IgG antibody

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Rabbit F(ab')2 anti Rat IgG:HRP

Product Type
Polyclonal Antibody
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
1 ml loader

Rabbit F(ab′)2 anti Rat IgG antibody recognizes the heavy and light chains of rat IgG1, IgG2a, IgG2b and IgG2c. This product also reacts with the light chains of rat IgM as demonstrated by ELISA.

Product Details

Target Species
Product Form
F(ab')2 fragment of IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to rat IgG was raised by repeated immunisations of rabbits with rat IgG paraproteins. The preparation was purified by affinity chromatography on rat IgG covalently linked to agarose, and gel filtration chromatography of pepsin digested antibody.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
50% Glycerol
Rat IgG.

Storage Information

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend
Guaranteed until date of expiry. Please see product label.

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GO Terms
GO:0003823 antigen binding
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/4000 1/8000
Immunoblotting 1/1000 1/2000
Immunohistology - Frozen 1/50 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml