MUNC-18 (pSer515) antibody

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Rabbit anti MUNC-18 (pSer515)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1234 WB* 0.1 ml
Rabbit anti Rat MUNC-18 (pSer515) antibody recognizes MUNC-18, also known as syntaxin-binding protein 1, N-Sec1, protein unc-18 homolog 1 (Unc18-1) and p67, when phosphorylated at serine 515. MUNC-18 is a ~67 kDa member of the STXBP/UNC-18/SEC1 family. It is neural specific, expressed in the brain, spinal cord and at very high levels in axons. Munc-18 is crucial to calcium-dependent neurotransmitter release, facilitating synaptic vesicle docking and fusion. It inhibits the formation of soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) complex by binding syntaxin 1 preventing the other components (synaptobrevin and synaptosomal associated protein of 25 kDa (SNAP-25)) from binding. The function of Munc-18 is regulated by the phosphorylation of serine 515 by protein kinase C.

Munc-18 is also involved in the onset of female puberty through its regulation of glutamate release (Kim et al. 2006).

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
Xenopus Expected from Sequence
Human Expected from Sequence
Bovine Expected from Sequence
Chicken Expected from Sequence
Mouse Expected from Sequence
Primate Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to rat Munc-18 (pSer515) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM Hepes pH7.5
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
50% Glycerol
0.01%Bovine Serum Albumin
Immunogen
Phosphopeptide corresponding to amino acid sequence surrounding phosphorylated Ser515 of Munc-18.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P61765 Related reagents
Entrez Gene
Stxbp1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005829 cytosol
GO:0006904 vesicle docking involved in exocytosis
GO:0042802 identical protein binding
GO:0010807 regulation of synaptic vesicle priming
GO:0015031 protein transport
GO:0050821 protein stabilization
GO:0017075 syntaxin-1 binding
GO:0019901 protein kinase binding
GO:0019904 protein domain specific binding
GO:0043234 protein complex
GO:0047485 protein N-terminus binding
Regulatory
For research purposes only

Applications of MUNC-18 (pSer515) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 1/1000
  1. 1For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1234 detects a band/s of approximately 65kDa in rat cortex cell lysates

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

References for MUNC-18 (pSer515) antibody

  1. Pevsner, J. et al. (1994) n-Sec1: a neural-specific syntaxin-binding protein.
    Proc Natl Acad Sci U S A. 91 (4): 1445-9.
  2. Yerrapureddy, A. et al. (2005) Intracellular interaction between syntaxin and Munc 18-1 revealed by fluorescence resonance energy transfer.
    Mol Membr Biol. 22 (5): 401-10.

Further Reading

  1. Toonen, R.F. et al. (2006) Munc18-1 expression levels control synapse recovery by regulating readily releasable pool size.
    Proc Natl Acad Sci U S A. 103 (48): 18332-7.