IgG antibody

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WB ResetRabbit F(ab')2 anti Rat IgG:HRP
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
Horseradish peroxidase conjugated Rabbit F(ab')2 anti Rat IgG antibody recognizes Rat immunoglobulin G. Sera from rabbits hyperimmunised with highly purified rat IgG was affinity purified on a rat IgG column. Digestion to F(ab')2 with pepsin was then performed, the resulting fragments were conjugated to horseradish peroxidase using the periodate method. Cross reactivity is observed with mouse IgG but may be blocked by the addition of 10% mouse serum. |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography of serum
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Purified rat immunoglobulin G.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
1/500 | 1/1000 |
Immunohistology - Frozen | ![]() |
1/50 | |
Immunohistology - Paraffin | ![]() |
1/50 | |
Immunohistology - Resin | ![]() |
1/50 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | Log in | |||
List Price | Your Price | ||||||
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Description | AbGUARD® HRP Stabilizer Plus | ||||||
AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | Log in | |||
List Price | Your Price | ||||||
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Description | AbGUARD® HRP Stabilizer Plus | ||||||
AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | Log in | |||
List Price | Your Price | ||||||
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Description | AbGUARD® HRP Stabilizer Plus | ||||||
TMB Signal+ | BUF054A | E | 100 ml | Log in | |||
List Price | Your Price | ||||||
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Description | TMB Signal+ | ||||||
TMB Core | BUF056A | E | 100 ml | Log in | |||
List Price | Your Price | ||||||
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Description | TMB Core | ||||||
TMB Core+ | BUF062A | E | 100 ml | Log in | |||
List Price | Your Price | ||||||
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Description | TMB Core+ |
References for IgG antibody
-
Hampson, G. et al. (2010) Validation of an ELISA for the determination of rituximab pharmacokinetics in clinical trials subjects.
J Immunol Methods. 360 (1-2): 30-8. -
Modjtahedi, H. et al. (2012) Immunohistochemical discrimination of wild-type EGFR from EGFRvIII in fixed tumour specimens using anti-EGFR mAbs ICR9 and ICR10.
Br J Cancer. 106 (5): 883-8. -
Gholami, M. et al. (2015) Selenium effect on ischemia-reperfusion injury of gastrocnemius muscle in adult rats.
Biol Trace Elem Res. 164 (2): 205-11. -
Zschemisch NH et al. (2016) Transcription Factor SP2 Enhanced the Expression of Cd14 in Colitis-Susceptible C3H/HeJBir.
PLoS One. 11 (5): e0155821. -
Gholami, M.R. et al. (2020) Protective effects of honey, Apis mellifera meda. skorikov, on ischemia-reperfusion induced muscle injury.
Int J Morphol., 38 (3): 804-10,
STAR21B



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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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