IgG antibody

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Rabbit F(ab')2 anti Rat IgG:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
STAR17B F 1 mg
Rabbit F(ab')2 anti Rat IgG antibody recognizes Rat IgG.

Product Details

Target Species
Rat
Product Form
F(ab')2 fragment of IgG conjugated to Fluorescein Isothiocyanate Isomer I (FITC) - liquid
Antiserum Preparation
Antisera to rat IgG were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove the remaining intact IgG or Fc fragments.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P20759 Related reagents
P20761 Related reagents
P20762 Related reagents
P20760 Related reagents
Entrez Gene
Ighg Related reagents
LOC679045 Related reagents
LOC362795 Related reagents
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/200 1/400
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

Product Specific References

References for IgG antibody

  1. O-Charoenrat, P. et al. (2000) Epidermal growth factor-like ligands differentially up-regulate matrix metalloproteinase 9 in head and neck squamous carcinoma cells.
    Cancer Res. 60: 1121 - 1128.
  2. Leposavić, G. et al. (2010) Age-associated plasticity of α1-adrenoceptor-mediated tuning of T-cell development.
    Exp Gerontol. 45 (12): 918-35.
  3. Tiwari, S. (2011) Divalent cations regulate the folding and activation status of integrins during their intracellular trafficking.
    J Cell Sci. 124: 1672-80.
  4. Reynolds, J. et al. (2012) Stimulation of the PD-1/PDL-1 T-cell co-inhibitory pathway is effective in treatment of experimental autoimmune glomerulonephritis.
    Nephrol Dial Transplant. 27 (4): 1343-50.
  5. Villiers, E. et al. (2006) Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.
    Vet Clin Pathol. 35 (1): 55-71.
  6. Uner, A. G. et al. (2013) Blood Levels of Selected Metabolic Factors, Cytokines, and Lymphocyte Subpopulations in Arabian and Thoroughbred Horses During the Longest and Shortest Days of the Year
    J of Equine Vet Sci. 33 (11): 969-976.
  7. Willheim, M. et al. (1999) Regulatory effects of 1alpha,25-dihydroxyvitamin D3 on the cytokine production of human peripheral blood lymphocytes.
    J Clin Endocrinol Metab. 84 (10): 3739-44.
  8. Thien, R. et al. (2005) Interactions of 1 alpha,25-dihydroxyvitamin D3 with IL-12 and IL-4 on cytokine expression of human T lymphocytes.
    J Allergy Clin Immunol. 116 (3): 683-9.
  9. Cian, F. et al. (2014) Stability of immunophenotypic lymphoid markers in fixed canine peripheral blood for flow cytometric analysis.
    Vet Clin Pathol. 43 (1): 101-8.
  10. Ferreira-Dias G et al. (2005) Seasonal reproduction in the mare: possible role of plasma leptin, body weight and immune status.
    Domest Anim Endocrinol. 29 (1): 203-13.
  11. Liu, W. et al. (2015) Evaluation of Stem Cell Marker Expression in Canine B-Cell Lymphoma Cell Lines, B-Cell Lymphoma-generated Spheres and Primary Samples.
    Anticancer Res. 35 (5): 2805-16.
  12. Platt, R. et al. (2010) Cell-mediated immunity evaluation in foals infected with virulent equine herpesvirus-1 by multi-parameter flow cytometry.
    Vet Immunol Immunopathol. 135 (3-4): 275-81.
  13. Van de Velde, H. et al. (2012) Short-term increase of body weight triggers immunological variables in dogs.
    Vet Immunol Immunopathol. 145 (1-2): 431-7.
  14. Agrícola R et al. (2008) Blood lymphocyte subpopulations, neutrophil phagocytosis and proteinogram during late pregnancy and postpartum in mares.
    Reprod Domest Anim. 43 (2): 212-7.