WNV antibody
Rabbit anti West Nile Virus antibody recognizes the ~14 kDa core protein of West Nile Virus (WNV). WNV belongs to the Flaviviridae family which includes closely viruses such as Dengue fever, Yellow fever and Tick borne encephalitis.
West Nile Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope containing envelope and matrix proteins. Research indicates that the viral core protein contributes to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway.
West Nile Virus is spread by mosquitos and is believed to have originated in the West Nile region of Africa but has now spread to Europe, Asia and America where it has become a significant disease affecting human and domestic animal health.
West Nile Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope containing envelope and matrix proteins. Research indicates that the viral core protein contributes to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway.
West Nile Virus is spread by mosquitos and is believed to have originated in the West Nile region of Africa but has now spread to Europe, Asia and America where it has become a significant disease affecting human and domestic animal health.
Product Details
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human West Nile Virus core protein was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A peptide corresponding to an 15 amino acid sequence from near the carboxy terminus of West Nile Virus core protein.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P06935
- Entrez Gene
- WNVgp1
- GO Terms
- GO:0005515 protein binding
- GO:0003724 RNA helicase activity
- GO:0003725 double-stranded RNA binding
- GO:0004252 serine-type endopeptidase activity
- GO:0003968 RNA-directed RNA polymerase activity
- GO:0016021 integral to membrane
- GO:0004482 mRNA (guanine-N7-)-methyltransferase activity
- GO:0004483 mRNA (nucleoside-2'-O-)-methyltransferase activity
- GO:0005198 structural molecule activity
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0006410 transcription, RNA-dependent
- GO:0019079 viral genome replication
- GO:0019028 viral capsid
- GO:0019031 viral envelope
- GO:0030683 evasion by virus of host immune response
- GO:0042025 host cell nucleus
- GO:0044167 host cell endoplasmic reticulum membrane
- GO:0045449 regulation of transcription
- GO:0055036 virion membrane
- Regulatory
- For research purposes only
Applications of WNV antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1ug/ml | ||
| Immunofluorescence | 20ug/ml | ||
| Western Blotting | 1ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1666 detects a band of approximately 14kDa in Transfected HeLa cell lysate
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Gould, L.H. & Fikrig, E. (2004) West Nile virus: a growing concern?
J Clin Invest. 113 (8): 1102-7. -
Chu, J.J. et al. (2005) Inhibition of West Nile virus entry by using a recombinant domain III from the envelope glycoprotein.
J Gen Virol. 86 (Pt 2): 405-12. -
Chu, J.J. & Ng, M.L. (2004) Interaction of West Nile virus with alpha v beta 3 integrin mediates virus entry into cells.
J Biol Chem. 279 (52): 54533-41. -
Yang, J.S. et al. (2002) Induction of inflammation by West Nile virus capsid through the caspase-9 apoptotic pathway.
Emerg Infect Dis. 8 (12): 1379-84.
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