Rotavirus antibody

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Sheep anti Rotavirus

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: Rotavirus

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Sheep anti Rotavirus antibody recognizes human rotavirus serotypes I, II, III, IV and VIII.

Rotaviruses are RNA viruses from the family Reoviridae that are the the most common cause of gastroenteritis in infants. The nucleic acid is contained in a triple layered protein capsid consisting of outer capsid proteins, VP4 and VP7. Immunization was performed with live Rotavirus serotypes to avoid the risk of surface protein damage (VP7 and VP4).

Target Species: Viral
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Five serotypes of group A Rotavirus strains Wa (serotype I), DS-1 (serotype II), Ito (III), HOCHI (IV) and 69M (VIII).
Approx. Protein Concentrations: IgG concentration 5.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/500	1/5000
Immunofluorescence

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.