IgM antibody

Goat anti Rat IgM:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgM

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302002
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Goat anti Rat IgM antibody recognizes rat IgM as demonstrated by ELISA. This antibody has been cross-absorbed against rat IgG, but may react with IgM from other species.

Target Species
Rat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to rat IgM were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on rat IgM covalently linked to agarose.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Normal rat IgM.
Approx. Protein Concentrations
1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for IgM antibody

  1. Brissette, C.A. et al. (2012) Acetate supplementation reduces microglia activation and brain interleukin-1β levels in a rat model of Lyme neuroborreliosis.
    J Neuroinflammation. 9: 249.

ELISA

RRID
AB_616791
View more products with IGM specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rat Products