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Tyrosine Hydroxylase (pSer40) antibody

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Anti Tyrosine Hydroxylase (pSer40) Antibody thumbnail image 1
Anti Tyrosine Hydroxylase (pSer40) Antibody gallery image 1
Western blot of phospho- and dephospho-tyrosine hydroxylase demonstrating the selective immunolabeling by the phospho-specific TH Ser40 antibody. Blot probed with Rabbit anti Rat tyrosine hydroxylase (pSer40) antibody (AHP912).

Rabbit anti Tyrosine Hydroxylase (pSer40)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Tyrosine Hydroxylase (pSer40)

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Product Code Applications Pack Size List Price Your Price Qty
AHP912
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C IF WB 0.1 ml loader
List Price Your Price
loader

Rabbit anti Rat tyrosine hydroxylase (pSer40) antibody recognizes tyrosine hydroxylase (TH), also known as tyrosine 3-monooxygenase, when phosphorylated at serine 40.

Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines dopamine (DA), norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain.

Phosphorylation of TH has been shown to occur at several serine residues. Phosphorylation at serine 40 results in an increase in hydroxylase activity, and phosphorylation at serine 19 is reported to promote phosphorylation of the serine 40 residue (Dunkley et al. 2004).

Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson’s disease (Pearson et al 1979).

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Human Expected from Sequence
Pig Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to phosphorylated tyrosine hydroxylase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5
Preservative Stabilisers
0.09%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence within Tyrosine Hydroxylase which includes the phosphorylated residue Ser 40.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/1000
Immunohistology - Frozen 1/1000
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP912 detects a band of approximately 60kDa in PC-12 cell lysates, following stimulation by Okadaic acid.

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References for Tyrosine Hydroxylase (pSer40) antibody

  1. Hoard, J.L. et al. (2008) Cholinergic neurons of mouse intrinsic cardiac ganglia contain noradrenergic enzymes, norepinephrine transporters, and the neurotrophin receptors tropomyosin-related kinase A and p75.
    Neuroscience. 156 (1): 129-42.
  2. Xiao, M.F. et al. (2009) Neural cell adhesion molecule modulates dopaminergic signaling and behavior by regulating dopamine D2 receptor internalization.
    J Neurosci. 29 (47): 14752-63.
  3. Li, S. et al. (2013) The neural cell adhesion molecule (NCAM) associates with and signals through p21-activated kinase 1 (Pak1).
    J Neurosci. 33 (2): 790-803.
  4. Kotarska, A. et al. (2020) Cell adhesion molecule close homolog of L1 binds to the dopamine receptor D2 and inhibits the internalization of its short isoform.
    FASEB J. 34 (4): 4832-51.

Further Reading

  1. Haycock, J.W. (1990) Phosphorylation of tyrosine hydroxylase in situ at serine 8, 19, 31, and 40.
    J Biol Chem. 265 (20): 11682-91.
  2. Bevilaqua, L.R. et al. (2001) Phosphorylation of Ser(19) alters the conformation of tyrosine hydroxylase to increase the rate of phosphorylation of Ser(40).
    J Biol Chem. 276 (44): 40411-6.

Immunofluorescence

RRID
AB_567401
UniProt
P07101
Entrez Gene
TH
GO Terms
GO:0005515 protein binding
GO:0042136 neurotransmitter biosynthetic process
GO:0001666 response to hypoxia
GO:0001963 synaptic transmission, dopaminergic
GO:0003007 heart morphogenesis
GO:0004511 tyrosine 3-monooxygenase activity
GO:0005829 cytosol
GO:0005634 nucleus
GO:0005790 smooth endoplasmic reticulum
GO:0055114 oxidation-reduction process
GO:0006585 dopamine biosynthetic process from tyrosine
GO:0007269 neurotransmitter secretion
GO:0007601 visual perception
GO:0007612 learning
GO:0007613 memory
GO:0007626 locomotory behavior
GO:0008016 regulation of heart contraction
GO:0009898 internal side of plasma membrane
GO:0033162 melanosome membrane
GO:0042418 epinephrine biosynthetic process
GO:0042421 norepinephrine biosynthetic process
GO:0042446 hormone biosynthetic process
GO:0042462 eye photoreceptor cell development
GO:0043204 perikaryon
GO:0043473 pigmentation
GO:0045471 response to ethanol
GO:0048596 embryonic camera-type eye morphogenesis

AHP912

149237 154077 158649

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