IgG antibody

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Anti Rat IgG (Mouse Adsorbed) Ab - AP gallery image 1 — **Published customer image:**
Biotinylated Goat anti Rat IgG antibody (**STAR131B**) used to detect bound Rat anti Murine F4/80 antibody, clone A3-1 (MCA497GA) on formalin fixed, paraffin embedded tissue sections by immunohistochemistry.
**Image caption:**
Tubulointerstitial inflammation and loss of peritubular capillaries in CKD kidneys.
Kidney sections of control and CKD mice were stained for macrophages (A), neutrophils (B), and vascular endothelium (C). Shown are representative immunohistochemical stainings of control and CKD kidneys and quantitative analyses of each staining categorized for renal cortex and outer medulla. Black bars represent control (n = 15) and dashed bars CKD kidneys (n = 16). Scale bars, 50μm. ** p<0.01.

From: Schley G, Jordan J, Ellmann S, Rosen S, Eckardt K-U, Uder M, *et al*. (2018)
Multiparametric magnetic resonance imaging of experimental chronic kidney disease: A quantitative correlation study with histology.
PLoS ONE 13(7): e0200259.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)

Goat anti Rat IgG:Biotin (Mouse Adsorbed)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
STAR131A Datasheet	C E P WB SDS	1 ml	Log in		Get Quote
STAR131B Datasheet	C E IF P WB SDS	0.5 mg	Log in		Get Quote

Goat anti Rat IgG antibody recognizes rat IgG. This antibody has been cross absorbed against pooled mouse sera and pooled mouse plasmacytoma/hybridoma proteins.

Product Details

Target Species: Rat
Product Form: Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form: Purified IgG conjugated to Biotin - liquid
Antiserum Preparation: Antisera to rat IgG were raised by repeated immunisation of goats with purified antigen. Purified IgG prepared by affinity chromatography
Buffer Solution: TRIS buffered saline, 1mM MgCl2
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
50% Glycerol
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
Immunogen: Rat IgG
Approx. Protein Concentrations: IgG concentration 0.5mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: Guaranteed until date of expiry. Please see product label.
Guarantee: 12 months from date of despatch

More Information

UniProt: P20761; P20762; P20760; P20759
Entrez Gene: Ighg; LOC679045; LOC362795
GO Terms: GO:0003823 antigen binding
Regulatory: For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/2000	1/4000
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
ELISA	1/5000	1/20000
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Product Specific References

References for IgG antibody

Monticone, S. et al. (2015) Immunohistochemical, genetic and clinical characterization of sporadic aldosterone-producing adenomas.
Mol Cell Endocrinol. pii: S0303-7207(15)00223-3.
Schley, G. et al. (2018) Multiparametric magnetic resonance imaging of experimental chronic kidney disease: A quantitative correlation study with histology.
PLoS One. 13 (7): e0200259.
El Amki, M. et al. (2018) Improved Reperfusion and Vasculoprotection by the Poly(ADP-Ribose)Polymerase Inhibitor PJ34 After Stroke and Thrombolysis in Mice.
Mol Neurobiol. 55 (12): 9156-9168.

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