): 399-405.
Terry. L.A. et al. (1987) Phenotypic heterogeneity of the CD4+ and CD8+ subsets.
Leucocyte Typing III. McMichael, A.J., Beverley, P.C.L. et al. eds. University Press. pp 225-7.
Akbar, A.N. et al. (1988) Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells.
J Immunol. 140 (7): 2171-8.
Beverley, P.C.L. et al. (1988) Phenotypic diversity of the CD45 antigen and its relation to function.
Immunology, Suppl. 1: 3-5.
Merkenschlager, M. et al. (1988) Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation.
Eur J Immunol. 18 (11): 1653-61.
Terry, L.A. et al. (1988) The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45.
Immunology. 64 (2): 331-6.
Cavers, M. et al. (2002) Differential expression of beta1 and beta2 integrins and L-selectin on CD4+ and CD8+ T lymphocytes in human blood: comparative analysis between isolated cells, whole blood samples and cryopreserved preparations.
Clin Exp Immunol. 127: 60-5.
Leigh, J.E. et al. (2006) Characterization of the immune status of CD8+ T cells in oral lesions of human immunodeficiency virus-infected persons with oropharyngeal Candidiasis.
Clin Vaccine Immunol. 13: 678-83.

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Arthritis Rheum. 58: 875-87.
Hutnick, N.A. et al. (2010) Vaccination with Ad5 vectors expands Ad5-specific CD8 T cells without altering memory phenotype or functionality.
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