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IgG antibody

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Rabbit anti Sheep IgG Ab (H/L):HRP thumbnail image 1 Rabbit anti Sheep IgG Ab (H/L):HRP thumbnail image 2 Rabbit anti Sheep IgG Ab (H/L):HRP thumbnail image 3
Rabbit anti Sheep IgG Ab (H/L):HRP gallery image 1
Published customer image:
Horseradish peroxidase conjugated Rabbit anti Sheep IgG antibody (5184-2504) used for the detection of sheep IgG in serum following Toxoplasma gondii infection by ELISA.
Image caption:
IgG response of the sheep in TLA ELISA in function of time.

From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E. Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
BMC Vet Res. 2014 Dec 16;10:293.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Sheep IgG Ab (H/L):HRP gallery image 2
Published customer image:
Horseradish peroxidase conjugated Rabbit anti Sheep IgG antibody (5184-2504) used for the detection of bound Sheep anti NFκB antibody (VPA00015) by western blotting of HEK293 cell lysates.
Image caption:
NF-κB p65 protein expression levels (a) Representative immunoblot membrane; (b) Combined densitometry data of three individual experiments, indicating fold changes in the protein expression of NF-κB p65, normalized to the total proteins transferred onto the membrane. BSA: bovine serum albumin; AGEs: advanced glycation end products; * p < 0.05; ** p < 0.01.

From: Serban AI, Stanca L, Geicu OI, Dinischiotu A. AGEs-Induced IL-6 Synthesis Precedes RAGE Up-Regulation in HEK 293 Cells: An Alternative Inflammatory Mechanism?
Int J Mol Sci. 2015 Aug 25;16(9):20100-17.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Sheep IgG Ab (H/L):HRP gallery image 3
Published customer image:
Horseradish peroxidase conjugated Rabbit anti Sheep IgG antibody (5184-2504) used for the detection of bound Sheep anti NFκB antibody (VPA00015) in normal human skin derived fibroblasts by western blotting.
Image caption:
AGEs induced signaling in CCD 1070SK fibroblasts. p-ERK1/2 and NF-κB p65 protein expression levels (a) Representative immunoblot membranes; (b) Combined densitometry data indicating fold changes in the protein expression of p-ERK1/2 (b) and NF-κB p65 (c). Immunoreactive bands were normalized to the total proteins transferred onto the membrane in the corresponding lane. Data are relative to BSA and nonimmune IgG treated cells. BSA: bovine serum albumin; AGEs: advanced glycation end products; * p < 0.05; ** p < 0.01; *** p < 0.001.

From: Serban AI, Stanca L, Geicu OI, Munteanu MC, Dinischiotu A (2016)
RAGE and TGF-β1 Cross-Talk Regulate Extracellular Matrix Turnover and Cytokine Synthesis in AGEs Exposed Fibroblast Cells.
PLoS ONE 11(3): e0152376.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Rabbit anti Sheep IgG (H/L):HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
5184-2504
Datasheet
C E WB
SDS
2 mg loader

Rabbit anti Sheep IgG antibody recognizes both the light and heavy chains of sheep IgG.

Product Details

Target Species
Sheep
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
Reconstitution
Reconstitute with 1ml of sterile distilled water. For extended storage use 50% glycerol instead of water.
Antiserum Preparation
Antiserum to sheep IgG was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Gentamicin Sulfate
1% Bovine Serum Albumin
Immunogen
Sheep IgG whole molecule.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/8000 1/40000
Immunohistology - Frozen 1/500 1/2500
Western Blotting 1/1000 1/5000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml

Application Based External Images

ELISA

Product Specific References

References for IgG antibody

  1. Vande Walle, K. et al. (2011) Rectal inoculation of sheep with E. coli O157:H7 results in persistent infection in the absence of a protective immune response.
    Vet Microbiol. 147 (3-4): 376-82.
  2. Maronde, E. et al. (2011) Dynamics in enzymatic protein complexes offer a novel principle for the regulation of melatonin synthesis in the human pineal gland.
    J Pineal Res. 51: 145-55.
  3. Balan, P. et al. (2009) Orally administered ovine serum immunoglobulins influence growth performance, organ weights, and gut morphology in growing rats.
    J Nutr. 139: 244-9.
  4. Balan, P. et al. (2014) Recovery of intact IgG in the gastrointestinal tract of the growing rat following ingestion of an ovine serum immunoglobulin.
    J Anim Physiol Anim Nutr (Berl). 98 (2): 209-14.
  5. Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
    BMC Vet Res. 10: 293.
  6. Serban, A.I. et al. (2015) AGEs-Induced IL-6 Synthesis Precedes RAGE Up-Regulation in HEK 293 Cells: An Alternative Inflammatory Mechanism?
    Int J Mol Sci. 16 (9): 20100-17.
  7. Serban AI et al. (2016) RAGE and TGF-β1 Cross-Talk Regulate Extracellular Matrix Turnover and Cytokine Synthesis in AGEs Exposed Fibroblast Cells.
    PLoS One. 11 (3): e0152376.

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