Horseradish peroxidase conjugated Rabbit F(ab')2 anti Rat IgG antibody recognizes Rat immunoglobulin G. Sera from rabbits hyperimmunised with highly purified rat IgG was affinity purified on a rat IgG column. Digestion to F(ab')2 with pepsin was then performed, the resulting fragments were conjugated to horseradish peroxidase using the periodate method.
Cross reactivity is observed with mouse IgG but may be blocked by the addition of 10% mouse serum.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Purified IgG prepared by affinity chromatography of serum
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Purified rat immunoglobulin G.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IgG antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin
|Immunohistology - Resin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
Application Based External Images
Immunohistology - Paraffin
Product Specific References
References for IgG antibody
Hampson, G. et al. (2010) Validation of an ELISA for the determination of rituximab pharmacokinetics in clinical trials subjects.
J Immunol Methods. 360 (1-2): 30-8.
Modjtahedi, H. et al. (2012) Immunohistochemical discrimination of wild-type EGFR from EGFRvIII in fixed tumour specimens using anti-EGFR mAbs ICR9 and ICR10.
Br J Cancer. 106 (5): 883-8.
Gholami, M. et al. (2015) Selenium effect on ischemia-reperfusion injury of gastrocnemius muscle in adult rats.
Biol Trace Elem Res. 164 (2): 205-11.
Zschemisch NH et al. (2016) Transcription Factor SP2 Enhanced the Expression of Cd14 in Colitis-Susceptible C3H/HeJBir.
PLoS One. 11 (5): e0155821.
Gholami, M.R. et al. (2020) Protective effects of honey, Apis mellifera meda. skorikov, on ischemia-reperfusion induced muscle injury.
Int J Morphol., 38 (3): 804-10,
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