|Biotinylated Rabbit anti Sheep IgG polyclonal antibody recognizes intact ovine IgG, raised against the whole IgG molecule it may recognize epitpoes encompassing both heavy and light chains. The antibody has not been cross adsorbed and may react with immunoglobulins of other subtypes and from other species.
Biotinylated Rabbit anti Sheep IgG polyclonal antibody is a useful and robust reagent for the detection of bound sheep antibodies with labeled streptavidin as a tertiary reagent.
Biotinylated Rabbit anti Sheep IgG polyclonal antibody has been used successfully for evaluation of IgG responses in vaccinated and challenged sheep (Ramos et al. 2018).
- Target Species
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Reconstitute in 1.5 ml sterile distilled water.
- Purified IgG prepared by immunoaffinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.05% Sodium Azide (NaN3) 1.5% Bovine Serum Albumin
- Whole sheep IgG.
- For research purposes only
- 12 months from date of despatch
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/500||1/5,000|
References for IgG antibody
Ramos, A. et al. (2018) Melatonin enhances responsiveness to Dichelobacter nodosus. vaccine in sheep and increases peripheral blood CD4 T lymphocytes and IgG-expressing B lymphocytes.
Vet Immunol Immunopathol. 206: 1-8.
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