IL-4 antibody

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Rabbit anti Rat Interleukin-4

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAR16G E WB 0.1 mg
Rabbit anti Rat Interleukin-4 antibody specifically recognizes rat interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, a pleiotropic cytokine which interacts with multiple cell lineages, including T cells, fibroblasts and haematopoietic cells, and is one of the most important factors involved in B cell IgG1 and IgE class switching. IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes.

Product Details

Target Species
Rat
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended
Antiserum Preparation
Antisera to rat IL-4 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present.
Immunogen
Recombinant rat IL-4.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution.

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P20096 Related reagents
Entrez Gene
Il4 Related reagents
GO Terms
GO:0002674 negative regulation of acute inflammatory response
GO:0002677 negative regulation of chronic inflammatory response
GO:0005125 cytokine activity
GO:0005136 interleukin-4 receptor binding
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007565 female pregnancy
GO:0007584 response to nutrient
GO:0008083 growth factor activity
GO:0042493 response to drug
GO:0010155 regulation of proton transport
GO:0014070 response to organic cyclic compound
GO:0032733 positive regulation of interleukin-10 production
GO:0034097 response to cytokine stimulus
GO:0042113 B cell activation
GO:0043193 positive regulation of gene-specific transcription
GO:0045019 negative regulation of nitric oxide biosynthetic process
GO:0045471 response to ethanol
GO:0071288 cellular response to mercury ion
Regulatory
For research purposes only

Applications of IL-4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*