Rabbit anti Rat NMDA Receptor NR2B antibody recognizes the ~180 kDa NMDA (N-methyl-D-aspartate) receptor NMDAR2B subunit.
NMDA receptors are a class of ionotropic glutamate receptors. NMDA channels have been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), or NMDAR2D (GRIN2D).
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 50µl of Phosphate buffered saline
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Ammonium bicarbonate
- Preservative Stabilisers
- None present
- C-terminal fusion protein of Rat NMDAR2B (30kDa) corresponding to amino acids 984-1104.
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of reconstitution.
- Entrez Gene
- GO Terms
regulation of action potential
response to amphetamine
cell adhesion molecule binding
N-methyl-D-aspartate selective glutamate receptor activity
extracellular-glutamate-gated ion channel activity
zinc ion binding
response to other organism
response to carbohydrate stimulus
cellular response to magnesium starvation
positive regulation of cell death
positive regulation of glutamate secretion
N-methyl-D-aspartate selective glutamate receptor complex
cerebral cortex development
outer membrane-bounded periplasmic space
D2 dopamine receptor binding
response to magnesium ion
response to cytokine stimulus
ionotropic glutamate receptor signaling pathway
ionotropic glutamate receptor binding
response to cocaine
regulation of MAPKKK cascade
response to ethanol
protein heterodimerization activity
regulation of long-term neuronal synaptic plasticity
positive regulation of synaptic transmission
response to calcium ion
response to methylmercury
response to electrical stimulus
response to growth hormone stimulus
response to fungicide
cellular response to amino acid stimulus
cellular response to manganese ion
cellular response to dsRNA
cellular response to growth factor stimulus
cellular response to lipid
cellular response to organic cyclic compound
cellular response to protein stimulus
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Nmdar NR2B antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- 3 µl of antibody will quantitatively immunoprecipitate all NMDAR2B in 200 µg of rat brain. Optimal working dilutions must be determined by the end user.
- Histology Positive Control Tissue
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for Nmdar NR2B antibody
Maliszewska-Cyna, E. et al. (2010) Diminished prevalence but preserved synaptic distribution of N-methyl-D-aspartate receptor subunits in the methyl CpG binding protein 2(MeCP2)-null mouse brain.
Neuroscience. 168: 624-32
Asaka, Y. et al. (2006) Hippocampal synaptic plasticity is impaired in the Mecp2-null mouse model of Rett syndrome.
Neurobiol Dis. 21: 217-27.
Grosshans, D.R. and Browning, M.D. (2001) Protein kinase C activation induces tyrosine phosphorylation of the NR2A and NR2B subunits of the NMDA receptor.
J Neurochem. 76: 737-44.