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MUNC-18 (pSer515) antibody

Rabbit anti MUNC-18 (pSer515)

Product Type
Polyclonal Antibody
Polyclonal IgG
MUNC-18 (pSer515)

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 ml loader
List Price Your Price

Rabbit anti Rat MUNC-18 (pSer515) antibody recognizes MUNC-18, also known as syntaxin-binding protein 1, N-Sec1, protein unc-18 homolog 1 (Unc18-1) and p67, when phosphorylated at serine 515. MUNC-18 is a ~67 kDa member of the STXBP/UNC-18/SEC1 family. It is neural specific, expressed in the brain, spinal cord and at very high levels in axons. Munc-18 is crucial to calcium-dependent neurotransmitter release, facilitating synaptic vesicle docking and fusion. It inhibits the formation of soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) complex by binding syntaxin 1 preventing the other components (synaptobrevin and synaptosomal associated protein of 25 kDa (SNAP-25)) from binding. The function of Munc-18 is regulated by the phosphorylation of serine 515 by protein kinase C.

Munc-18 is also involved in the onset of female puberty through its regulation of glutamate release (Kim et al. 2006).

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
Xenopus Expected from Sequence
Human Expected from Sequence
Bovine Expected from Sequence
Chicken Expected from Sequence
Mouse Expected from Sequence
Primate Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to rat Munc-18 (pSer515) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM Hepes pH7.5
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
50% Glycerol
0.01%Bovine Serum Albumin
Phosphopeptide corresponding to amino acid sequence surrounding phosphorylated Ser515 of Munc-18.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1234 detects a band/s of approximately 65kDa in rat cortex cell lysates

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Further Reading

  1. Pevsner, J. et al. (1994) n-Sec1: a neural-specific syntaxin-binding protein.
    Proc Natl Acad Sci U S A. 91 (4): 1445-9.
  2. Yerrapureddy, A. et al. (2005) Intracellular interaction between syntaxin and Munc 18-1 revealed by fluorescence resonance energy transfer.
    Mol Membr Biol. 22 (5): 401-10.
  3. Toonen, R.F. et al. (2006) Munc18-1 expression levels control synapse recovery by regulating readily releasable pool size.
    Proc Natl Acad Sci U S A. 103 (48): 18332-7.

Entrez Gene
GO Terms
GO:0005886 plasma membrane
GO:0005829 cytosol
GO:0006904 vesicle docking involved in exocytosis
GO:0042802 identical protein binding
GO:0010807 regulation of synaptic vesicle priming
GO:0015031 protein transport
GO:0050821 protein stabilization
GO:0017075 syntaxin-1 binding
GO:0019901 protein kinase binding
GO:0019904 protein domain specific binding
GO:0043234 protein complex
GO:0047485 protein N-terminus binding



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