Histone H4 Acetylated antibody



Product Details
- Target Species
- Synthetic Peptide
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mammals Expected from Sequence Drosophila Yeast Plants Expected from Sequence Amphibia Expected from Sequence Human Arabidopsis Mouse Zebrafish Wheat Asellus aquaticus - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- KLH-conjugated peptide acetylated at lysines 5,8,12,16: NSGRGAcKGGAcKGLGAcKGGAAcKRHYCC.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P62805
- Entrez Gene
- HIST2H4B
- GO Terms
- GO:0005515 protein binding
- GO:0000723 telomere maintenance
- GO:0000786 nucleosome
- GO:0003677 DNA binding
- GO:0005654 nucleoplasm
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0034080 CenH3-containing nucleosome assembly at centromere
- GO:0045653 negative regulation of megakaryocyte differentiation
- Regulatory
- For research purposes only
Applications of Histone H4 Acetylated antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Chromatin Immunoprecipitation | |||
ELISA | 1/400 | ||
Immunofluorescence | 1/200 | ||
Immunoprecipitation | |||
Western Blotting | 1/800 |
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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|
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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|
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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|
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Histone H4 Acetylated antibody
-
Turner, B.M. et al. (1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabeling with site-specific antibodies.
FEBS Lett. 253 (1-2): 141-5. -
Turner, B.M. et al. (1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei.
Cell. 69 (2): 375-84. -
Belyaev, N. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.
Hum Genet. 97 (5): 573-8. -
Perry, P. et al. (2004) A dynamic switch in the replication timing of key regulator genes in embryonic stem cells upon neural induction.
Cell Cycle. 3 (12): 1645-50. -
Nye, A.C. et al. (2002) Alteration of large-scale chromatin structure by estrogen receptor.
Mol Cell Biol. 22: 3437-49. -
Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 8: 1. -
Tian, L. and Chen, Z.J. (2001) Blocking histone deacetylation in Arabidopsis induces pleiotropic effects on plant gene regulation and development.
Proc Natl Acad Sci U S A. 98: 200-5. -
Beck, H.C. et al. (2006) Quantitative proteomic analysis of post-translational modifications of human histones.
Mol Cell Proteomics. 5: 1314-25. -
Catley, M.C. et al. (2004) Inhibitors of protein kinase C (PKC) prevent activated transcription: role of events downstream of NF-kappaB DNA binding.
J Biol Chem. 279: 18457-66. -
Collas, P. et al. (1999) Active transgenes in zebrafish are enriched in acetylated histone H4 and dynamically associate with RNA Pol II and splicing complexes.
J Cell Sci. 112: 1045-54. -
Foy, R.L. et al. (2008) Role of Jade-1 in the histone acetyltransferase (HAT) HBO1 complex.
J Biol Chem. 283: 28817-26. -
Lin, X. et al. (2001) Reversal of GSTP1 CpG island hypermethylation and reactivation of pi-class glutathione S-transferase (GSTP1) expression in human prostate cancer cells by treatment with procainamide.
Cancer Res. 61: 8611-6. -
Santos, A.P. et al. (2002) The architecture of interphase chromosomes and gene positioning are altered by changes in DNA methylation and histone acetylation.
J Cell Sci. 115: 4597-605. -
Barzotti, R. et al. (2006) DNA methylation, histone H3 methylation, and histone H4 acetylation in the genome of a crustacean.
Genome. 49: 87-90. -
Vega-Flores, G. et al. (2014) The GABAergic septohippocampal pathway is directly involved in internal processes related to operant reward learning.
Cereb Cortex. 24 (8): 2093-107. -
Dincman, T.A. et al. (2016) Histone deacetylase inhibition is cytotoxic to oligodendrocyte precursor cells in vitro and in vivo.
Int J Dev Neurosci. 54: 53-61.
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