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WNV antibody

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Anti WNV Core Protein (C-Terminal) Antibody thumbnail image 1 Anti WNV Core Protein (C-Terminal) Antibody thumbnail image 2
Anti WNV Core Protein (C-Terminal) Antibody gallery image 1
Western blot analysis of WNV transfected (B) and untransfected (A) Hela cell lysates probed with Rabbit anti WNV core protein (AHP1666).
Anti WNV Core Protein (C-Terminal) Antibody gallery image 2
Immunofluorescent staining of WNV transfected Vero cells with Rabbit anti WNV core protein (AHP1666).

Rabbit anti WNV Core Protein (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1666
Datasheet
E IF WB
SDS
0.1 mg loader

Rabbit anti West Nile Virus antibody recognizes the ~14 kDa core protein of West Nile Virus (WNV). WNV belongs to the Flaviviridae family which includes closely viruses such as Dengue fever, Yellow fever and Tick borne encephalitis.

West Nile Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope containing envelope and matrix proteins. Research indicates that the viral core protein contributes to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway.

West Nile Virus is spread by mosquitos and is believed to have originated in the West Nile region of Africa but has now spread to Europe, Asia and America where it has become a significant disease affecting human and domestic animal health.

Product Details

Target Species
Viral
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human West Nile Virus core protein was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to an 15 amino acid sequence from near the carboxy terminus of West Nile Virus core protein.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P06935
Entrez Gene
WNVgp1
GO Terms
GO:0005515 protein binding
GO:0003724 RNA helicase activity
GO:0003725 double-stranded RNA binding
GO:0004252 serine-type endopeptidase activity
GO:0003968 RNA-directed RNA polymerase activity
GO:0016021 integral to membrane
GO:0004482 mRNA (guanine-N7-)-methyltransferase activity
GO:0004483 mRNA (nucleoside-2'-O-)-methyltransferase activity
GO:0005198 structural molecule activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0006410 transcription, RNA-dependent
GO:0019079 viral genome replication
GO:0019028 viral capsid
GO:0019031 viral envelope
GO:0030683 evasion by virus of host immune response
GO:0042025 host cell nucleus
GO:0044167 host cell endoplasmic reticulum membrane
GO:0045449 regulation of transcription
GO:0055036 virion membrane
Regulatory
For research purposes only

Applications of WNV antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1ug/ml
Immunofluorescence 20ug/ml
Western Blotting 1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1666 detects a band of approximately 14kDa in Transfected HeLa cell lysate
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Gould, L.H. & Fikrig, E. (2004) West Nile virus: a growing concern?
    J Clin Invest. 113 (8): 1102-7.
  2. Chu, J.J. et al. (2005) Inhibition of West Nile virus entry by using a recombinant domain III from the envelope glycoprotein.
    J Gen Virol. 86 (Pt 2): 405-12.
  3. Chu, J.J. & Ng, M.L. (2004) Interaction of West Nile virus with alpha v beta 3 integrin mediates virus entry into cells.
    J Biol Chem. 279 (52): 54533-41.
  4. Yang, J.S. et al. (2002) Induction of inflammation by West Nile virus capsid through the caspase-9 apoptotic pathway.
    Emerg Infect Dis. 8 (12): 1379-84.
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