IgG antibody

Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(MOUSE ADSORBED)

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New STAR71D550
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Goat anti Rat IgG (Mouse Adsorbed) antibody recognizes rat IgG. Cross-reactivity with mouse IgG has been minimised by adsorption.

Target Species
Rat
Product Form
Purified IgG conjugated to DyLight®550 - liquid
Antiserum Preparation
Antisera to rat IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Rat IgG.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Dylight®550 562 576
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/400
Immunofluorescence 1/50 1/400
Western Blotting 1/5000 1/25,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul.

References for IgG antibody

  1. Yang, X. et al. (2010) The role of the JAK2-STAT3 pathway in pro-inflammatory responses of EMF-stimulated N9 microglial cells.
    J Neuroinflammation. 7: 54.
  2. Tamayo, J. et al. (2001) Chemical sensors and biosensors in liquid environment based on microcantilevers with amplified quality factor.
    Ultramicroscopy. 86: 167-73.
  3. Pérez-Bosque A et al. (2004) Dietary plasma protein affects the immune response of weaned rats challenged with S. aureus Superantigen B.
    J Nutr. 134: 2667-72.
  4. Balan, P. et al. (2010) Immunomodulatory effects of ovine serum immunoglobulin in the growing rat.
    Animal. 4: 1702-8.
  5. Tulinská, J. et al. (2018) Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE).
    Arch Toxicol. 92 (7): 2385-99.

UniProt
P20759
P20762
P20761
P20760
Entrez Gene
Ighg
LOC679045
LOC362795
GO Terms
GO:0003823 antigen binding
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rat Products