Nmdar NR2B antibody

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Rabbit anti Rat NMDA Receptor NR2B

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
6870-1109 Datasheet	C IP WB SDS	10 µg	Log in		Get Quote

Rabbit anti Rat NMDA Receptor NR2B antibody recognizes the ~180 kDa NMDA (N-methyl-D-aspartate) receptor NMDAR2B subunit.

NMDA receptors are a class of ionotropic glutamate receptors. NMDA channels have been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), or NMDAR2D (GRIN2D).

Product Details

Target Species

Rat

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Human
Fish

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - lyophilized

Reconstitution

Reconstitute with 50µl of Phosphate buffered saline

Preparation

Purified IgG prepared by affinity chromatography

Buffer Solution

Ammonium bicarbonate

Preservative Stabilisers

None present

Immunogen

C-terminal fusion protein of Rat NMDAR2B (30kDa) corresponding to amino acids 984-1104.

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: Q00960
Entrez Gene: Grin2b
GO Terms: GO:0001508 regulation of action potential; GO:0001975 response to amphetamine; GO:0050839 cell adhesion molecule binding; GO:0004972 N-methyl-D-aspartate selective glutamate receptor activity; GO:0005234 extracellular-glutamate-gated ion channel activity; GO:0007616 long-term memory; GO:0008013 beta-catenin binding; GO:0008144 drug binding; GO:0008270 zinc ion binding; GO:0008306 associative learning; GO:0051707 response to other organism; GO:0009743 response to carbohydrate stimulus; GO:0010350 cellular response to magnesium starvation; GO:0010942 positive regulation of cell death; GO:0014049 positive regulation of glutamate secretion; GO:0014069 postsynaptic density; GO:0016600 flotillin complex; GO:0017146 N-methyl-D-aspartate selective glutamate receptor complex; GO:0021766 hippocampus development; GO:0021987 cerebral cortex development; GO:0030018 Z disc; GO:0030054 cell junction; GO:0030288 outer membrane-bounded periplasmic space; GO:0031749 D2 dopamine receptor binding; GO:0032026 response to magnesium ion; GO:0034097 response to cytokine stimulus; GO:0035235 ionotropic glutamate receptor signaling pathway; GO:0035255 ionotropic glutamate receptor binding; GO:0042165 neurotransmitter binding; GO:0042220 response to cocaine; GO:0042734 presynaptic membrane; GO:0043083 synaptic cleft; GO:0043113 receptor clustering; GO:0043195 terminal button; GO:0043197 dendritic spine; GO:0043408 regulation of MAPKKK cascade; GO:0045211 postsynaptic membrane; GO:0045471 response to ethanol; GO:0046982 protein heterodimerization activity; GO:0048169 regulation of long-term neuronal synaptic plasticity; GO:0048511 rhythmic process; GO:0050806 positive regulation of synaptic transmission; GO:0051592 response to calcium ion; GO:0051597 response to methylmercury; GO:0051602 response to electrical stimulus; GO:0060416 response to growth hormone stimulus; GO:0060992 response to fungicide; GO:0071230 cellular response to amino acid stimulus; GO:0071287 cellular response to manganese ion; GO:0071359 cellular response to dsRNA; GO:0071363 cellular response to growth factor stimulus; GO:0071396 cellular response to lipid; GO:0071407 cellular response to organic cyclic compound; GO:0071445 cellular response to protein stimulus
Regulatory: For research purposes only

Applications of Nmdar NR2B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunohistology - Frozen	1/1000	1/2000
Immunoprecipitation
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: 3 µl of antibody will quantitatively immunoprecipitate all NMDAR2B in 200 µg of rat brain. Optimal working dilutions must be determined by the end user.
Histology Positive Control Tissue: Hippocampus.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:FITC	STAR34B	C F	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml	Log in	Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in		Get Quote

Product Specific References

References for Nmdar NR2B antibody

Maliszewska-Cyna, E. et al. (2010) Diminished prevalence but preserved synaptic distribution of N-methyl-D-aspartate receptor subunits in the methyl CpG binding protein 2(MeCP2)-null mouse brain.
Neuroscience. 168: 624-32
Asaka, Y. et al. (2006) Hippocampal synaptic plasticity is impaired in the Mecp2-null mouse model of Rett syndrome.
Neurobiol Dis. 21: 217-27.
Grosshans, D.R. and Browning, M.D. (2001) Protein kinase C activation induces tyrosine phosphorylation of the NR2A and NR2B subunits of the NMDA receptor.
J Neurochem. 76: 737-44.

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