IgG antibody

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Rabbit anti Sheep IgG Ab (H/L):HRP thumbnail image 1

Rabbit anti Sheep IgG Ab (H/L):HRP thumbnail image 2

Rabbit anti Sheep IgG Ab (H/L):HRP thumbnail image 3

Rabbit anti Sheep IgG Ab (H/L):HRP gallery image 1 — **Published customer image:**
Horseradish peroxidase conjugated Rabbit anti Sheep IgG antibody (**5184-2504**) used for the detection of sheep IgG in serum following *Toxoplasma gondii* infection by ELISA.
**Image caption:**
IgG response of the sheep in TLA ELISA in function of time.

From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E. Parasite distribution and associated immune response during the acute phase of *Toxoplasma gondii* infection in sheep.
BMC Vet Res. 2014 Dec 16;10:293.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Rabbit anti Sheep IgG Ab (H/L):HRP gallery image 2 — **Published customer image:**
Horseradish peroxidase conjugated Rabbit anti Sheep IgG antibody (**5184-2504**) used for the detection of bound Sheep anti NFκB antibody (VPA00015) by western blotting of HEK293 cell lysates.
**Image caption:**
NF-κB p65 protein expression levels (a) Representative immunoblot membrane; (b) Combined densitometry data of three individual experiments, indicating fold changes in the protein expression of NF-κB p65, normalized to the total proteins transferred onto the membrane. BSA: bovine serum albumin; AGEs: advanced glycation end products; * p < 0.05; ** p < 0.01.

From: Serban AI, Stanca L, Geicu OI, Dinischiotu A. AGEs-Induced IL-6 Synthesis Precedes RAGE Up-Regulation in HEK 293 Cells: An Alternative Inflammatory Mechanism?
Int J Mol Sci. 2015 Aug 25;16(9):20100-17.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Rabbit anti Sheep IgG Ab (H/L):HRP gallery image 3 — **Published customer image:**
Horseradish peroxidase conjugated Rabbit anti Sheep IgG antibody (**5184-2504**) used for the detection of bound Sheep anti NFκB antibody (VPA00015) in normal human skin derived fibroblasts by western blotting.
**Image caption:**
AGEs induced signaling in CCD 1070SK fibroblasts. p-ERK1/2 and NF-κB p65 protein expression levels (a) Representative immunoblot membranes; (b) Combined densitometry data indicating fold changes in the protein expression of p-ERK1/2 (b) and NF-κB p65 (c). Immunoreactive bands were normalized to the total proteins transferred onto the membrane in the corresponding lane. Data are relative to BSA and nonimmune IgG treated cells. BSA: bovine serum albumin; AGEs: advanced glycation end products; * p < 0.05; ** p < 0.01; *** p < 0.001.

From: Serban AI, Stanca L, Geicu OI, Munteanu MC, Dinischiotu A (2016)
RAGE and TGF-β1 Cross-Talk Regulate Extracellular Matrix Turnover and Cytokine Synthesis in AGEs Exposed Fibroblast Cells.
PLoS ONE 11(3): e0152376.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Rabbit anti Sheep IgG (H/L):HRP

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
5184-2504 Datasheet	C E WB SDS	2 mg	Log in		Get Quote

Rabbit anti Sheep IgG antibody recognizes both the light and heavy chains of sheep IgG.

Product Details

Target Species: Sheep
Product Form: Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
Reconstitution: Reconstitute with 1ml of sterile distilled water. For extended storage use 50% glycerol instead of water.
Antiserum Preparation: Antiserum to sheep IgG was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.01% Gentamicin Sulfate
1% Bovine Serum Albumin
Immunogen: Sheep IgG whole molecule.

Storage Information

Storage: This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

Regulatory: For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/8000	1/40000
Immunohistology - Frozen	1/500	1/2500
Western Blotting	1/1000	1/5000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
AbGUARD® HRP Stabilizer Plus	BUF052A	C E P WB	100 ml	Log in	Get Quote
AbGUARD® HRP Stabilizer Plus	BUF052B	C E P WB	500 ml	Log in	Get Quote
AbGUARD® HRP Stabilizer Plus	BUF052C	C E P WB	1000 ml	Log in	Get Quote
TMB Signal+	BUF054A	E	100 ml	Log in	Get Quote
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Application Based External Images

ELISA

Product Specific References

References for IgG antibody

Vande Walle, K. et al. (2011) Rectal inoculation of sheep with E. coli O157:H7 results in persistent infection in the absence of a protective immune response.
Vet Microbiol. 147 (3-4): 376-82.
Maronde, E. et al. (2011) Dynamics in enzymatic protein complexes offer a novel principle for the regulation of melatonin synthesis in the human pineal gland.
J Pineal Res. 51: 145-55.
Balan, P. et al. (2009) Orally administered ovine serum immunoglobulins influence growth performance, organ weights, and gut morphology in growing rats.
J Nutr. 139: 244-9.
Balan, P. et al. (2014) Recovery of intact IgG in the gastrointestinal tract of the growing rat following ingestion of an ovine serum immunoglobulin.
J Anim Physiol Anim Nutr (Berl). 98 (2): 209-14.
Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
BMC Vet Res. 10: 293.
Serban, A.I. et al. (2015) AGEs-Induced IL-6 Synthesis Precedes RAGE Up-Regulation in HEK 293 Cells: An Alternative Inflammatory Mechanism?
Int J Mol Sci. 16 (9): 20100-17.
Serban AI et al. (2016) RAGE and TGF-β1 Cross-Talk Regulate Extracellular Matrix Turnover and Cytokine Synthesis in AGEs Exposed Fibroblast Cells.
PLoS One. 11 (3): e0152376.

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