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IgG antibody

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Rabbit F(ab')2 anti Rat IgG:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG

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Rabbit F(ab')2 anti Rat IgG antibody recognizes Rat IgG.

Target Species
Rat
Product Form
F(ab')2 fragment of IgG conjugated to Fluorescein Isothiocyanate Isomer I (FITC) - liquid
Antiserum Preparation
Antisera to rat IgG were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove the remaining intact IgG or Fc fragments.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/200 1/400
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

References for IgG antibody

  1. O-Charoenrat, P. et al. (2000) Epidermal growth factor-like ligands differentially up-regulate matrix metalloproteinase 9 in head and neck squamous carcinoma cells.
    Cancer Res. 60: 1121 - 1128.
  2. Leposavić, G. et al. (2010) Age-associated plasticity of α1-adrenoceptor-mediated tuning of T-cell development.
    Exp Gerontol. 45 (12): 918-35.
  3. Tiwari, S. (2011) Divalent cations regulate the folding and activation status of integrins during their intracellular trafficking.
    J Cell Sci. 124: 1672-80.
  4. Reynolds, J. et al. (2012) Stimulation of the PD-1/PDL-1 T-cell co-inhibitory pathway is effective in treatment of experimental autoimmune glomerulonephritis.
    Nephrol Dial Transplant. 27 (4): 1343-50.
  5. Villiers, E. et al. (2006) Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.
    Vet Clin Pathol. 35 (1): 55-71.
  6. Uner, A. G. et al. (2013) Blood Levels of Selected Metabolic Factors, Cytokines, and Lymphocyte Subpopulations in Arabian and Thoroughbred Horses During the Longest and Shortest Days of the Year
    J of Equine Vet Sci. 33 (11): 969-976.
  7. Willheim, M. et al. (1999) Regulatory effects of 1alpha,25-dihydroxyvitamin D3 on the cytokine production of human peripheral blood lymphocytes.
    J Clin Endocrinol Metab. 84 (10): 3739-44.
  8. Thien, R. et al. (2005) Interactions of 1 alpha,25-dihydroxyvitamin D3 with IL-12 and IL-4 on cytokine expression of human T lymphocytes.
    J Allergy Clin Immunol. 116 (3): 683-9.
    9. View The Latest Product References
  9. Cian, F. et al. (2014) Stability of immunophenotypic lymphoid markers in fixed canine peripheral blood for flow cytometric analysis.
    Vet Clin Pathol. 43 (1): 101-8.
  10. Ferreira-Dias G et al. (2005) Seasonal reproduction in the mare: possible role of plasma leptin, body weight and immune status.
    Domest Anim Endocrinol. 29 (1): 203-13.
  11. Liu, W. et al. (2015) Evaluation of Stem Cell Marker Expression in Canine B-Cell Lymphoma Cell Lines, B-Cell Lymphoma-generated Spheres and Primary Samples.
    Anticancer Res. 35 (5): 2805-16.
  12. Platt, R. et al. (2010) Cell-mediated immunity evaluation in foals infected with virulent equine herpesvirus-1 by multi-parameter flow cytometry.
    Vet Immunol Immunopathol. 135 (3-4): 275-81.
  13. Van de Velde, H. et al. (2012) Short-term increase of body weight triggers immunological variables in dogs.
    Vet Immunol Immunopathol. 145 (1-2): 431-7.
  14. Agrícola R et al. (2008) Blood lymphocyte subpopulations, neutrophil phagocytosis and proteinogram during late pregnancy and postpartum in mares.
    Reprod Domest Anim. 43 (2): 212-7.
  15. Liu, W. et al. (2020) Establishment and characterization of stable red, far-red (fR) and near infra-red (NIR) transfected canine prostate cancer cell lines.
    Cancer Cell Int. 20: 139.

RRID
AB_321939
UniProt
P20759
P20761
P20762
P20760
Entrez Gene
Ighg
LOC679045
LOC362795
GO Terms
GO:0003823 antigen binding
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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