Rabbit F(ab')2 anti Rat IgG antibody recognizes Rat IgG.
- Target Species
- Product Form
- F(ab')2 fragment of IgG conjugated to Fluorescein Isothiocyanate Isomer I (FITC) - liquid
- Antiserum Preparation
- Antisera to rat IgG were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove the remaining intact IgG or Fc fragments.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IgG antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for IgG antibody
O-Charoenrat, P. et al. (2000) Epidermal growth factor-like ligands differentially up-regulate matrix metalloproteinase 9 in head and neck squamous carcinoma cells.
Cancer Res. 60: 1121 - 1128.
Leposavić, G. et al. (2010) Age-associated plasticity of α1-adrenoceptor-mediated tuning of T-cell development.
Exp Gerontol. 45 (12): 918-35.
Tiwari, S. (2011) Divalent cations regulate the folding and activation status of integrins during their intracellular trafficking.
J Cell Sci. 124: 1672-80.
Reynolds, J. et al. (2012) Stimulation of the PD-1/PDL-1 T-cell co-inhibitory pathway is effective in treatment of experimental autoimmune glomerulonephritis.
Nephrol Dial Transplant. 27 (4): 1343-50.
Villiers, E. et al. (2006) Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.
Vet Clin Pathol. 35 (1): 55-71.
Uner, A. G. et al. (2013) Blood Levels of Selected Metabolic Factors, Cytokines, and Lymphocyte Subpopulations in Arabian and Thoroughbred Horses During the Longest and Shortest Days of the Year
J of Equine Vet Sci. 33 (11): 969-976.
Willheim, M. et al. (1999) Regulatory effects of 1alpha,25-dihydroxyvitamin D3 on the cytokine production of human peripheral blood lymphocytes.
J Clin Endocrinol Metab. 84 (10): 3739-44.
Thien, R. et al. (2005) Interactions of 1 alpha,25-dihydroxyvitamin D3 with IL-12 and IL-4 on cytokine expression of human T lymphocytes.
J Allergy Clin Immunol. 116 (3): 683-9.
Cian, F. et al. (2014) Stability of immunophenotypic lymphoid markers in fixed canine peripheral blood for flow cytometric analysis.
Vet Clin Pathol. 43 (1): 101-8.
Ferreira-Dias G et al. (2005) Seasonal reproduction in the mare: possible role of plasma leptin, body weight and immune status.
Domest Anim Endocrinol. 29 (1): 203-13.
Liu, W. et al. (2015) Evaluation of Stem Cell Marker Expression in Canine B-Cell Lymphoma Cell Lines, B-Cell Lymphoma-generated Spheres and Primary Samples.
Anticancer Res. 35 (5): 2805-16.
Platt, R. et al. (2010) Cell-mediated immunity evaluation in foals infected with virulent equine herpesvirus-1 by multi-parameter flow cytometry.
Vet Immunol Immunopathol. 135 (3-4): 275-81.
Van de Velde, H. et al. (2012) Short-term increase of body weight triggers immunological variables in dogs.
Vet Immunol Immunopathol. 145 (1-2): 431-7.
Agrícola R et al. (2008) Blood lymphocyte subpopulations, neutrophil phagocytosis and proteinogram during late pregnancy and postpartum in mares.
Reprod Domest Anim. 43 (2): 212-7.
Liu, W. et al. (2020) Establishment and characterization of stable red, far-red (fR) and near infra-red (NIR) transfected canine prostate cancer cell lines.
Cancer Cell Int. 20: 139.
How to Use the Spectraviewer?
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra