IgG antibody
F(ab')2 Rabbit anti Rat IgG antibody used for the detection of bound rat antibodies to Mouse CD14 and beta actin in western blotting
Image caption:
Transfection system establishment.
A Western blot analysis of CD14 expression in untreated and LPS stimulated murine RAW264.7 macrophages and NIH/3T3 fibroblasts, β-actin as endogeous control. Transfection efficiency test by determination of GFP-positive RAW264.7 cells by cyto fluorescence 24 hours after transfection with B Xtreme Gene HP, C Xtreme Gene 9 DNA, D Superfect, E Polyfect, and F by β-galactosidase assay, ***: p < 0,05.
From: Zschemisch N-H, Brüsch I, Hambusch A-S, Bleich A (2016)
Transcription Factor SP2 Enhanced the Expression of Cd14 in Colitis-Susceptible C3H/HeJBir.
PLoS ONE 11(5): e0155821.
Horseradish peroxidase conjugated Rabbit F(ab')2 anti Rat IgG antibody recognizes Rat immunoglobulin G. Sera from rabbits hyperimmunised with highly purified rat IgG was affinity purified on a rat IgG column. Digestion to F(ab')2 with pepsin was then performed, the resulting fragments were conjugated to horseradish peroxidase using the periodate method.
Cross reactivity is observed with mouse IgG but may be blocked by the addition of 10% mouse serum.
Cross reactivity is observed with mouse IgG but may be blocked by the addition of 10% mouse serum.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography of serum
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- HRP stabiliser (BUF052A)
0.01% Thiomersal - Immunogen
- Purified rat immunoglobulin G.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P20759 Related reagents
- P20761 Related reagents
- P20762 Related reagents
- P20760 Related reagents
- Entrez Gene
- Ighg Related reagents
- LOC679045 Related reagents
- LOC362795 Related reagents
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/500 | 1/1000 | |
| Immunohistology - Frozen | 1/50 | ||
| Immunohistology - Paraffin | 1/50 | ||
| Immunohistology - Resin | 1/50 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | 100 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | 500 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | 1000 ml | C E P WB | ||
| TMB Signal+ | BUF054A | 100 ml | E | ||
| TMB Core | BUF056A | 100 ml | E | ||
| TMB Core+ | BUF062A | 100 ml | E |
Product Specific References
References for IgG antibody
-
Hampson, G. et al. (2010) Validation of an ELISA for the determination of rituximab pharmacokinetics in clinical trials subjects.
J Immunol Methods. 360 (1-2): 30-8. -
Modjtahedi, H. et al. (2012) Immunohistochemical discrimination of wild-type EGFR from EGFRvIII in fixed tumour specimens using anti-EGFR mAbs ICR9 and ICR10.
Br J Cancer. 106 (5): 883-8. -
Gholami, M. et al. (2015) Selenium effect on ischemia-reperfusion injury of gastrocnemius muscle in adult rats.
Biol Trace Elem Res. 164 (2): 205-11. -
Zschemisch NH et al. (2016) Transcription Factor SP2 Enhanced the Expression of Cd14 in Colitis-Susceptible C3H/HeJBir.
PLoS One. 11 (5): e0155821.
