Cathepsin B
Magic Red™ Cathepsin B Kit
- Product Type
- Kits
- Specificity
- Cathepsin B
The Magic Red Cathepsin B kit uses a quick and easy method to analyze intracellular Cathepsin B protease activity in whole living cells. |
- Reagents In The Kit
-
ICT938: Magic Red Substrate MR-(RR)2, 100 Tests - lyophilized
Hoechst 33342, 1 mL
Acridine Orange, 0.5 ml. -
ICT937: Magic Red Substrate (MR-RR2), 25 Tests - lyophilized
Hoechst 33342, 1 mL
Acridine Orange, 0.5 mL. - Test Principle
- Cathepsin Magic Red kits measure cathepsin B protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product.
The Magic Red reagent contains a cathepsin B target sequence peptide (RR)2 linked to a red (Cresyl Violet) fluorescent probe.
The Cathepsin Magic Red kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.
This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates. - Regulatory
- For research purposes only.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Acknowledgements
- Magic Red is a trademark of Immunochemistry Technologies, LLC.
Once reconstituted with DMSO, use Magic Red reagent immediately, or store at -20°C for 6 months protected from light and thawed no more than twice during that time.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunofluorescence |
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT938.pdf.
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT937.pdf
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Magic Red™ Cathepsin B Kit | ICT938 | IF | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Magic Red™ Cathepsin B Kit |
References for Cathepsin B
-
Hämälistö, S. et al. (2020) Spatially and temporally defined lysosomal leakage facilitates mitotic chromosome segregation.
Nat Commun. 11 (1): 229.
ICT938
152748 153058 156581 158201 158477 158772 158926 161105 162102 163834 164121 164637 166086 167213ICT937
154064 154943 155972 156465 157560 158200 159771 160330 161668 163045 163053 164885 165792 166979If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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