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IgG antibody

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Anti Rat IgG (Mouse Adsorbed) Ab - AP thumbnail image 1
Anti Rat IgG (Mouse Adsorbed) Ab - AP gallery image 1
Published customer image:
Biotinylated Goat anti Rat IgG antibody (STAR131B) used to detect bound Rat anti Murine F4/80 antibody, clone A3-1 (MCA497GA) on formalin fixed, paraffin embedded tissue sections by immunohistochemistry.
Image caption:
Tubulointerstitial inflammation and loss of peritubular capillaries in CKD kidneys.
Kidney sections of control and CKD mice were stained for macrophages (A), neutrophils (B), and vascular endothelium (C). Shown are representative immunohistochemical stainings of control and CKD kidneys and quantitative analyses of each staining categorized for renal cortex and outer medulla. Black bars represent control (n = 15) and dashed bars CKD kidneys (n = 16). Scale bars, 50μm. ** p<0.01.

From: Schley G, Jordan J, Ellmann S, Rosen S, Eckardt K-U, Uder M, et al. (2018)
Multiparametric magnetic resonance imaging of experimental chronic kidney disease: A quantitative correlation study with histology.
PLoS ONE 13(7): e0200259.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG

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STAR131A
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Goat anti Rat IgG antibody recognizes rat IgG. This antibody has been cross absorbed against pooled mouse sera and pooled mouse plasmacytoma/hybridoma proteins.

Target Species
Rat
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Antiserum Preparation
Antisera to rat IgG were raised by repeated immunisation of goats with purified antigen. Purified IgG prepared by affinity chromatography
Buffer Solution
TRIS buffered saline, 1mM MgCl2
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
50% Glycerol
Immunogen
Rat IgG
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/4000
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

References for IgG antibody

  1. Monticone, S. et al. (2015) Immunohistochemical, genetic and clinical characterization of sporadic aldosterone-producing adenomas.
    Mol Cell Endocrinol. pii: S0303-7207(15)00223-3.
  2. Schley, G. et al. (2018) Multiparametric magnetic resonance imaging of experimental chronic kidney disease: A quantitative correlation study with histology.
    PLoS One. 13 (7): e0200259.
  3. El Amki, M. et al. (2018) Improved Reperfusion and Vasculoprotection by the Poly(ADP-Ribose)Polymerase Inhibitor PJ34 After Stroke and Thrombolysis in Mice.
    Mol Neurobiol. 55 (12): 9156-9168.

RRID
AB_1102642
UniProt
P20761
P20762
P20760
P20759
Entrez Gene
Ighg
LOC679045
LOC362795
GO Terms
GO:0003823 antigen binding
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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