IgG antibody



Product Details
- Target Species
- Sheep
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
- Reconstitution
- Reconstitute with 1ml of sterile distilled water. For extended storage use 50% glycerol instead of water.
- Antiserum Preparation
- Antiserum to sheep IgG was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Gentamicin Sulfate
1% Bovine Serum Albumin - Immunogen
- Sheep IgG whole molecule.
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgG antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/8000 | 1/40000 | |
Immunohistology - Frozen | 1/500 | 1/2500 | |
Western Blotting | 1/1000 | 1/5000 |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TMB Signal+ | BUF054A | E | 100 ml | ||
TMB Core | BUF056A | E | 100 ml | ||
TMB Core+ | BUF062A | E | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml |
Product Specific References
References for IgG antibody
-
Vande Walle, K. et al. (2011) Rectal inoculation of sheep with E. coli O157:H7 results in persistent infection in the absence of a protective immune response.
Vet Microbiol. 147 (3-4): 376-82. -
Maronde, E. et al. (2011) Dynamics in enzymatic protein complexes offer a novel principle for the regulation of melatonin synthesis in the human pineal gland.
J Pineal Res. 51: 145-55. -
Balan, P. et al. (2009) Orally administered ovine serum immunoglobulins influence growth performance, organ weights, and gut morphology in growing rats.
J Nutr. 139: 244-9. -
Balan, P. et al. (2014) Recovery of intact IgG in the gastrointestinal tract of the growing rat following ingestion of an ovine serum immunoglobulin.
J Anim Physiol Anim Nutr (Berl). 98 (2): 209-14. -
Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
BMC Vet Res. 10: 293. -
Serban, A.I. et al. (2015) AGEs-Induced IL-6 Synthesis Precedes RAGE Up-Regulation in HEK 293 Cells: An Alternative Inflammatory Mechanism?
Int J Mol Sci. 16 (9): 20100-17. -
Serban AI et al. (2016) RAGE and TGF-β1 Cross-Talk Regulate Extracellular Matrix Turnover and Cytokine Synthesis in AGEs Exposed Fibroblast Cells.
PLoS One. 11 (3): e0152376.
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