RAD9 antibody

Rabbit anti Yeast RAD9

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
RAD9

Product Code Applications Pack Size List Price Your Price Qty
AHP1294
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SDS Safety Datasheet SDS
E WB 50 µg loader
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Rabbit anti yeast Rad9 antibody detects Saccharomyces cerevisiae Rad9, a protein involved in the DNA damage checkpoint. In S. cerevisiae this checkpoint is mainly controlled by the PI(3) kinase-like kinase (PIKK) Mec1, which is activated in response to DNA damage. Activated Mec1 hyperphosphorylates Rad9, which in turn triggers the binding of Rad53, the orthologue of mammalian Chk2. Activated Rad53 specifically targets substrates required for cell cycle arrest, gene expression and efficient DNA repair. Mutations affecting Rad9 impair checkpoint induced cell-cycle arrest and increase genomic instability.

Rabbit anti yeast Rad9 antibody (AHP1294) is reactive with both the phosphorylated and non-phosphorylated forms of this protein.

Target Species
Yeast
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to yeast Rad9 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to aa 1125-1139 of yeast Rad9 protein conjugated to Keyhole Limpet Haemocyanin (KLH).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/4000
Western Blotting 1/50 1/250
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1294 detects a band of approximately 60kDa.

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Further Reading

  1. Toh, G.W. & Lowndes, N.F. (2003) Role of the Saccharomyces cerevisiae Rad9 protein in sensing and responding to DNA damage.
    Biochem Soc Trans. 31 (Pt 1): 242-6.
  2. Sweeney, F.D. et al. (2005) Saccharomyces cerevisiae Rad9 acts as a Mec1 adaptor to allow Rad53 activation.
    Curr Biol. 15 (15): 1364-75.

RRID
AB_906114
UniProt
P14737
GO Terms
GO:0000785 chromatin
GO:0003690 double-stranded DNA binding
GO:0042393 histone binding
GO:0005634 nucleus
GO:0006289 nucleotide-excision repair
GO:0007049 cell cycle
GO:0008156 negative regulation of DNA replication
GO:0031571 mitotic cell cycle G1/S transition DNA damage checkpoint
GO:0031573 intra-S DNA damage checkpoint
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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