CD8 antibody | LT8
Mouse anti Human CD8:StarBright Yellow 665
- Product Type
- Monoclonal Antibody
- Clone
- LT8
- Isotype
- IgG1
- Specificity
- CD8
Antibody Quality and Performance Guarantee
Mouse anti Human CD8 antibody, clone LT8 (MCA1226GA) used for the evaluation of CD8α exptession on T lymphocytes from marmoset blood, spleen and lung by flow cytometry.
Image caption:
The percentage of the total leucocyte count for various cell types identified in naive marmosets (a) cells in naïve blood, (b) in naive spleens, and (c) in naive lungs. CD8 T cells are expressed as a percentage of CD3+ cells. Bar represents the median value.
From: M. Nelson and M. Loveday,
“Exploring the Innate Immunological Response of an Alternative Nonhuman Primate Model of Infectious Disease; the Common Marmoset,”
Journal of Immunology Research, vol. 2014, Article ID 913632, 8 pages, 2014.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Figure B. FITC conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463F) and APC conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226APC). All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A).
Alexa Fluor®700 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226A700) used for the evaluation of CD8 expression on PBMC by flow cytometry.
Image caption:
Gating stategy for cell surface marker analysis in uncultured and cultured PBMC. Gates were applied in the numerical order shown.
From: Manivannan K, Rowan AG, Tanaka Y, Taylor GP, Bangham CRM (2016)
CADM1/TSLC1 Identifies HTLV-1-Infected Cells and Determines Their Susceptibility to CTL-Mediated Lysis.
PLoS Pathog 12(4): e1005560.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Figure B. Alexa Fluor® 488 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463A488) and Alexa Fluor® 647 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226A647). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 647 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463A647) and StarBright Violet 515 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 488 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463A488) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright Violet 515 conjugated streptavidin (STAR210SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 488 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463A488) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright Violet 440 conjugated streptavidin (STAR210SBV440). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 488 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463A488) and StarBright Violet 570 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 488 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463A488) and StarBright Violet 570 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 488 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463A488) and StarBright Violet 710 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 488 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463A488) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright Violet 710 conjugated streptavidin (STAR210SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV440) and StarBright Violet 790 conjugated Mouse anti Human CD8 antiody, clone LT8 (MCA1226SBV790). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 488 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463A488) and StarBright Violet 475 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 790 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV790) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright UltraViolet 400 conjugated streptavidin (STAR210SBUV400). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. RPE-Cy5 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463C) and StarBright UltraViolet 400 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBUV400). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBUV400) and StarBright Violet 760 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBV760). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBUV400) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright Violet 760 conjugated streptavidin (STAR210SBV760). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 475 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV475) and StarBright UltraViolet 665 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 515 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV515) and StarBright UltraViolet 795 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBUV795). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 647 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463A647) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright UltraViolet 795 conjugated streptavidin (STAR210SBUV795). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Blue 700 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBB700) and StarBright UltraViolet 510 conjugated Mouse anti human CD8 antibody, clone LT8 (MCA1226SBUV510). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 710 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV710) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright UltraViolet 510 antibody, clone LT8 (STAR210SBUV510). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 710 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV710) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright UltraViolet 575 conjugated streptavidin (STAR210SBUV575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBUV400) and StarBright UltraViolet 575 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBUV575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV440) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright UltraViolet 605 conjugated streptavidin (STAR210SBUV605). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Blue 700 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBB700) and StarBright UltraViolet 605 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBUV605). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 795 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBUV795) and StarBright Blue 580 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV440) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright Blue 580 conjugated streptavidin (STAR210SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBUV400) and StarBright Blue 615 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBB615). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBUV400) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright Blue 615 conjugated streptavidin (STAR210SBB615). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBUV400) and StarBright Blue 675 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBB675). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. RPE conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463PE) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright Blue 675 conjugated streptavidin (STAR210SBB675). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 665 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBUV665) and StarBright Blue 765 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBB765). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. APC conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463APC) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright Blue 765 conjugated streptavidin (STAR210SBB765). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 760 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV760) and StarBright Yellow 575 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBY575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 795 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBUV795) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright Yellow 575 conjugated streptavidin (STAR210SBY575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 610 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV610) and StarBright Yellow 720 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBY720). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Blue 765 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBB765) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright Yellow 720 conjugated streptavidin (STAR210SBY720).All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Blue 810 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBB810) and StarBright Yellow 605 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBY605). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBUV400) and StarBright Yellow 665 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBY665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBUV400) and StarBright Yellow 800 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBY800). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 610 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV610) and StarBright Red 775 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBR775). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 610 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV440) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright Red 775 conjugated streptavidin (STAR210SBR775). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 440 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV440) and StarBright Red 815 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBR815). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV440) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright Violet 815 conjugated streptavidin (STAR210SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Blue 580 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBB580) and StarBright UltraViolet 740 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBUV740). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 610 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV610) and StarBright Red 715 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBR715). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 610 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV610) and biotin conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226B) detected with StarBright Red 775 conjugated streptavidin (STAR210SBR715). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Yellow 605 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBY605) and StarBright Red 670 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBR670). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 647 conjugated Rat anti Human CD3 antibody, clone CD3-12 (MCA1477A647) and RPE conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226PE). All experiments performed on red cell lysedand permeabilized Human peripheral blood gated on leukocytes in the presence of Human SeroBlock (BUF070A).
Figure B. StarBright Blue 700 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBB700) and StarBright Violet 440 conjugated Mouse anti Human CD8 antibody, clone LT8 (MCA1226SBV440). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 488 conjugated Mouse anti human CD3 (MCA463A488) and StarBright Violet 610 conjugated Mouse anti Human CD8 (MCA1226SBV610). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 790 conjugated Mouse anti Human CD3 antibody, clone UCHT1 (MCA463SBV790) and StarBright UltraViolet 445 conjugated Mouse anti Human CD8 (MCA1226SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Filter by Application:
C P F Reset| Mouse anti Human CD8 antibody, clone LT8 recognizes the human CD8 cell surface glycoprotein expressed by a subset of peripheral blood T cells which express cytotoxic/suppressor activity. It is also expressed weakly on NK cells. The CD8 antigen is a co-receptor for MHC Class I in conjunction with the T cell receptor, and is important in the selection process of CD8+ MHC Class I restricted T cells. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Marmoset Chimpanzee Cynomolgus monkey Red-bellied Tamarin - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to StarBright Yellow 665 - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Immunogen
- Normal human peripheral blood lymphocytes.
- Approx. Protein Concentrations
- For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Yellow 665 554 670 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
Neat |
- 1Staining at lower temperatures generally requires longer incubation to reach antibody binding equilibrium, and this effect is more pronounced for StarBright Dyes due to their larger size. For optimum staining, Bio-Rad recommends incubating samples either overnight at 4°C or 1 hour at room temperature.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
References for CD8 antibody
-
Zarkesh-Esfahani, H. et al. (2001) High-dose leptin activates human leukocytes via receptor expression on monocytes.
J Immunol. 167 (8): 4593-9. -
Manninen, A. & Saksela, K. (2002) HIV-1 Nef interacts with inositol trisphosphate receptor to activate calcium signaling in T cells.
J Exp Med. 195 (8): 1023-32. -
Parnes, J.R. (1989) Molecular biology and function of CD4 and CD8.
Adv Immunol. 44: 265-311. -
Kap, Y.S. et al. (2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
J Histochem Cytochem. 57: 1159-67. -
Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
BMC Immunol. 12: 2. -
Nelson, M. et al. (2010) Characterization of lethal inhalational infection with Francisella tularensis in the common marmoset (Callithrix jacchus).
J Med Microbiol. 59: 1107-13. -
Gibbings, D.J. et al. (2007) CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses.
BMC Immunol. 8: 12. -
Junker, A. et al. (2007) Multiple sclerosis: T-cell receptor expression in distinct brain regions.
Brain. 130: 2789-99.
View The Latest Product References
-
Held, K. et al. (2011) Expression of herpes simplex virus 1-encoded microRNAs in human trigeminal ganglia and their relation to local T-cell infiltrates.
J Virol. 85 (19): 9680-5. -
Hood SP et al. (2014) Changes in immune cell populations in the periphery and liver of GBV-B-infected and convalescent tamarins (Saguinus labiatus).
Virus Res. 179: 93-101. -
Nelson, M. & Loveday, M. (2014) Exploring the innate immunological response of an alternative nonhuman primate model of infectious disease; the common marmoset.
J Immunol Res. 2014: 913632. -
Manivannan, K. et al. (2016) CADM1/TSLC1 Identifies HTLV-1-Infected Cells and Determines Their Susceptibility to CTL-Mediated Lysis.
PLoS Pathog. 12 (4): e1005560. -
Gross, C.C. et al. (2016) Impaired NK-mediated regulation of T-cell activity in multiple sclerosis is reconstituted by IL-2 receptor modulation.
Proc Natl Acad Sci U S A. 113 (21): E2973-82. -
Dunham, J. et al. (2016) Blockade of CD127 Exerts a Dichotomous Clinical Effect in Marmoset Experimental Autoimmune Encephalomyelitis.
J Neuroimmune Pharmacol. 11 (1): 73-83. -
Bughani, U. et al. (2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
PLoS One. 12 (7): e0180088. -
Philippens, I.H. et al. (2017) Acceleration of Amyloidosis by Inflammation in the Amyloid-Beta Marmoset Monkey Model of Alzheimer's Disease.
J Alzheimers Dis. 55 (1): 101-113. -
Flak, V.R. et al. (2024) Pembrolizumab followed by irreversible electroporation of a liver metastasis in pancreatic cancer patients
iScience. 27 (10): 111026.
- UniProt
- P01732
- P10966
- Entrez Gene
- CD8A
- CD8B
- GO Terms
- GO:0005576 extracellular region
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0005887 integral to plasma membrane
- GO:0015026 coreceptor activity
- GO:0019882 antigen processing and presentation
- GO:0042101 T cell receptor complex
- GO:0042288 MHC class I protein binding
- GO:0050776 regulation of immune response
MCA1226SBY665
100007716
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