Rabbit anti yeast Rad9 antibody detects Saccharomyces cerevisiae Rad9, a protein involved in the DNA damage checkpoint. In S. cerevisiae this checkpoint is mainly controlled by the PI(3) kinase-like kinase (PIKK) Mec1, which is activated in response to DNA damage. Activated Mec1 hyperphosphorylates Rad9, which in turn triggers the binding of Rad53, the orthologue of mammalian Chk2. Activated Rad53 specifically targets substrates required for cell cycle arrest, gene expression and efficient DNA repair. Mutations affecting Rad9 impair checkpoint induced cell-cycle arrest and increase genomic instability.
Rabbit anti yeast Rad9 antibody (AHP1294) is reactive with both the phosphorylated and non-phosphorylated forms of this protein.
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to yeast Rad9 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Synthetic peptide corresponding to aa 1125-1139 of yeast Rad9 protein conjugated to Keyhole Limpet Haemocyanin (KLH).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- GO Terms
double-stranded DNA binding
negative regulation of DNA replication
mitotic cell cycle G1/S transition DNA damage checkpoint
intra-S DNA damage checkpoint
positive regulation of transcription from RNA polymerase II promoter
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of RAD9 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1294 detects a band of approximately 60kDa.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Toh, G.W. & Lowndes, N.F. (2003) Role of the Saccharomyces cerevisiae Rad9 protein in sensing and responding to DNA damage.
Biochem Soc Trans. 31 (Pt 1): 242-6.
Sweeney, F.D. et al. (2005) Saccharomyces cerevisiae Rad9 acts as a Mec1 adaptor to allow Rad53 activation.
Curr Biol. 15 (15): 1364-75.