IgG antibody
Biotinylated Goat anti Rat IgG antibody (STAR131B) used to detect bound Rat anti Murine F4/80 antibody, clone A3-1 (MCA497GA) on formalin fixed, paraffin embedded tissue sections by immunohistochemistry.
Image caption:
Tubulointerstitial inflammation and loss of peritubular capillaries in CKD kidneys.
Kidney sections of control and CKD mice were stained for macrophages (A), neutrophils (B), and vascular endothelium (C). Shown are representative immunohistochemical stainings of control and CKD kidneys and quantitative analyses of each staining categorized for renal cortex and outer medulla. Black bars represent control (n = 15) and dashed bars CKD kidneys (n = 16). Scale bars, 50μm. ** p<0.01.
From: Schley G, Jordan J, Ellmann S, Rosen S, Eckardt K-U, Uder M, et al. (2018)
Multiparametric magnetic resonance imaging of experimental chronic kidney disease: A quantitative correlation study with histology.
PLoS ONE 13(7): e0200259.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
P ResetGoat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
Antibody Quality and Performance Guarantee
| Goat anti Rat IgG antibody recognizes rat IgG. This antibody has been cross absorbed against pooled mouse sera and pooled mouse plasmacytoma/hybridoma proteins. |
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Antiserum Preparation
- Antisera to rat IgG were raised by repeated immunisation of goats with purified antigen. Purified IgG prepared by affinity chromatography
- Buffer Solution
- TRIS buffered saline, 1mM MgCl2
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
50% Glycerol - Immunogen
- Rat IgG
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/2000 | 1/4000 |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
||
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for IgG antibody
-
Monticone, S. et al. (2015) Immunohistochemical, genetic and clinical characterization of sporadic aldosterone-producing adenomas.
Mol Cell Endocrinol. pii: S0303-7207(15)00223-3. -
Schley, G. et al. (2018) Multiparametric magnetic resonance imaging of experimental chronic kidney disease: A quantitative correlation study with histology.
PLoS One. 13 (7): e0200259. -
El Amki, M. et al. (2018) Improved Reperfusion and Vasculoprotection by the Poly(ADP-Ribose)Polymerase Inhibitor PJ34 After Stroke and Thrombolysis in Mice.
Mol Neurobiol. 55 (12): 9156-9168. -
Gawish, R. et al. (2022) A neutrophil-B-cell axis impacts tissue damage control in a mouse model of intraabdominal bacterial infection via Cxcr4.
Elife. 11:e78291.
- RRID
- AB_1102642
- UniProt
- P20761
- P20762
- P20760
- P20759
- Entrez Gene
- Ighg
- LOC679045
- LOC362795
- GO Terms
- GO:0003823 antigen binding
STAR131A
155680
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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