CD11b antibody | CC126
Mouse anti Bovine CD11b:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- CC126
- Isotype
- IgG2b
- Specificity
- CD11b
Antibody Quality and Performance Guarantee
Figure B. RPE conjugated Mouse anti Bovine CD335 antibody, clone AKS1 (MCA2365PE) and FITC conjugated Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425F). All experiments performed on red cell lysed bovine blood gated on live cells in the presence of 10% bovine serum.
Data acquired on the ZE5 Cell analyser.
Figure B. Purified Mouse anti Bovine CD13 antibody, clone CC81 (MCA2338GA) detected with RPE conjugated Goat anti Mouse IgG1 (STAR132PE) and FITC conjugated Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Data acquired on the ZE5 Cell analyser.
Figure B. FITC conjugated Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425F) and purified Mouse anti Bovine CD13 antibody, clone CC81 (MCA2338GA) detected with RPE conjugated Goat anti Mouse IgG1 (STAR132PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Data acquired on the ZE5 Cell analyser.
Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425) used as a blocking antibody preventing Emeria bovis infection of bovine neutrophils.
Image caption:
CD11b surface expression and CD11b-dependent-NET formation of bovine E. bovis sporozoite-exposed PMN. (A) Bovine PMN were exposed to CFSE-labeled sporozoites (1:1) and subjected to FACS analysis probing with an antibody directed against CD11b. Non-exposed PMN in plain medium served as negative controls. The histograms represent the shift in the mean fluorescence of the PMN population analyzed and are representative of the experiments performed. (B) NET formation in the presence of anti-CD11b antibodies. Bovine PMN were pre-treated with anti-CD11b prior to sporozoite exposure. Stimulation with zymosan served as a positive control; PMN in plain medium were used for negative controls.
From: Muñoz-Caro et al.
Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent.
Veterinary Research 2015 46:23.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425) used for the detection of CD11b on ovine leukocytes by flow cytometry
Image caption:
Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of γδ T-cells and B-cells respectively. animals.
From: Connelley T, Storset AK, Pemberton A, MacHugh N, Brown J, Lund H, Morrison IW.
NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 2011 Feb 23;42:37.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425GA) used for gating bovine adipose tissue derived cells by flow cytometry.
Image caption:
Flow cytometry gating strategy used to define cell populations from the stromal vascular fraction of bovine subcutaneous adipose tissue. (a) Time parameter allowed exclusion of events bursts (1). (b) Selection of cells without debris (2) and (c) singlets (3). (d) Dead cells exclusion with Fixable Viability Dye (FVD) (4). (e) Selection of CD45+ (5) and CD45− (6) cells. (f) Selection of CD14+CD11b+(7) or CD11b−/+CD14− cells (8). (g) Gate used to analyse CD14+CD11b+MHC-II+CD45+ cells (9) (macrophages). (h) Gate used to identify CD11c+ (10) and CD11c− (11) macrophages. (i) Selection of SSC-Ahigh MHC-II− cells (12). (j) Gate used to analyse SSC-AhighCD11b−/+CD14−MHC-II−CH138A−CD45+ cells (13) (mast cells). (k) Selection of CD11b+ (14) or CD11b− (15) cells in CD45− cells. (l) Gate used to analyse CH138A+CD11b+ cells (16) (neutrophils). (m) Gate used to analyse CH138A+CD11b−MHC-II−CD45- cells (17). Pseudocolor plots are representative examples of the analysis of SVF cells isolated from SAT.
From: Oliveira BM, Pinto A, Correia A, Ferreira PG, Vilanova M, Teixeira L.
Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows.
Sci Rep. 2020 Feb 4;10(1):1771.
doi: 10.1038/s41598-020-58678-0.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425GA) used for the evaluation of CD11b in dairy cows by immunofluoresecence and western blotting.
Image caption:
Role of autophagy in histamine (HIS) regulating bovine neutrophil adhesion in vitro; chloroquine (CQ) was used to inhibit autophagy. (F) Immunofluorescence data showing the co-localization of CD11b with microtubule associated proteins 1A/1B light chain 3 (LC3) in neutrophils [magnification 4,000×; nuclei stained with 4′,6-diamidino-2-phenylindole (DAPI) are in blue, CD11b is in green, and LC3 is in red]. Images represent the typical common phenotype of each group.
Enhanced adhesion ability of neutrophils of cows with SARA. (C) protein abundance of membrane CD11b (integrin alpha-M precursor) and CD11a (integrin alpha-L precursor), showing representative blots in both groups (n = 10/group; 3 wells of each group represent 3 different samples).
From: Wang K, Sun Z, Li Y, Liu M, Loor JJ, Jiang Q, Liu G, Wang Z, Song Y, Li X.
Histamine promotes adhesion of neutrophils by inhibition of autophagy in dairy cows with subacute ruminal acidosis.
J Dairy Sci. 2022 Sep;105(9):7600-7614.
doi: 10.3168/jds.2022-22036.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425GA) used to label sheep peripheral blood mononuclear cells by flow cytometry.
Image caption:
Gating Strategy forFlow Cytometry:
More that 10,000 events were acquired ba a Macsquant flow cytometer for each sample and analysed with Flowlogic software using the following strategy.
The main cell population was selected using side scatter - Area (SS-A) versus FSC-A in P1 gate. Doublet cells were excluded in gate P2. Cells from P1/P2 gate were analsed for the specific staining as shown in histogram overlays showing staining with specific antibodies (in red) in comparison with their respective isotypes (in blue). Markers (M, M1, M2, M3, M4, M5) were set up according to the control isotypes to determine the percentage of positive cells. The same strategy was applied to all samples.
From: Ducournau C, Moiré N, Carpentier R, Cantin P, Herkt C, Lantier I, Betbeder D, Dimier-Poisson I.
Effective Nanoparticle-Based Nasal Vaccine Against Latent and Congenital Toxoplasmosis in Sheep.
Front Immunol. 2020 Sep 9;11:2183.
doi: 10.3389/fimmu.2020.02183.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
FITC conjugated Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425F) used for the analysis of CD11b expression on ovine and bovine cells by flow cytometry.
Image caption:
Identification of neonatal intestinal mononuclear phagocytes in the ileal Peyer’s patch of lambs and calves. Intestinal tissues from the ileal Peyer’s patch (IPP) were recovered from 10-day-old lambs (n = 6) and calves (n = 10). Mechanical and enzymatic dissociations were performed to obtain total isolated intestinal cells, then stained for flow cytometry. Representative gating for mononuclear phagocytes (MP) subsets are shown for the IPP of lamb (A) and calf (B). Following exclusion of dead cells, CD11c+MHCII+ cells were gated to select total MP and then four subsets were distinguished based on surface expression of CD14, CD172α, CD11b, and Cadm1: CD14+CD172α+Cadm1intCD11b+ (subset 1, green), CD14−CD172α−Cadm1+CD11b− (subset 2, purple), CD14−CD172α+Cadm1intCD11b+ (subset 3, blue), and CD14−CD172α+/-Cadm1−CD11b− (subset 4, yellow). Subsets 1, 2, and 3 of MP from the IPP of a 10-day-old lamb were cell sorted by flow cytometry, cytospined, and stained with hematoxylin and eosin. Pictures of the three subsets of MP were obtained by optical microscopy (A, right panel). Pie charts represent the cell proportions in percentage of each intestinal MP subset among viable CD11c+MHCII+ cells (mean ± SD) for lambs (n = 6; A, bottom panel) and calves (n = 10; B, bottom panel).
From: Baillou A, Tomal F, Chaumeil T, Barc C, Levern Y, Sausset A, Pezier T, Schulthess J, Peltier-Pain P, Laurent F and Lacroix-Lamandé S
Characterization of intestinal mononuclear phagocyte subsets in young ruminants at homeostasis and during Cryptosporidium parvum infection.
Front. Immunol. (2024) 15:1379798.
doi: 10.3389/fimmu.2024.1379798
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Figure B. Alexa Flour® 647 conjugated Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425A647) and StarBright Violet 570 conjugated Mouse anti Bovine CD14 antibody, clone CC-G33 (MCA2678SBV570).
All experiments performed on red cell lysed bovine blood gated on live cells in the presence of 10% bovine serum. Data acquired on the ZE5 Cell analyser.
Figure B. Alexa Flour® 647 conjugated Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425A647) and StarBright Violet 440 conjugated Mouse anti Bovine CD14 antibody, clone CC-G33 (MCA2678SBV440). All experiments performed on red cell lysed bovine blood gated on live cells in the presence of 10% bovine serum.
Data acquired on the ZE5 Cell analyser.
Figure B. Alexa Flour® 647 conjugated Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425A647) and StarBright Violet 610 conjugated Mouse anti Bovine CD14 antibody, clone CC-G33 (MCA2678SBV610). All experiments performed on red cell lysed bovine blood gated on live cells in the presence of 10% bovine serum.
Data acquired on the ZE5 Cell analyser.
Filter by Application:
F BA IF WB Reset| Mouse anti Bovine CD11b antibody, clone CC126 recognizes the bovine CD11b cell surface antigen. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2b Negative Control:Alexa Fluor® 647 | MCA691A647 | F | 100 Tests/1ml |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2b Negative Control:Alexa Fluor® 647 | ||||||
References for CD11b antibody
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Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47. -
Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
Vet Immunol Immunopathol. 39 (1-3): 283-90. -
McClenahan, D.J. et al. (2000) Association among filamentous actin content, CD11b expression, and membrane deformability in stimulated and unstimulated bovine neutrophils.
Am J Vet Res. 61 (4): 380-6. -
Summers, C. et al. (2005) An influx of macrophages is the predominant local immune response in ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 106 (3-4): 285-94. -
Dedieu, L. et al. (2005) Mycoplasma mycoides ssp. mycoides biotype small colony-secreted components induce apoptotic cell death in bovine leucocytes.
Scand J Immunol. 62 (6): 528-38. -
Fach, S.J. et al. (2006) Pulmonary dendritic cells isolated from neonatal and adult ovine lung tissue.
Vet Immunol Immunopathol. 112 (3-4): 171-82. -
Lamote, I. et al. (2006) Influence of sex steroids on the viability and CD11b, CD18 and CD47 expression of blood neutrophils from dairy cows in the last month of gestation.
Vet Res. 37 (1): 61-74. -
Fach, S.J. et al. (2007) Neonatal ovine pulmonary dendritic cells support bovine respiratory syncytial virus replication with enhanced interleukin (IL)-4 And IL-10 gene transcripts.
Viral Immunol. 20 (1): 119-30.
View The Latest Product References
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Graff, J.C. and Jutila, M.A. (2007) Differential regulation of CD11b on gammadelta T cells and monocytes in response to unripe apple polyphenols.
J Leukoc Biol. 82: 603-7. -
Foulon, E. & Foucras, G. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
J Immunol Methods. 339 (1): 1-10. -
Reber, A.J. et al. (2008) Transfer of maternal colostral leukocytes promotes development of the neonatal immune system I. Effects on monocyte lineage cells.
Vet Immunol Immunopathol. 123 (3-4): 186-96. -
Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
Dev Comp Immunol. 32: 837-49. -
Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytes and modulate CD18 expression.
Vet Res. 39: 50. -
Sassa, Y. et al. (2010) Bovine macrophage degradation of scrapie and BSE PrPSc.
Vet Immunol Immunopathol. 133 (1): 33-9. -
Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.
Virology. 405 (1): 110-9. -
Top, S. et al. (2011) Infection of nonhost species dendritic cells in vitro with an attenuated myxoma virus induces gene expression that predicts its efficacy as a vaccine vector.
J Virol. 85: 12982-94. -
Albenzio, M. & Caroprese, M. (2011) Differential leucocyte count for ewe milk with low and high somatic cell count.
J Dairy Res. 78 (1): 43-8. -
Kallapur, S.G .et al. (2011) Pulmonary and systemic inflammatory responses to intra-amniotic IL-1α in fetal sheep.
Am J Physiol Lung Cell Mol Physiol. 301 (3): L285-95. -
Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 42: 37. -
Albenzio, M. et al. (2012) Immune competence of the mammary gland as affected by somatic cell and pathogenic bacteria in ewes with subclinical mastitis.
J Dairy Sci. 95: 3877-87. -
Ozawa, T. et al. (2012) Effect of intramammary infusion of rbGM-CSF on SCC and expression of polymorphonuclear neutrophil adhesion molecules in subclinical mastitis cows.
Vet Res Commun. 36 (1): 21-7. -
Pilla, R. et al. (2013) Differential cell count as an alternative method to diagnose dairy cow mastitis.
J Dairy Sci. 96 (3): 1653-60. -
Zoldan, K. et al. (2014) Increase of CD25 expression on bovine neutrophils correlates with disease severity in post-partum and early lactating dairy cows.
Dev Comp Immunol. 47 (2): 254-63. -
Godoy, R.F. et al. (2014) Do progenitor cells from different tissue have the same phenotype?
Res Vet Sci. 96 (3): 454-9. -
Flameng, W. et al. (2014) Coating with fibronectin and stromal cell-derived factor-1α of decellularized homografts used for right ventricular outflow tract reconstruction eliminates immune response-related degeneration.
J Thorac Cardiovasc Surg. 147 (4): 1398-1404.e2. -
Muñoz-Caro, T. et al. (2015) Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent.
Vet Res. 46: 23. -
Conejeros, I. et al. (2015) Effect of the synthetic Toll-like receptor ligands LPS, Pam3CSK4, HKLM and FSL-1 in the function of bovine polymorphonuclear neutrophils.
Dev Comp Immunol. 52 (2): 215-25. -
Weber, B. et al. (2016) In vitro fabrication of autologous living tissue-engineered vascular grafts based on prenatally harvested ovine amniotic fluid-derived stem cells.
J Tissue Eng Regen Med. 10 (1): 52-70. -
Merriman, K.E. et al. (2018) Intramammary 25-hydroxyvitamin D3 treatment modulates innate immune responses to endotoxin-induced mastitis.
J Dairy Sci. 101 (8): 7593-607. -
Brodzki, P. et al. (2019) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
Anim Sci J. Nov 06 [Epub ahead of print]. -
Cangiano, L.R. et al. (2019) A bioactive extract from Olea europaea. protects newly weaned beef heifers against experimentally induced chronic inflammation.
J Anim Sci. 97 (10): 4349-4361. -
Poindexter, M.B. et al. (2020) Feeding supplemental 25-hydroxyvitamin D3 increases serum mineral concentrations and alters mammary immunity of lactating dairy cows.
J Dairy Sci. 103 (1): 805-22. -
Ducournau, C. et al. (2020) Effective Nanoparticle-Based Nasal Vaccine Against Latent and Congenital Toxoplasmosis in Sheep.
Front Immunol. 11: 2183. -
Risalde, M.A. et al. (2020) BVDV permissiveness and lack of expression of co-stimulatory molecules on PBMCs from calves pre-infected with BVDV.
Comp Immunol Microbiol Infect Dis. 68: 101388. -
Oliveira, B.M. et al. (2020) Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows.
Sci Rep. 10 (1): 1771. -
Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
Ticks Tick Borne Dis. 11 (3): 101365. -
Wang, K. et al. (2022) Histamine promotes adhesion of neutrophils by inhibition of autophagy in dairy cows with subacute ruminal acidosis.
J Dairy Sci. 105 (9): 7600-14. -
Stabel, J.R. et al. (2022) B cell phenotypes and maturation states in cows naturally infected with Mycobacterium avium subsp. Paratuberculosis.
PLoS One. 17 (12): e0278313. -
Baillou, A. et al. (2024) Characterization of intestinal mononuclear phagocyte subsets in young ruminants at homeostasis and during Cryptosporidium parvum infection
Front Immunol 15: 1379798 [Epub ahead of print]. -
Brodzki, P. et al. (2024) The influence of probiotic administration on selected leukocyte subpopulations and the serum amyloid A concentration in the peripheral blood of dairy cows during different lactation periods
Journal of Veterinary Research. 09 Oct [Epub ahead of print]. -
Etchevers, L. et al. (2024) Altered expression of systemic and ovarian factors involved in the recruitment and activation of immune cells in bovine cystic ovarian disease.
Theriogenology. 234: 92-100.
- Synonyms
- Integrin Alpha M Chain
- MAC-1
MCA1425A647
152536
172003
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