Rabbit anti Rat Interleukin-4 antibody specifically recognizes rat interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, a pleiotropic cytokine which interacts with multiple cell lineages, including T cells, fibroblasts and haematopoietic cells, and is one of the most important factors involved in B cell IgG1 and IgE class switching. IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes.
- Target Species
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 0.1ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended
- Antiserum Preparation
- Antisera to rat IL-4 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present.
- Carrier Free
- Recombinant rat IL-4.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
negative regulation of acute inflammatory response
negative regulation of chronic inflammatory response
interleukin-4 receptor binding
response to nutrient
growth factor activity
response to drug
regulation of proton transport
response to organic cyclic compound
positive regulation of interleukin-10 production
response to cytokine stimulus
B cell activation
positive regulation of gene-specific transcription
negative regulation of nitric oxide biosynthetic process
response to ethanol
cellular response to mercury ion
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IL-4 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available