IgG antibody

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Anti Rat IgG Antibody gallery image 1 — Western blot analysis of Jurkat whole cell lysate (20 µg). Lane A probed with Rat anti tubulin alpha antibody (VMA00051) at a 1/1000 dilution followed by detection with Dylight550 conjugated Rabbit F(ab’)2 anti Rat IgG (**STAR16D550**) at a 1/10,000 dilution. Lane B probed with Rabbit F(ab’)2 anti Rat IgG (**STAR16D550**) at a 1/10,000 dilution. Performed under reduced conditions. Visualized on ChemiDoc Touch imaging system. Arrow points to tubulin alpha (molecular weight 52kDa).

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Rabbit F(ab')2 anti Rat IgG:DyLight®550

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: IgG

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STAR16D550 Datasheet Datasheet SDS SDS	F IF WB	0.1 mg		Log in	Get Quote
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Rabbit F(ab')2 anti Rat IgG antibody recognizes rat IgG, detecting all subclasses in immunodiffusion assays.

Target Species: Rat
Product Form: Purified IgG conjugated to DyLight^®550 - liquid
Antiserum Preparation: Antisera to rat IgG were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Purified polyclonal rat IgG.
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

Dylight®550 562 576
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: DyLight^® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Fluorophore	Excitation Max (nm)	Emission Max (nm)
Dylight®550	562	576

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	1/100	1/400
Immunofluorescence	1/100	1/400
Western Blotting	1/10,000	1/50,000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.