RANTES antibody

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Rabbit anti Rat RANTES:Biotin

Rabbit anti Rat RANTES

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAR18B E WB 50 µg
AAR18G E WB 0.1 mg
Rabbit anti Rat RANTES antibody recognizes rat RANTES, also known as CCL5, a chemoattractant for blood monocytes, memory T-helper cells and eosinophils. RANTES causes the release of histamine from basophils and activates eosinophils.

Rabbit anti Rat RANTES antibody has not been cross-adsorbed and may react with homologous peptides from related species.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 1.0 ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antisera to rat RANTES were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by antigen affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None Present
Preservative Stabilisers
None present.
Immunogen
Recombinant rat RANTES
Approx. Protein Concentrations
IgG concentration 50 ug/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. The addition of 0.09% sodium azide is recommended for long term storage.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P50231 Related reagents
Entrez Gene
Ccl5 Related reagents
GO Terms
GO:0002544 chronic inflammatory response
GO:0009615 response to virus
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006955 immune response
GO:0007568 aging
GO:0008009 chemokine activity
GO:0008201 heparin binding
GO:0042493 response to drug
GO:0009651 response to salt stress
GO:0010628 positive regulation of gene expression
GO:0014823 response to activity
GO:0018894 dibenzo-p-dioxin metabolic process
GO:0031622 positive regulation of fever generation
GO:0032868 response to insulin stimulus
GO:0043526 neuroprotection
GO:0043627 response to estrogen stimulus
GO:0045666 positive regulation of neuron differentiation
GO:0045672 positive regulation of osteoclast differentiation
GO:0045766 positive regulation of angiogenesis
GO:0046470 phosphatidylcholine metabolic process
GO:0048247 lymphocyte chemotaxis
GO:0050667 homocysteine metabolic process
GO:0051384 response to glucocorticoid stimulus
GO:0060548 negative regulation of cell death
GO:0060754 positive regulation of mast cell chemotaxis
GO:0070723 response to cholesterol
GO:0071222 cellular response to lipopolysaccharide
GO:0071307 cellular response to vitamin K
GO:0071315 cellular response to morphine
GO:0071356 cellular response to tumor necrosis factor
GO:0071361 cellular response to ethanol
GO:0071403 cellular response to high density lipoprotein particle stimulus
GO:0071445 cellular response to protein stimulus
GO:0071448 cellular response to alkyl hydroperoxide
GO:0071560 cellular response to transforming growth factor beta stimulus
Regulatory
For research purposes only

Applications of RANTES antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.15ug/ml 0.30ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
ELISA 0.5ug/ml 2.0ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This antibody may be used in and indirect ELISA or as a detection reagent in a sandwich ELISA in combination with AAR18G as a capture reagent, and recombinant rat RANTES (PRP18) as a standard.
ELISA
This antibody may be used in and indirect ELISA or as a capture reagent in a sandwich ELISA in combination with AAR18B as a detection reagent, and recombinant rat RANTES (PRP18) as a standard.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*