Browsing Modes for Business Users

Histone H4 Acetylated antibody

ISO 9001 Quality Assurance Manufacturing | Print
Anti Histone H4 (Acetylated) Antibody thumbnail image 1 Anti Histone H4 (Acetylated) Antibody thumbnail image 2 Anti Histone H4 (Acetylated) Antibody thumbnail image 3
Anti Histone H4 (Acetylated) Antibody gallery image 1
Published customer image:
Rabbit anti acetylated Histone H4 antibody (AHP418) used for the evaluation of acetylated histone H4 expression in rat intestine by western blotting.
Image caption:
Acetylation on histone 4 in the trinitrobenzene sulfonic acid (TNBS) rat model of inflammation. A: Sham (saline treated) operated and TNBS treated rat large intestine. Rats were Sham or TNBS treated for 7 days before sacrifice. Well-advanced inflammation is apparent in the colon of the TNBS rat model. B: Pan acetylation on histone 4 (H4). The Sham model was saline-treated and therefore less inflamed (control). Results were obtained by Western blotting. The ratio of the density of histone H4 bands over β-actin control bands was calculated. In order to evaluate changes in density from different Western blotting experiments control densitometry was denoted as 100% and differences were accounted as increase percentage of the control. Representative examples of bands obtained are also illustrated. Columns represent the densitometric evaluation of three independent experiments (mean ± SEM). (*p <0.05 vs Sham proximal or Sham distal respectively).

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N.
Differential patternsof histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan 27;8(1):1.
Anti Histone H4 (Acetylated) Antibody gallery image 2
Published customer image:
Rabbit anti acetylated Histone H4 antibody (AHP418) used for the evaluation of acetylated histone H4 expression in rat intestine by western blotting.
Image caption:
Acetylation on histones 3 (H3) and 4 (H4) in Lewis and Sprague-Dawley dextran sulfate sodium (5% DSS) treated rats. Tissue samples were obtained from the sigmoid colon of the animals. A: Representative bands of H4 and H3 acetylation as obtained by Western blotting. β-actin levels were measured to ensure equal protein loading. The results are representative of three independent experiments. B, C: Graphical analysis of data Lanes represent: (1) non-DSS treated Lewis rats (control), (2) DSS-treated Lewis rats, (3) non-DSS treated Sprague-Dawley rats (control) (4) DSS-treated Sprague-Dawley rats. Columns represent the mean ± SEM of three independent experiments (*p <0.05). D: Histone 3 (H3) and histone 4 (H4) localisation in Peyer's patches of dextran sulfate sodium (DSS) treated Lewis rats. H3 is acetylated mainly in the mantle zone and H4 is acetylated throughout the surface of Peyer's patches to both mantle zone and germinal centre cells. In Peyer's patches of untreated animals no acetylation on either histone 3 or 4 was apparent. Micrographs are representative of two individual experiments for each strain. Isotype controls show no staining.

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N.
Differential patternsof histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan 27;8(1):1.
Anti Histone H4 (Acetylated) Antibody gallery image 3
Published customer image:
Rabbit anti acetylated Histone H4 antibody (AHP418) used for the evaluation of acetylated histone H4 expression in human Chron's disease intestinal tissue by western blotting.
Image caption:
Acetylation on histone 4 (H4) and H4 lysine residues in Crohn's disease. Columns represent the mean ± SEM of three independent experiments. Four biopsies were pooled to obtain sufficient protein for one experiment (50 μg of protein) (*p <0.05 vs control). Pan acetylation on H4 in Crohn's disease (A). Acetylation on histone 4 (H4) specific lysine residues 5 (K5) (B), K8 (C), K12 (D), and 16 (E), in non-inflamed, inflamed tissue and Peyer's patches of Crohn's disease patients. Results were obtained by Western blotting. Columns represent the mean ± SEM of three independent experiments. (*p <0.05 vs control, #p <0.005 vs non-inflamed CD). Representative images of the bands obtained are illustrated.

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N.
Differential patternsof histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan 27;8(1):1.

Filter by Application:

WB

Rabbit anti Histone H4 (Acetylated)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Histone H4 Acetylated

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
AHP418
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
Ch E IF IP WB 0.1 ml loader
List Price Your Price
loader

Rabbit anti Histone H4 (Acetylated) antibody was raised against the tetra-acetylated form of histone H4 and recognizes acetylated isoforms of histone H4.

Target Species
Synthetic Peptide
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mammals Expected from Sequence
Drosophila
Yeast
Plants Expected from Sequence
Amphibia Expected from Sequence
Human
Arabidopsis
Mouse
Zebrafish
Wheat
Asellus aquaticus
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
KLH-conjugated peptide acetylated at lysines 5,8,12,16: NSGRGAcKGGAcKGLGAcKGGAAcKRHYCC.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Chromatin Immunoprecipitation
ELISA 1/400
Immunofluorescence 1/200
Immunoprecipitation
Western Blotting 1/800
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):HRP
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB * 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

References for Histone H4 Acetylated antibody

  1. Turner, B.M. et al. (1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabeling with site-specific antibodies.
    FEBS Lett. 253 (1-2): 141-5.
  2. Turner, B.M. et al. (1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei.
    Cell. 69 (2): 375-84.
  3. Collas, P. et al. (1999) Active transgenes in zebrafish are enriched in acetylated histone H4 and dynamically associate with RNA Pol II and splicing complexes.
    J Cell Sci. 112: 1045-54.
  4. Tian, L. and Chen, Z.J. (2001) Blocking histone deacetylation in Arabidopsis induces pleiotropic effects on plant gene regulation and development.
    Proc Natl Acad Sci U S A. 98: 200-5.
  5. Lin, X. et al. (2001) Reversal of GSTP1 CpG island hypermethylation and reactivation of pi-class glutathione S-transferase (GSTP1) expression in human prostate cancer cells by treatment with procainamide.
    Cancer Res. 61: 8611-6.
  6. Nye, A.C. et al. (2002) Alteration of large-scale chromatin structure by estrogen receptor.
    Mol Cell Biol. 22: 3437-49.
  7. Santos, A.P. et al. (2002) The architecture of interphase chromosomes and gene positioning are altered by changes in DNA methylation and histone acetylation.
    J Cell Sci. 115: 4597-605.
  8. Perry, P. et al. (2004) A dynamic switch in the replication timing of key regulator genes in embryonic stem cells upon neural induction.
    Cell Cycle. 3 (12): 1645-50.
    9. View The Latest Product References
  9. Catley, M.C. et al. (2004) Inhibitors of protein kinase C (PKC) prevent activated transcription: role of events downstream of NF-kappaB DNA binding.
    J Biol Chem. 279: 18457-66.
  10. Beck, H.C. et al. (2006) Quantitative proteomic analysis of post-translational modifications of human histones.
    Mol Cell Proteomics. 5: 1314-25.
  11. Barzotti, R. et al. (2006) DNA methylation, histone H3 methylation, and histone H4 acetylation in the genome of a crustacean.
    Genome. 49: 87-90.
  12. Foy, R.L. et al. (2008) Role of Jade-1 in the histone acetyltransferase (HAT) HBO1 complex.
    J Biol Chem. 283: 28817-26.
  13. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
    J Inflamm (Lond). 8: 1.
  14. Dincman, T.A. et al. (2016) Histone deacetylase inhibition is cytotoxic to oligodendrocyte precursor cells in vitro and in vivo.
    Int J Dev Neurosci. 54: 53-61.

Further Reading

  1. Belyaev, N. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.
    Hum Genet. 97 (5): 573-8.

Immunofluorescence

Western Blotting

RRID
AB_2116715
UniProt
P62805
Entrez Gene
HIST2H4B
GO Terms
GO:0005515 protein binding
GO:0000723 telomere maintenance
GO:0000786 nucleosome
GO:0003677 DNA binding
GO:0005654 nucleoplasm
GO:0048015 phosphatidylinositol-mediated signaling
GO:0034080 CenH3-containing nucleosome assembly at centromere
GO:0045653 negative regulation of megakaryocyte differentiation

AHP418

147485 155722 159805 166113 169353

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with HISTONE H4 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Synthetic Peptide Products
Please note