GSK3 Beta (pSer9) antibody

Rabbit anti GSK3 Beta (pSer9)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
GSK3 Beta (pSer9)

Product Code Applications Pack Size List Price Your Price Qty
AHP1233
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 ml loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rabbit anti Rat GSK3 beta (pSer9) antibody recognizes GSK3 beta, also known as glycogen synthase kinase-3 beta (GSK-3 beta), factor A (FA) and serine/threonine-protein kinase GSK3B, when phosphorylated at serine 9. GSK3 is a ~46 kDa member of the serine/threonine protein kinase family. Two isforms of GSK3 exist, alpha and beta. GSK3 beta is expressed in the testis, thymus, prostate, and ovary as well as being expressed at low levels in lung, brain and kidney tissue.

GSK3 beta is involved in many signalling pathways including energy metabolism, body pattern formation and microtubule growth and stability (which aids axon growth). GSK3 beta is also thought to be involved in oncogenesis and the hormonal control of several regulatory proteins including glycogen synthase.

Phosphorylation of serine-9 inhibits GSK3 beta function.

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Dog Expected from Sequence
Primate Expected from Sequence
Xenopus Expected from Sequence
Human Expected from Sequence
Bovine Expected from Sequence
Chicken Expected from Sequence
Zebrafish Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to rat GSK3 beta (pSer9) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM Hepes pH7.5
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
50% Glycerol
0.01%Bovine Serum Albumin
Immunogen
Phosphopeptide corresponding to amino acid sequence surrounding phoshorylated seine 9 of rat GSK3 beta.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1233 detects a band of approximately 46kDa in rat cortex cell lysates.

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References for GSK3 Beta (pSer9) antibody

  1. Green, J.B. (2004) Lkb1 and GSK3-beta: kinases at the center and poles of the action.
    Cell Cycle. 3 (1): 12-4.
  2. Papkoff, J. et al. (1998) WNT-1 and HGF regulate GSK3 beta activity and beta-catenin signaling in mammary epithelial cells.
    Biochem. Biophys. Res. Commun. 247:851-8.
  3. Stambolic, V. et al. (1994) Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation.
    Biochem, J. 303:701-4.
  4. Garrido, J. et al. (2007) GSK3 alpha and GSK3 beta are necessary for axon formation.
    FEBS Lett. 581:1579-86.

RRID
AB_877475
UniProt
P18266
Entrez Gene
Gsk3b
GO Terms
GO:0001837 epithelial to mesenchymal transition
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0016055 Wnt receptor signaling pathway
GO:0005178 integrin binding
GO:0005524 ATP binding
GO:0005625 soluble fraction
GO:0005634 nucleus
GO:0006468 protein phosphorylation
GO:0006917 induction of apoptosis
GO:0030010 establishment of cell polarity
GO:0042493 response to drug
GO:0010226 response to lithium ion
GO:0019901 protein kinase binding
GO:0030877 beta-catenin destruction complex
GO:0034747 Axin-APC-beta-catenin-GSK3B complex
GO:0035255 ionotropic glutamate receptor binding
GO:0043197 dendritic spine
GO:0043407 negative regulation of MAP kinase activity
GO:0045121 membrane raft
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0048156 tau protein binding
GO:0048168 regulation of neuronal synaptic plasticity
GO:0050321 tau-protein kinase activity
GO:0050774 negative regulation of dendrite morphogenesis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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