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IL-1 Beta antibody

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Anti Sheep Interleukin-1 beta Antibody thumbnail image 1 Anti Sheep Interleukin-1 beta Antibody thumbnail image 2
Anti Sheep Interleukin-1 beta Antibody gallery image 1
Published customer image:
Rabbit anti Sheep interleukin 1β antibody (AHP423) used for the detection of bound interleukin 1β from lamb sera in an ELISA using Mouse anti Sheep IL-1&beta antibody, clone 1D4 as a capture antibody (MCA1658). For IL-6, capture is with Mouse anti Sheep IL-6 antibody, clone 4B6 (MCA1659) and detection using Rabbit anti Sheep IL-6 antibody (AHP424). Subsequent visualisation was with HRP conjugated Goat anti Rabbit IgG in both cases.
Image caption:
Systemic and diaphragm cytokine responses. Plasma IL-1β (A) and IL-6 (B) protein content. Values are mean ± SEM. Diaphragm IL-1β (C) and IL-6 (D) mRNA expression. Values are median (with 10th and 90th centiles). * p<0.05 compared to Sal/Sal; # p<0.05 compared to Sal/LPS.

From: Karisnan K, Bakker AJ, Song Y, Noble PB, Pillow JJ, et al. (2015)
Interleukin-1 Receptor Antagonist Protects against Lipopolysaccharide Induced Diaphragm Weakness in Preterm Lambs.
PLoS ONE 10(4): e0124390.
Anti Sheep Interleukin-1 beta Antibody gallery image 2
Published customer image:
Rabbit anti Ovine interleukin 1β antibody (AHP423) used as a detection antibody in conjunction with Mouse anti Ovine interleukin 1β antibody, clone 1D4 (MCA1658) as a capture reagent in a sandwich ELISA for the evaluation of IL-1β secretion in ovine samples.
Image caption:
(Left) IL-1β secretion in ovine primary astrocyte cultures in response to 6 h LPS exposure without or with pre-incubation with α7nAChR antagonist (B100) or agonist (A10) for 1 h. Single hit, in vitro only LPS exposure; second hit, in vivo systemic and subsequent in vitro LPS exposure 4 to 5 weeks later. Y axis shows fold changes in IL-1β in relation to the levels of sham treatment (LPS). Generalized estimating equations (GEE) modeling results are presented in text and no significance marks are provided in the figure. For both box plots, an asterisk represents an extreme outlier (a value more than 3 times the interquartile range from a quartile). A circle marks outlier with values between 1.5 and 3 box lengths from the upper or lower edge of the box. Briefly, we found significant main term effects (p = 0.019) “treatment” (LPS and α7nAChR drug) as well as main term “hit” (p = 0.010), i.e., the contribution of in vivo LPS exposure, the second hit effect on the IL-1β secretion profile. Results are provided as median {25–75} percentiles. (Right) Identical experimental results from microglia studies are presented for comparison. The main terms “hit” and “treatment” were not significant (p = 0.716 and p = 0.666, respectively), but their interaction was significant (p = 0.026, cf. Figure 1 in (3) where the original results have been published.

From: Cao M, et al..
α7 Nicotinic Acetylcholine Receptor Signaling Modulates Ovine Fetal Brain Astrocytes Transcriptome in Response to Endotoxin.
Front Immunol. 2019 May 9;10:1063.
doi: 10.3389/fimmu.2019.01063.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Rabbit anti Sheep Interleukin-1 beta

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP423
Datasheet
E WB
MSDS
0.1 ml loader

Rabbit anti Sheep interleukin 1β antibody recognizes ovine interleukin-1 beta (IL-1 beta). No cross-reactivity is seen with ovine IL-6, IL-8, MCP-1 or TNF alpha.

Rabbit anti Sheep interleukin 1β antibody neutralizes the bioactivity of ovine IL-1 beta. Removal of sodium azide is recommended prior to use in functional assays.

Product Details

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat Expected from Sequence
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to ovine IL-1 beta were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant IL-1 beta.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P21621
Entrez Gene
IL-1B
GO Terms
GO:0001660 fever generation
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0051781 positive regulation of cell division
Regulatory
For research purposes only

Applications of IL-1 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Application Based External Images

ELISA

Product Specific References

References for IL-1 Beta antibody

  1. Rothel, J.S. et al. (1997) Analysis of ovine IL-1 beta production in vivo and in vitro by enzyme immunoassay and immunohistochemistry.
    Vet Immunol Immunopathol. 57 (3-4): 267-78.
  2. Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
    Clin Diagn Lab Immunol. 11: 463-72.
  3. Dernfalk, J. et al. (2007) The xMAP technique can be used for detection of the inflammatory cytokines IL-1beta, IL-6 and TNF-alpha in bovine samples.
    Vet Immunol Immunopathol. 118: 40-9.
  4. Moyes, K.M. et al. (2009) Identification of potential markers in blood for the development of subclinical and clinical mastitis in dairy cattle at parturition and during early lactation.
    J Dairy Sci. 92: 5419-28.
  5. Silvestre, F.T. et al. (2011) Effects of differential supplementation of fatty acids during the peripartum and breeding periods of Holstein cows: II. Neutrophil fatty acids and function, and acute phase proteins.
    J Dairy Sci. 94 (5): 2285-301.
  6. Vordermeier, M. et al. (2012) Cytokine responses of Holstein and Sahiwal zebu derived monocytes after mycobacterial infection.
    Trop Anim Health Prod. 44: 651-5.
  7. Xu, A. et al. (2015) The Ovine Fetal and Placental Inflammatory Response to Umbilical Cord Occlusions With Worsening Acidosis.
    Reprod Sci. 22 (11): 1409-20.
  8. Bagu, E.T. et al. (2010) Post-natal changes in testicular concentrations of interleukin-1 alpha and beta and interleukin-6 during sexual maturation in bulls.
    Reprod Domest Anim. 45 (2): 336-41.
  9. Atik, A. et al. (2012) Long-term pulmonary effects of intrauterine exposure to endotoxin following preterm birth in sheep.
    Reprod Sci. 19 (12): 1352-64.
  10. Cortes, M. et al. (2016) RNAseq profiling of primary microglia and astrocyte cultures in near-term ovine fetus: a glial in vivo-in vitro multi-hit paradigm in large mammalian brain
    Journal of Neuroscience Methods. Nov 14 [Epub ahead of print]
  11. Doull, L. et al. (2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
    Vet Immunol Immunopathol. 168 (1-2): 97-102.
  12. Cao, M. et al. (2019) α7 Nicotinic Acetylcholine Receptor Signaling Modulates Ovine Fetal Brain Astrocytes Transcriptome in Response to Endotoxin.
    Front Immunol. 10: 1063.
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