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Nmdar NR2B (pTyr1252) antibody

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Western blot analysis of rat hippocampal lysate showing phosphospecific immunolabeling of the ~180 kDa NR2B subunit of the NMDR phosphorylated at Tyr1252 using Rabbit anti NMDA receptor antibody (pTyr1252) antibody (AHP1235).

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Rabbit anti NMDA Receptor NR2B (pTyr1252)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Nmdar NR2B (pTyr1252)

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Product Code Applications Pack Size List Price Your Price Qty
AHP1235
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 ml loader
List Price Your Price
loader

Rabbit anti Rat NMDA receptor NR2B (pTyr1252) antibody recognizes NMDA receptor NR2B, also known as glutamate receptor ionotropic, NMDA 2B (GluN2B), glutamate [NMDA] receptor subunit epsilon-2 and N-methyl D-aspartate receptor subtype 2B (NMDAR2B), when phosphorylated at tyrosine 1252.

Receptors for NMDA belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Several antagonists and agonists of NMDA receptors (NMDAR) have been identified, including the glutamate analogue Homoquinolinic acid, which displays a higher affinity for NR2B-containing NMDAR. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+ permeability.

The involvement of NMDAR in the CNS implicated in neurodegenerative diseases such as Alzheimer's (Popke 2003) and also epilepsy and ischemic neuronal cell death.

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human Expected from Sequence
Mouse Expected from Sequence
Dog Expected from Sequence
Bovine Expected from Sequence
Chicken Expected from Sequence
Zebrafish Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to rat NMDAR NR2B subunit pTyr1252 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10 mM Hepes pH7.5
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
50% Glycerol
0.01%Bovine Serum Albumin
Immunogen
Phosphopeptide corresponding to smino acid residues surrounding the phosphorylated tyrosine 1252 of the NR2B subunit of rat NMDA receptor.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1235 detects a band/s of approximately 180kDa in rat hippocampal cell lysates.

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References for Nmdar NR2B (pTyr1252) antibody

  1. Ishii, T. et al. (1993) Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits.
    J Biol Chem. 268 (4): 2836-43.
  2. Takasu, M.A. et al. (2002) Modulation of NMDA receptor-dependent calcium influx and gene expression through EphB receptors.
    Science. 295 (5554): 491-5.
  3. Rosenblum, K. et al. (1996) Long-term potentiation increases tyrosine phosphorylation of the N-methyl-D-aspartate receptor subunit 2B in rat dentate gyrus in vivo.
    Proc Natl Acad Sci U S A. 93 (19): 10457-60.
  4. Popke, E.J. (2003) From anticholinesterase toxicity to Alzheimer's disease: important interactions of cholinergic and NMDA receptor systems.
    Toxicol Sci. 72 (2): 185-7.

RRID
AB_877491
UniProt
Q00960
Entrez Gene
Grin2b
GO Terms
GO:0001508 regulation of action potential
GO:0001975 response to amphetamine
GO:0050839 cell adhesion molecule binding
GO:0004972 N-methyl-D-aspartate selective glutamate receptor activity
GO:0005234 extracellular-glutamate-gated ion channel activity
GO:0007616 long-term memory
GO:0008013 beta-catenin binding
GO:0008144 drug binding
GO:0008270 zinc ion binding
GO:0008306 associative learning
GO:0051707 response to other organism
GO:0009743 response to carbohydrate stimulus
GO:0010350 cellular response to magnesium starvation
GO:0010942 positive regulation of cell death
GO:0014049 positive regulation of glutamate secretion
GO:0014069 postsynaptic density
GO:0016600 flotillin complex
GO:0017146 N-methyl-D-aspartate selective glutamate receptor complex
GO:0021766 hippocampus development
GO:0021987 cerebral cortex development
GO:0030018 Z disc
GO:0030054 cell junction
GO:0030288 outer membrane-bounded periplasmic space
GO:0031749 D2 dopamine receptor binding
GO:0032026 response to magnesium ion
GO:0034097 response to cytokine stimulus
GO:0035235 ionotropic glutamate receptor signaling pathway
GO:0035255 ionotropic glutamate receptor binding
GO:0042165 neurotransmitter binding
GO:0042220 response to cocaine
GO:0042734 presynaptic membrane
GO:0043083 synaptic cleft
GO:0043113 receptor clustering
GO:0043195 terminal button
GO:0043197 dendritic spine
GO:0043408 regulation of MAPKKK cascade
GO:0045211 postsynaptic membrane
GO:0045471 response to ethanol
GO:0046982 protein heterodimerization activity
GO:0048169 regulation of long-term neuronal synaptic plasticity
GO:0048511 rhythmic process
GO:0050806 positive regulation of synaptic transmission
GO:0051592 response to calcium ion
GO:0051597 response to methylmercury
GO:0051602 response to electrical stimulus
GO:0060416 response to growth hormone stimulus
GO:0060992 response to fungicide
GO:0071230 cellular response to amino acid stimulus
GO:0071287 cellular response to manganese ion
GO:0071359 cellular response to dsRNA
GO:0071363 cellular response to growth factor stimulus
GO:0071396 cellular response to lipid
GO:0071407 cellular response to organic cyclic compound
GO:0071445 cellular response to protein stimulus

AHP1235

160314

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