CD195 antibody | HEK/1/85a

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Rat anti Human CD195:StarBright Violet 610

Product Type: Monoclonal Antibody
Clone: HEK/1/85a
Isotype: IgG2a
Specificity: CD195

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References (6)
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Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

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MCA2175SBV610 Datasheet SDS SDS	F *	100 Tests/0.5ml		Log in
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Anti Human CD195 Antibody, clone HEK/1/85a thumbnail image 1

Anti Human CD195 Antibody, clone HEK/1/85a thumbnail image 2

Anti Human CD195 Antibody, clone HEK/1/85a thumbnail image 3

Anti Human CD195 Antibody, clone HEK/1/85a thumbnail image 4

Anti Human CD195 Antibody, clone HEK/1/85a thumbnail image 5

Anti Human CD195 Antibody, clone HEK/1/85a thumbnail image 6

Anti Human CD195 Antibody, clone HEK/1/85a thumbnail image 7

Anti Human CD195 Antibody, clone HEK/1/85a gallery image 1 — **Published customer image:**
Rat anti Human CD195 antibody, clone HEK/1/85a (**MCA2175**) used for the immunoprecipitation of CD195 from transfected CHO lysates.
**Image caption:**
Ligand-elicited recruitment of arrestin2 to CCR5. CHO-CCR5 cells stably transfected with arrestin2-GFP were treated with the indicated chemokines (100 nM, 50 min), washed extensively, crosslinked, washed again and lysed prior to immunoprecipitation with an anti-CCR5 antibody. Immunoprecipitates were subjected to Western blot using antibodies against GFP, arrestin2 and CCR5. CP; total cell pellet control, IP; antibody specificity used for immunoprecipitation, IB; antibody specificity used for immunoblot.

From: Bönsch C, Munteanu M, Rossitto-Borlat I, Fürstenberg A, Hartley O (2015)
Potent Anti-HIV Chemokine Analogs Direct Post-Endocytic Sorting of CCR5.
**PLoS ONE 10(4): e0125396**.
doi: 10.1371/journal.pone.0125396
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Human CD195 Antibody, clone HEK/1/85a gallery image 2 — **Published customer image:**
Rat anti Human CD195 antibody, clone HEK/1/85a (**MCA2175GA**) used for the detection of CD195 expression by transfected CHO cells by immunofluorescence.
**Image caption:**
Spatial and temporal resolution of arrestin2-CCR5 association. A CHO-CCR5 cells stably transfected with arrestin2-GFP were treated with chemokine analogs (100 nM) as indicated and the redistribution of arrestin2-GFP was followed by live fluorescence microscopy. Images captured prior to (0 min) and after ligand treatment (6 min) are shown. B CHO-CCR5 cells stably transfected with arrestin2-GFP (green), preincubated with rhodamine-labeled anti-CCR5 antibody (red), were washed and then incubated 90 min at 37°C with chemokine analogs (100 nM) prior to image capture. Maximal intensity projections are shown. Scale bar = 20 μm.

From: Bönsch C, Munteanu M, Rossitto-Borlat I, Fürstenberg A, Hartley O (2015)
Potent Anti-HIV Chemokine Analogs Direct Post-Endocytic Sorting of CCR5.
**PLoS ONE 10(4): e0125396**.
doi: 10.1371/journal.pone.0125396
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Human CD195 Antibody, clone HEK/1/85a gallery image 3 — Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (**MCA463SBV440**).
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 antibody, clonw UCHT1 (**MCA463SBV440**) and StarBright Violet 790 conjugated Rat anti Human CD195 antibody, clone HEK/1/85a (**MCA2175SBV790**).
All experiments performed on human PBMCs gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the **ZE5 Cell Analyzer**.

Anti Human CD195 Antibody, clone HEK/1/85a gallery image 4 — Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (**MCA463SBV440**).
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 antibody, clonw UCHT1 (**MCA463SBV440**) and StarBright Blue 700 conjugated Mouse anti Human CD195 antibody, clone HEK/1/85a (**MCA2175SBB700**).
All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the **ZE5 Cell Analyzer**.

Anti Human CD195 Antibody, clone HEK/1/85a gallery image 5 — Figure A. StarBright Red 670 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (**MCA463SBR670**). Figure B. StarBright Red 670 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (**MCA463SBR670**) and StarBright Violet 570 conjugated Mouse anti Human CD195 antibody, clone HEK/1/85a (**MCA2175SBV570**).
All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the **ZE5 Cell analyser**.

Anti Human CD195 Antibody, clone HEK/1/85a gallery image 6 — Figure A. StarBright Red 670 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (**MCA463SBR670**).
Figure B. StarBright Red 670 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (**MCA463SBR670**) and StarBright Violet 440 conjugated Rat anti Human CD195 (**MCA2175SBV440**). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the **ZE5 Cell analyser**.

Anti Human CD195 Antibody, clone HEK/1/85a gallery image 7 — Figure A. StarBright Yellow 800 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (**MCA463SBY800**).
Figure B. StarBright Yellow 800 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (**MCA463SBY800**) and StarBright Violet 610 conjugated Rat anti Human CD195 antibody, clone HEK/1/85a (**MCA2175SBV610**). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the **ZE5 Cell analyser**.

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IP IF F

Rat anti Human CD195 antibody, clone HEK/1/85a recognizes the human CD195 cell surface antigen, a 45 kDa glycoprotein also known as CCR5.

CD195 acts as a receptor for a number of chemokines including RANTES and eotaxin and serves as a co-receptor for the entry of HIV into cells. CD195 is expressed by a subset of T lymphocytes and by monocytes.

Target Species: Human
Product Form: Purified IgG conjugated to StarBright Violet 610 - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen: CHO cells transfected with human CCR5
Approx. Protein Concentrations: For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
Fusion Partners: Spleen cells from immunized rats were fused with cell of the rat Y3 myeloma cell line
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

StarBright Violet 610 403 607
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry ¹		Neat

¹Staining at lower temperatures generally requires longer incubation to reach antibody binding equilibrium, and this effect is more pronounced for StarBright Dyes due to their larger size. For optimum staining, Bio-Rad recommends incubating samples either overnight at 4°C or 1 hour at room temperature.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 5μl of the suggested working dilution to label 0.5x10⁶ cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.

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Human Seroblock	BUF070B	F	200 Test	Log in
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References for CD195 antibody

Mueller, A. et al. (2002) Pathways for internalization and recycling of the chemokine receptor CCR5.
Blood 99 (3): 785-91.
Ramirez, R. et al. (2006) Microinflammation in hemodialysis is related to a preactivated subset of monocytes.
Hemodial Int. 10 Suppl 1:S24-7.
Liptrott, N.J. et al. (2009) The impact of cytokines on the expression of drug transporters, cytochrome P450 enzymes and chemokine receptors in human PBMC.
Br J Pharmacol. 156: 497-508.
Pilch-Cooper, H.A. et al. (2011) Circulating human CD4 and CD8 T cells do not have large intracellular pools of CCR5.
Blood 118: 1015-9.
Bönsch, C. et al. (2015) Potent Anti-HIV Chemokine Analogs Direct Post-Endocytic Sorting of CCR5.
PLoS One 10 (4): e0125396.
Holmannova, D. et al. (2017) Effects of conventional CPB and mini-CPB on neutrophils CD162, CD166 and CD195 expression.
Perfusion. 32 (2): 141-50.

Synonyms: CCR5
RRID: AB_2943391
UniProt: P51681
Entrez Gene: CCR5