IgG antibody
F(ab')2 Rabbit anti Rat IgG antibody used for the detection of bound rat antibodies to Mouse CD14 and beta actin in western blotting
Image caption:
Transfection system establishment.
A Western blot analysis of CD14 expression in untreated and LPS stimulated murine RAW264.7 macrophages and NIH/3T3 fibroblasts, β-actin as endogeous control. Transfection efficiency test by determination of GFP-positive RAW264.7 cells by cyto fluorescence 24 hours after transfection with B Xtreme Gene HP, C Xtreme Gene 9 DNA, D Superfect, E Polyfect, and F by β-galactosidase assay, ***: p < 0,05.
From: Zschemisch N-H, Brüsch I, Hambusch A-S, Bleich A (2016)
Transcription Factor SP2 Enhanced the Expression of Cd14 in Colitis-Susceptible C3H/HeJBir.
PLoS ONE 11(5): e0155821.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB ResetRabbit F(ab')2 anti Rat IgG:HRP
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
Antibody Quality and Performance Guarantee
| Horseradish peroxidase conjugated Rabbit F(ab')2 anti Rat IgG antibody recognizes Rat immunoglobulin G. Sera from rabbits hyperimmunised with highly purified rat IgG was affinity purified on a rat IgG column. Digestion to F(ab')2 with pepsin was then performed, the resulting fragments were conjugated to horseradish peroxidase using the periodate method. Cross reactivity is observed with mouse IgG but may be blocked by the addition of 10% mouse serum. |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography of serum
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Purified rat immunoglobulin G.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/500 | 1/1000 |
| Immunohistology - Frozen | |
1/50 | |
| Immunohistology - Paraffin | |
1/50 | |
| Immunohistology - Resin | |
1/50 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| TMB Signal+ | BUF054A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TMB Signal+ | ||||||
| TMB Core | BUF056A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TMB Core | ||||||
| TMB Core+ | BUF062A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TMB Core+ | ||||||
References for IgG antibody
-
Hampson, G. et al. (2010) Validation of an ELISA for the determination of rituximab pharmacokinetics in clinical trials subjects.
J Immunol Methods. 360 (1-2): 30-8. -
Modjtahedi, H. et al. (2012) Immunohistochemical discrimination of wild-type EGFR from EGFRvIII in fixed tumour specimens using anti-EGFR mAbs ICR9 and ICR10.
Br J Cancer. 106 (5): 883-8. -
Gholami, M. et al. (2015) Selenium effect on ischemia-reperfusion injury of gastrocnemius muscle in adult rats.
Biol Trace Elem Res. 164 (2): 205-11. -
Zschemisch NH et al. (2016) Transcription Factor SP2 Enhanced the Expression of Cd14 in Colitis-Susceptible C3H/HeJBir.
PLoS One. 11 (5): e0155821. -
Gholami, M.R. et al. (2020) Protective effects of honey, Apis mellifera meda. skorikov, on ischemia-reperfusion induced muscle injury.
Int J Morphol., 38 (3): 804-10,
STAR21B
0615
159099
165235
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