IgG2a antibody

Goat anti Rat IgG2a:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG2a

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STAR113F
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Goat anti Rat IgG2a antibody recognizes rat IgG2a as assessed by immunoelectrophoresis and ELISA.

Goat anti Rat IgG2a antibody may cross react with IgG2a from other species.

Target Species
Rat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to rat IgG2a were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 1 x 106 cells in 100ul.

References for IgG2a antibody

  1. Platt, R. et al. (2011) Comparison of humoral and cellular immune responses to inactivated swine influenza virus vaccine in weaned pigs.
    Vet Immunol Immunopathol. 142: 252-7.

RRID
AB_324440
UniProt
P20760
Entrez Gene
LOC679045
GO Terms
GO:0003823 antigen binding
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rat Products