Tyrosine Hydroxylase (pSer40) antibody

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Rabbit anti Tyrosine Hydroxylase (pSer40)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP912
Datasheet
C IF WB
MSDS
0.1 ml loader

Rabbit anti Rat tyrosine hydroxylase (pSer40) antibody recognizes tyrosine hydroxylase (TH), also known as tyrosine 3-monooxygenase, when phosphorylated at serine 40.

Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines dopamine (DA), norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain.

Phosphorylation of TH has been shown to occur at several serine residues. Phosphorylation at serine 40 results in an increase in hydroxylase activity, and phosphorylation at serine 19 is reported to promote phosphorylation of the serine 40 residue (Dunkley et al. 2004).

Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson’s disease (Pearson et al 1979).

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Human Expected from Sequence
Pig Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to phosphorylated tyrosine hydroxylase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5
Preservative Stabilisers
0.09%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence within Tyrosine Hydroxylase which includes the phosphorylated residue Ser 40.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch.

More Information

UniProt
P07101 Related reagents
Entrez Gene
TH Related reagents
GO Terms
GO:0005515 protein binding
GO:0042136 neurotransmitter biosynthetic process
GO:0001666 response to hypoxia
GO:0001963 synaptic transmission, dopaminergic
GO:0003007 heart morphogenesis
GO:0004511 tyrosine 3-monooxygenase activity
GO:0005829 cytosol
GO:0005634 nucleus
GO:0005790 smooth endoplasmic reticulum
GO:0055114 oxidation-reduction process
GO:0006585 dopamine biosynthetic process from tyrosine
GO:0007269 neurotransmitter secretion
GO:0007601 visual perception
GO:0007612 learning
GO:0007613 memory
GO:0007626 locomotory behavior
GO:0008016 regulation of heart contraction
GO:0009898 internal side of plasma membrane
GO:0033162 melanosome membrane
GO:0042418 epinephrine biosynthetic process
GO:0042421 norepinephrine biosynthetic process
GO:0042446 hormone biosynthetic process
GO:0042462 eye photoreceptor cell development
GO:0043204 perikaryon
GO:0043473 pigmentation
GO:0045471 response to ethanol
GO:0048596 embryonic camera-type eye morphogenesis
Regulatory
For research purposes only

Applications of Tyrosine Hydroxylase (pSer40) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/1000
Immunohistology - Frozen 1/1000
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP912 detects a band of approximately 60kDa in PC-12 cell lysates, following stimulation by Okadaic acid.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Application Based External Images

Immunofluorescence

Product Specific References

References for Tyrosine Hydroxylase (pSer40) antibody

  1. Xiao, M.F. et al. (2009) Neural cell adhesion molecule modulates dopaminergic signaling and behavior by regulating dopamine D2 receptor internalization.
    J Neurosci. 29 (47): 14752-63.
  2. Haycock, J.W. (1990) Phosphorylation of tyrosine hydroxylase in situ at serine 8, 19, 31, and 40.
    J Biol Chem. 265 (20): 11682-91.
  3. Hoard, J.L. et al. (2008) Cholinergic neurons of mouse intrinsic cardiac ganglia contain noradrenergic enzymes, norepinephrine transporters, and the neurotrophin receptors tropomyosin-related kinase A and p75.
    Neuroscience. 156 (1): 129-42.
  4. Li, S. et al. (2013) The neural cell adhesion molecule (NCAM) associates with and signals through p21-activated kinase 1 (Pak1).
    J Neurosci. 33 (2): 790-803.

Further Reading

  1. Bevilaqua, L.R. et al. (2001) Phosphorylation of Ser(19) alters the conformation of tyrosine hydroxylase to increase the rate of phosphorylation of Ser(40).
    J Biol Chem. 276 (44): 40411-6.