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Nmdar NR2B antibody

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Rabbit anti Rat NMDA Receptor NR2B

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
6870-1109
Datasheet
C IP WB
SDS
10 µg loader

Rabbit anti Rat NMDA Receptor NR2B antibody recognizes the ~180 kDa NMDA (N-methyl-D-aspartate) receptor NMDAR2B subunit.

NMDA receptors are a class of ionotropic glutamate receptors. NMDA channels have been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), or NMDAR2D (GRIN2D).

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Human
Fish
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 50µl of Phosphate buffered saline
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Ammonium bicarbonate
Preservative Stabilisers
None present
Immunogen
C-terminal fusion protein of Rat NMDAR2B (30kDa) corresponding to amino acids 984-1104.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q00960
Entrez Gene
Grin2b
GO Terms
GO:0001508 regulation of action potential
GO:0001975 response to amphetamine
GO:0050839 cell adhesion molecule binding
GO:0004972 N-methyl-D-aspartate selective glutamate receptor activity
GO:0005234 extracellular-glutamate-gated ion channel activity
GO:0007616 long-term memory
GO:0008013 beta-catenin binding
GO:0008144 drug binding
GO:0008270 zinc ion binding
GO:0008306 associative learning
GO:0051707 response to other organism
GO:0009743 response to carbohydrate stimulus
GO:0010350 cellular response to magnesium starvation
GO:0010942 positive regulation of cell death
GO:0014049 positive regulation of glutamate secretion
GO:0014069 postsynaptic density
GO:0016600 flotillin complex
GO:0017146 N-methyl-D-aspartate selective glutamate receptor complex
GO:0021766 hippocampus development
GO:0021987 cerebral cortex development
GO:0030018 Z disc
GO:0030054 cell junction
GO:0030288 outer membrane-bounded periplasmic space
GO:0031749 D2 dopamine receptor binding
GO:0032026 response to magnesium ion
GO:0034097 response to cytokine stimulus
GO:0035235 ionotropic glutamate receptor signaling pathway
GO:0035255 ionotropic glutamate receptor binding
GO:0042165 neurotransmitter binding
GO:0042220 response to cocaine
GO:0042734 presynaptic membrane
GO:0043083 synaptic cleft
GO:0043113 receptor clustering
GO:0043195 terminal button
GO:0043197 dendritic spine
GO:0043408 regulation of MAPKKK cascade
GO:0045211 postsynaptic membrane
GO:0045471 response to ethanol
GO:0046982 protein heterodimerization activity
GO:0048169 regulation of long-term neuronal synaptic plasticity
GO:0048511 rhythmic process
GO:0050806 positive regulation of synaptic transmission
GO:0051592 response to calcium ion
GO:0051597 response to methylmercury
GO:0051602 response to electrical stimulus
GO:0060416 response to growth hormone stimulus
GO:0060992 response to fungicide
GO:0071230 cellular response to amino acid stimulus
GO:0071287 cellular response to manganese ion
GO:0071359 cellular response to dsRNA
GO:0071363 cellular response to growth factor stimulus
GO:0071396 cellular response to lipid
GO:0071407 cellular response to organic cyclic compound
GO:0071445 cellular response to protein stimulus
Regulatory
For research purposes only

Applications of Nmdar NR2B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/1000 1/2000
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
3 µl of antibody will quantitatively immunoprecipitate all NMDAR2B in 200 µg of rat brain. Optimal working dilutions must be determined by the end user.
Histology Positive Control Tissue
Hippocampus.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

References for Nmdar NR2B antibody

  1. Maliszewska-Cyna, E. et al. (2010) Diminished prevalence but preserved synaptic distribution of N-methyl-D-aspartate receptor subunits in the methyl CpG binding protein 2(MeCP2)-null mouse brain.
    Neuroscience. 168: 624-32
  2. Asaka, Y. et al. (2006) Hippocampal synaptic plasticity is impaired in the Mecp2-null mouse model of Rett syndrome.
    Neurobiol Dis. 21: 217-27.
  3. Grosshans, D.R. and Browning, M.D. (2001) Protein kinase C activation induces tyrosine phosphorylation of the NR2A and NR2B subunits of the NMDA receptor.
    J Neurochem. 76: 737-44.

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