IgA antibody

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Goat anti Rat IgA

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: IgA

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Secondary Antibodies
References (2)
Applications
Storage Information
Batch/Lot Specific Datasheets

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STAR111 Datasheet SDS SDS	E IF WB	0.5 mg		Log in
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Anti Rat IgA Antibody gallery image 1 — **Published customer image:**
Horseradish peroxidase conjugated Goat anti Rat IgA antibody (**STAR111P**) used to evaluate post challenge antibody response of rats by ELISA.
**Image caption:**
Post-challenge serum antibody isotype responses of vaccinated and control rats to recombinant cysteine proteinase.
(A) IgG1. (B) IgM. (C) IgA. (D) IgE. Group G–Lyophilised lettuce expressing the mature CPFhW enzyme flanked by Gly-rich linkers; Group U–Lyophilised lettuce expressing the mature CPFhW protein fused with ubiquitin; Group C–Lyophilised control lettuce; Group N–None. At each timepoint, four rats from each group were euthanised and dissected. *Indicates significantly increased numbers of cells (p<0.05). Error bars indicate standard deviation.

From: Kesik-Brodacka M, Lipiec A, Kozak Ljunggren M, Jedlina L, Miedzinska K, Mikolajczak M, et al. (2017)
Immune response of rats vaccinated orally with various plant-expressed recombinant cysteine proteinase constructs when challenged with Fasciola hepatica metacercariae.
PLoS Negl Trop Dis 11(3): e0005451.

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Goat anti Rat IgA antibody recognizes rat IgA as assessed by immunoelectrophoresis and ELISA.

Goat anti Rat IgA antibody may cross react with IgA from other species.

Target Species: Rat
Product Form: Purified IgG - liquid
Antiserum Preparation: Antisera to rat IgA were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature.
Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1ug/ml	10ug/ml
Immunofluorescence
Western Blotting

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Description	Rabbit anti Goat IgG (Fc):HRP

References for IgA antibody

Liu, Y. et al. (2008) The immunohistochemical localization of secretory IgA in the submandibular gland of the Mongolian gerbil
ACMS 4: 22-5
Kesik-brodacka, M. et al. (2017) Immune response of rats vaccinated orally with various plant-expressed recombinant cysteine proteinase constructs when challenged with Fasciola hepatica metacercariae.
PLoS Negl Trop Dis. 11 (3): e0005451.