Vaccinia Virus antibody
Rabbit anti vaccinia virus antibody (9503-2057) used to evaluate vaccinia viral protein levels in cell lysates by western blotting.
Image caption:
IAdV and VV can infect and replicate in transformed iPSCs and mitomycin-C treatment inhibits ongoing replication and tumor cell proliferation. Viral protein expression was determined in KP-LC or KPL 160302S cells at 24 and 72 h post-infection ± mitomycin C treatment of cells. Anti-E1A was used to confirm AdV protein expression. Anti-VV coat protein was used to confirm VV protein expression. GAPDH was used as a loading control. **p <0.01 and ***p <0.001.
From: Zhang Z, Lu S, Dunmall LSC, Wang Z, Cheng Z, Zhang Z, Yan W, Chu Y, Gao D, Wang N, Li Y, Wang J, Li Y, Ji Y, Shan D, Li K, Wang P, Dong Y, Dong J, Lemoine NR, Pei D, Zhang L, Wang Y.
Treatment and Prevention of Lung Cancer Using a Virus-Infected Reprogrammed Somatic Cell-Derived Tumor Cell Vaccination (VIReST) Regime.
Front Immunol. 2020 Aug 13;11:1996.
doi: 10.3389/fimmu.2020.01996.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Vaccinia Virus
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Vaccinia Virus
Antibody Quality and Performance Guarantee
| Rabbit anti vaccinia virus antibody recognizes vaccinia virus purified virions. It reacts with Lister and NYCBOH strains of Vaccinia. It does not cross react with Parainfluenza (1-3), RSV, Adenovirus, Influenza A+B, or HSV. |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Vaccinia virus, New York City Board of Health (NYCBOH) strain.
- Approx. Protein Concentrations
- IgG concentration 5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
References for Vaccinia Virus antibody
-
Hornemann, S. et al. (2003) Replication of modified vaccinia virus Ankara in primary chicken embryo fibroblasts requires expression of the interferon resistance gene E3L.
J Virol. 77 (15): 8394-407. -
Vogel, T.U. et al. (2003) Multispecific vaccine-induced mucosal cytotoxic T lymphocytes reduce acute-phase viral replication but fail in long-term control of simian immunodeficiency virus SIVmac239.
J Virol. 77: 13348-60. -
Staib, C. et al. (2004) Construction and isolation of recombinant MVA.
Methods Mol Biol. 269: 77-100. -
Olszewska, W. et al. (2004) Protective and disease-enhancing immune responses induced by recombinant modified vaccinia Ankara (MVA) expressing respiratory syncytial virus proteins.
Vaccine. 23: 215-21. -
Manuel, E.R. et al. (2010) Intergenic region 3 of modified vaccinia ankara is a functional site for insert gene expression and allows for potent antigen-specific immune responses.
Virology. 2010 403: 155-62. -
Buttigieg, K.R. et al. (2014) A Novel Vaccine against Crimean-Congo Haemorrhagic Fever Protects 100% of Animals against Lethal Challenge in a Mouse Model.
PLoS One. 9(3):e91516. -
Kennedy, E.M. et al. (2019) A vaccine based on recombinant modified Vaccinia Ankara containing the nucleoprotein from Lassa virus protects against disease progression in a guinea pig model.
Vaccine. 37 (36): 5404-5413. -
Zhang, Z. et al. (2020) Treatment and Prevention of Lung Cancer Using a Virus-Infected Reprogrammed Somatic Cell-Derived Tumor Cell Vaccination (VIReST) Regime.
Front Immunol. 11: 1996.
View The Latest Product References
- RRID
- AB_619589
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