IL-1 Beta antibody
Rabbit anti Sheep interleukin 1β antibody (AHP423) used for the detection of bound interleukin 1β from lamb sera in an ELISA using Mouse anti Sheep IL-1&beta antibody, clone 1D4 as a capture antibody (MCA1658). For IL-6, capture is with Mouse anti Sheep IL-6 antibody, clone 4B6 (MCA1659) and detection using Rabbit anti Sheep IL-6 antibody (AHP424). Subsequent visualisation was with HRP conjugated Goat anti Rabbit IgG in both cases.
Image caption:
Systemic and diaphragm cytokine responses. Plasma IL-1β (A) and IL-6 (B) protein content. Values are mean ± SEM. Diaphragm IL-1β (C) and IL-6 (D) mRNA expression. Values are median (with 10th and 90th centiles). * p<0.05 compared to Sal/Sal; # p<0.05 compared to Sal/LPS.
From: Karisnan K, Bakker AJ, Song Y, Noble PB, Pillow JJ, et al. (2015)
Interleukin-1 Receptor Antagonist Protects against Lipopolysaccharide Induced Diaphragm Weakness in Preterm Lambs.
PLoS ONE 10(4): e0124390.
Rabbit anti Ovine interleukin 1β antibody (AHP423) used as a detection antibody in conjunction with Mouse anti Ovine interleukin 1β antibody, clone 1D4 (MCA1658) as a capture reagent in a sandwich ELISA for the evaluation of IL-1β secretion in ovine samples.
Image caption:
(Left) IL-1β secretion in ovine primary astrocyte cultures in response to 6 h LPS exposure without or with pre-incubation with α7nAChR antagonist (B100) or agonist (A10) for 1 h. Single hit, in vitro only LPS exposure; second hit, in vivo systemic and subsequent in vitro LPS exposure 4 to 5 weeks later. Y axis shows fold changes in IL-1β in relation to the levels of sham treatment (LPS). Generalized estimating equations (GEE) modeling results are presented in text and no significance marks are provided in the figure. For both box plots, an asterisk represents an extreme outlier (a value more than 3 times the interquartile range from a quartile). A circle marks outlier with values between 1.5 and 3 box lengths from the upper or lower edge of the box. Briefly, we found significant main term effects (p = 0.019) “treatment” (LPS and α7nAChR drug) as well as main term “hit” (p = 0.010), i.e., the contribution of in vivo LPS exposure, the second hit effect on the IL-1β secretion profile. Results are provided as median {25–75} percentiles. (Right) Identical experimental results from microglia studies are presented for comparison. The main terms “hit” and “treatment” were not significant (p = 0.716 and p = 0.666, respectively), but their interaction was significant (p = 0.026, cf. Figure 1 in (3) where the original results have been published.
From: Cao M, et al..
α7 Nicotinic Acetylcholine Receptor Signaling Modulates Ovine Fetal Brain Astrocytes Transcriptome in Response to Endotoxin.
Front Immunol. 2019 May 9;10:1063.
doi: 10.3389/fimmu.2019.01063.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Sheep interleukin 1β antibody recognizes ovine interleukin-1 beta (IL-1 beta). No cross-reactivity is seen with ovine IL-6, IL-8, MCP-1 or TNF alpha.
Rabbit anti Sheep interleukin 1β antibody neutralizes the bioactivity of ovine IL-1 beta. Removal of sodium azide is recommended prior to use in functional assays.
Rabbit anti Sheep interleukin 1β antibody neutralizes the bioactivity of ovine IL-1 beta. Removal of sodium azide is recommended prior to use in functional assays.
Product Details
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Expected from Sequence Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to ovine IL-1 beta were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Recombinant IL-1 beta.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P21621
- Entrez Gene
- IL-1B
- GO Terms
- GO:0001660 fever generation
- GO:0005125 cytokine activity
- GO:0005149 interleukin-1 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0051781 positive regulation of cell division
- Regulatory
- For research purposes only
Applications of IL-1 Beta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/500 | ||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for IL-1 Beta antibody
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Rothel, J.S. et al. (1997) Analysis of ovine IL-1 beta production in vivo and in vitro by enzyme immunoassay and immunohistochemistry.
Vet Immunol Immunopathol. 57 (3-4): 267-78. -
Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
Clin Diagn Lab Immunol. 11: 463-72. -
Dernfalk, J. et al. (2007) The xMAP technique can be used for detection of the inflammatory cytokines IL-1beta, IL-6 and TNF-alpha in bovine samples.
Vet Immunol Immunopathol. 118: 40-9. -
Moyes, K.M. et al. (2009) Identification of potential markers in blood for the development of subclinical and clinical mastitis in dairy cattle at parturition and during early lactation.
J Dairy Sci. 92: 5419-28. -
Silvestre, F.T. et al. (2011) Effects of differential supplementation of fatty acids during the peripartum and breeding periods of Holstein cows: II. Neutrophil fatty acids and function, and acute phase proteins.
J Dairy Sci. 94 (5): 2285-301. -
Vordermeier, M. et al. (2012) Cytokine responses of Holstein and Sahiwal zebu derived monocytes after mycobacterial infection.
Trop Anim Health Prod. 44: 651-5. -
Xu, A. et al. (2015) The Ovine Fetal and Placental Inflammatory Response to Umbilical Cord Occlusions With Worsening Acidosis.
Reprod Sci. 22 (11): 1409-20. -
Bagu, E.T. et al. (2010) Post-natal changes in testicular concentrations of interleukin-1 alpha and beta and interleukin-6 during sexual maturation in bulls.
Reprod Domest Anim. 45 (2): 336-41. -
Atik, A. et al. (2012) Long-term pulmonary effects of intrauterine exposure to endotoxin following preterm birth in sheep.
Reprod Sci. 19 (12): 1352-64. -
Cortes, M. et al. (2016) RNAseq profiling of primary microglia and astrocyte cultures in near-term ovine fetus: a glial in vivo-in vitro multi-hit paradigm in large mammalian brain
Journal of Neuroscience Methods. Nov 14 [Epub ahead of print] -
Doull, L. et al. (2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
Vet Immunol Immunopathol. 168 (1-2): 97-102. -
Cao, M. et al. (2019) α7 Nicotinic Acetylcholine Receptor Signaling Modulates Ovine Fetal Brain Astrocytes Transcriptome in Response to Endotoxin.
Front Immunol. 10: 1063.
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