MUNC-18 (pSer515) antibody
Rabbit anti MUNC-18 (pSer515)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- MUNC-18 (pSer515)
Rabbit anti Rat MUNC-18 (pSer515) antibody recognizes MUNC-18, also known as syntaxin-binding protein 1, N-Sec1, protein unc-18 homolog 1 (Unc18-1) and p67, when phosphorylated at serine 515. MUNC-18 is a ~67 kDa member of the STXBP/UNC-18/SEC1 family. It is neural specific, expressed in the brain, spinal cord and at very high levels in axons. Munc-18 is crucial to calcium-dependent neurotransmitter release, facilitating synaptic vesicle docking and fusion. It inhibits the formation of soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) complex by binding syntaxin 1 preventing the other components (synaptobrevin and synaptosomal associated protein of 25 kDa (SNAP-25)) from binding. The function of Munc-18 is regulated by the phosphorylation of serine 515 by protein kinase C. Munc-18 is also involved in the onset of female puberty through its regulation of glutamate release (Kim et al. 2006). |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Expected from Sequence Xenopus Expected from Sequence Human Expected from Sequence Bovine Expected from Sequence Chicken Expected from Sequence Mouse Expected from Sequence Primate Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to rat Munc-18 (pSer515) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- 10mM Hepes pH7.5
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 50% Glycerol 0.01% Bovine Serum Albumin - Immunogen
- Phosphopeptide corresponding to amino acid sequence surrounding phosphorylated Ser515 of Munc-18.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 1/1000 |
- Western Blotting
- AHP1234 detects a band/s of approximately 65kDa in rat cortex cell lysates
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Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg | Log in | |||
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Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg | Log in | |||
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Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg | Log in | |||
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Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg | Log in | |||
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Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml | Log in | |||
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Description | Sheep anti Rabbit IgG:RPE |
Further Reading
-
Pevsner, J. et al. (1994) n-Sec1: a neural-specific syntaxin-binding protein.
Proc Natl Acad Sci U S A. 91 (4): 1445-9. -
Yerrapureddy, A. et al. (2005) Intracellular interaction between syntaxin and Munc 18-1 revealed by fluorescence resonance energy transfer.
Mol Membr Biol. 22 (5): 401-10. -
Toonen, R.F. et al. (2006) Munc18-1 expression levels control synapse recovery by regulating readily releasable pool size.
Proc Natl Acad Sci U S A. 103 (48): 18332-7.
- RRID
- AB_877490
- UniProt
- P61765
- Entrez Gene
- Stxbp1
- GO Terms
- GO:0005886 plasma membrane
- GO:0005829 cytosol
- GO:0006904 vesicle docking involved in exocytosis
- GO:0042802 identical protein binding
- GO:0010807 regulation of synaptic vesicle priming
- GO:0015031 protein transport
- GO:0050821 protein stabilization
- GO:0017075 syntaxin-1 binding
- GO:0019901 protein kinase binding
- View More GO Terms
- GO:0019904 protein domain specific binding
- GO:0043234 protein complex
- GO:0047485 protein N-terminus binding
AHP1234
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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