IL-1 Beta antibody

Rabbit anti Sheep Interleukin-1 beta

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-1 Beta

Product Code Applications Pack Size List Price Your Price Qty
AHP423
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 ml loader
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loader
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Rabbit anti Sheep interleukin 1β antibody recognizes ovine interleukin-1 beta (IL-1 beta). No cross-reactivity is seen with ovine IL-6, IL-8, MCP-1 or TNF alpha.

Rabbit anti Sheep interleukin 1β antibody neutralizes the bioactivity of ovine IL-1 beta. Removal of sodium azide is recommended prior to use in functional assays.

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat Expected from Sequence
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to ovine IL-1 beta were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Recombinant IL-1 beta.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
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Description Sheep anti Rabbit IgG:FITC
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
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Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
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Description Sheep anti Rabbit IgG:RPE
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
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Description Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
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Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
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Description Goat anti Rabbit IgG (Fc):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
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Description TidyBlot Western Blot Detection Reagent:HRP

References for IL-1 Beta antibody

  1. Rothel, J.S. et al. (1997) Analysis of ovine IL-1 beta production in vivo and in vitro by enzyme immunoassay and immunohistochemistry.
    Vet Immunol Immunopathol. 57 (3-4): 267-78.
  2. Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
    Clin Diagn Lab Immunol. 11: 463-72.
  3. Dernfalk, J. et al. (2007) The xMAP technique can be used for detection of the inflammatory cytokines IL-1beta, IL-6 and TNF-alpha in bovine samples.
    Vet Immunol Immunopathol. 118: 40-9.
  4. Moyes, K.M. et al. (2009) Identification of potential markers in blood for the development of subclinical and clinical mastitis in dairy cattle at parturition and during early lactation.
    J Dairy Sci. 92: 5419-28.
  5. Silvestre, F.T. et al. (2011) Effects of differential supplementation of fatty acids during the peripartum and breeding periods of Holstein cows: II. Neutrophil fatty acids and function, and acute phase proteins.
    J Dairy Sci. 94 (5): 2285-301.
  6. Vordermeier, M. et al. (2012) Cytokine responses of Holstein and Sahiwal zebu derived monocytes after mycobacterial infection.
    Trop Anim Health Prod. 44: 651-5.
  7. Xu, A. et al. (2015) The Ovine Fetal and Placental Inflammatory Response to Umbilical Cord Occlusions With Worsening Acidosis.
    Reprod Sci. 22 (11): 1409-20.
  8. Bagu, E.T. et al. (2010) Post-natal changes in testicular concentrations of interleukin-1 alpha and beta and interleukin-6 during sexual maturation in bulls.
    Reprod Domest Anim. 45 (2): 336-41.
  9. View The Latest Product References
  10. Atik, A. et al. (2012) Long-term pulmonary effects of intrauterine exposure to endotoxin following preterm birth in sheep.
    Reprod Sci. 19 (12): 1352-64.
  11. Cortes, M. et al. (2016) RNAseq profiling of primary microglia and astrocyte cultures in near-term ovine fetus: a glial in vivo-in vitro multi-hit paradigm in large mammalian brain
    Journal of Neuroscience Methods. Nov 14 [Epub ahead of print]
  12. Doull, L. et al. (2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
    Vet Immunol Immunopathol. 168 (1-2): 97-102.
  13. Cao, M. et al. (2019) α7 Nicotinic Acetylcholine Receptor Signaling Modulates Ovine Fetal Brain Astrocytes Transcriptome in Response to Endotoxin.
    Front Immunol. 10: 1063.
  14. Damiano, S. et al. (2022) Red orange and lemon extract preserve from oxidative stress, DNA damage and inflammatory status in lambs
    It J Anim Sci. 21 (1): 934-42.

ELISA

RRID
AB_322127
UniProt
P21621
Entrez Gene
IL-1B
GO Terms
GO:0001660 fever generation
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0051781 positive regulation of cell division
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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