Rabbit anti Sheep IgG antibody recognizes both the light and heavy chains of sheep IgG.
- Target Species
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilized
- Reconstitute with 1ml of sterile distilled water. For extended storage use 50% glycerol instead of water.
- Antiserum Preparation
- Antiserum to sheep IgG was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Gentamicin Sulfate
1% Bovine Serum Albumin
- Sheep IgG whole molecule.
- This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IgG antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Useful Reagents Available
Application Based External Images
Product Specific References
References for IgG antibody
Vande Walle, K. et al. (2011) Rectal inoculation of sheep with E. coli O157:H7 results in persistent infection in the absence of a protective immune response.
Vet Microbiol. 147 (3-4): 376-82.
Maronde, E. et al. (2011) Dynamics in enzymatic protein complexes offer a novel principle for the regulation of melatonin synthesis in the human pineal gland.
J Pineal Res. 51: 145-55.
Balan, P. et al. (2009) Orally administered ovine serum immunoglobulins influence growth performance, organ weights, and gut morphology in growing rats.
J Nutr. 139: 244-9.
Balan, P. et al. (2014) Recovery of intact IgG in the gastrointestinal tract of the growing rat following ingestion of an ovine serum immunoglobulin.
J Anim Physiol Anim Nutr (Berl). 98 (2): 209-14.
Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
BMC Vet Res. 10: 293.
Serban, A.I. et al. (2015) AGEs-Induced IL-6 Synthesis Precedes RAGE Up-Regulation in HEK 293 Cells: An Alternative Inflammatory Mechanism?
Int J Mol Sci. 16 (9): 20100-17.
Serban AI et al. (2016) RAGE and TGF-β1 Cross-Talk Regulate Extracellular Matrix Turnover and Cytokine Synthesis in AGEs Exposed Fibroblast Cells.
PLoS One. 11 (3): e0152376.
Loureiro, D. et al. (2017) Seroprevalence of antibodies against bacterial pathogens in sheep from Equatorial Guinea.
Rev Sci Tech. 36 (3): 965-70.
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