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GSK3 Beta (pSer9) antibody

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Anti GSK3 Beta (pSer9) Antibody gallery image 1
Western blot of rat cortex lysate showing phosphospecific immunolabeling of the ~46 kDa GSK3β protein phosphorylated at Ser9 using Rabbit anti Rat GSK3 beta (pSer9) antibody (AHP1233).

Rabbit anti GSK3 Beta (pSer9)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1233 WB* 0.1 ml
Rabbit anti Rat GSK3 beta (pSer9) antibody recognizes GSK3 beta, also known as glycogen synthase kinase-3 beta (GSK-3 beta), factor A (FA) and serine/threonine-protein kinase GSK3B, when phosphorylated at serine 9. GSK3 is a ~46 kDa member of the serine/threonine protein kinase family. Two isforms of GSK3 exist, alpha and beta. GSK3 beta is expressed in the testis, thymus, prostate, and ovary as well as being expressed at low levels in lung, brain and kidney tissue.

GSK3 beta is involved in many signalling pathways including energy metabolism, body pattern formation and microtubule growth and stability (which aids axon growth). GSK3 beta is also thought to be involved in oncogenesis and the hormonal control of several regulatory proteins including glycogen synthase.

Phosphorylation of serine-9 inhibits GSK3 beta function.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Dog Expected from Sequence
Primate Expected from Sequence
Xenopus Expected from Sequence
Human Expected from Sequence
Bovine Expected from Sequence
Chicken Expected from Sequence
Zebrafish Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to rat GSK3 beta (pSer9) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM Hepes pH7.5
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
50% Glycerol
0.01%Bovine Serum Albumin
Immunogen
Phosphopeptide corresponding to amino acid sequence surrounding phoshorylated seine 9 of rat GSK3 beta.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P18266 Related reagents
Entrez Gene
Gsk3b Related reagents
GO Terms
GO:0001837 epithelial to mesenchymal transition
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0016055 Wnt receptor signaling pathway
GO:0005178 integrin binding
GO:0005524 ATP binding
GO:0005625 soluble fraction
GO:0005634 nucleus
GO:0006468 protein phosphorylation
GO:0006917 induction of apoptosis
GO:0030010 establishment of cell polarity
GO:0042493 response to drug
GO:0010226 response to lithium ion
GO:0019901 protein kinase binding
GO:0030877 beta-catenin destruction complex
GO:0034747 Axin-APC-beta-catenin-GSK3B complex
GO:0035255 ionotropic glutamate receptor binding
GO:0043197 dendritic spine
GO:0043407 negative regulation of MAP kinase activity
GO:0045121 membrane raft
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0048156 tau protein binding
GO:0048168 regulation of neuronal synaptic plasticity
GO:0050321 tau-protein kinase activity
GO:0050774 negative regulation of dendrite morphogenesis
Regulatory
For research purposes only

Applications of GSK3 Beta (pSer9) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 1/1000
  1. 1 For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1233 detects a band of approximately 46kDa in rat cortex cell lysates.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

References for GSK3 Beta (pSer9) antibody

  1. Green, J.B. (2004) Lkb1 and GSK3-beta: kinases at the center and poles of the action.
    Cell Cycle. 3 (1): 12-4.
  2. Papkoff, J. et al. (1998) WNT-1 and HGF regulate GSK3 beta activity and beta-catenin signaling in mammary epithelial cells.
    Biochem. Biophys. Res. Commun. 247:851-8.
  3. Stambolic, V. et al. (1994) Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation.
    Biochem, J. 303:701-4.
  4. Garrido, J. et al. (2007) GSK3 alpha and GSK3 beta are necessary for axon formation.
    FEBS Lett. 581:1579-86.
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