GSK3 Beta (pSer9) antibody
Rabbit anti Rat GSK3 beta (pSer9) antibody recognizes GSK3 beta, also known as glycogen synthase kinase-3 beta (GSK-3 beta), factor A (FA) and serine/threonine-protein kinase GSK3B, when phosphorylated at serine 9. GSK3 is a ~46 kDa member of the serine/threonine protein kinase family. Two isforms of GSK3 exist, alpha and beta. GSK3 beta is expressed in the testis, thymus, prostate, and ovary as well as being expressed at low levels in lung, brain and kidney tissue.
GSK3 beta is involved in many signalling pathways including energy metabolism, body pattern formation and microtubule growth and stability (which aids axon growth). GSK3 beta is also thought to be involved in oncogenesis and the hormonal control of several regulatory proteins including glycogen synthase.
Phosphorylation of serine-9 inhibits GSK3 beta function.
GSK3 beta is involved in many signalling pathways including energy metabolism, body pattern formation and microtubule growth and stability (which aids axon growth). GSK3 beta is also thought to be involved in oncogenesis and the hormonal control of several regulatory proteins including glycogen synthase.
Phosphorylation of serine-9 inhibits GSK3 beta function.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Dog Expected from Sequence Primate Expected from Sequence Xenopus Expected from Sequence Human Expected from Sequence Bovine Expected from Sequence Chicken Expected from Sequence Zebrafish Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to rat GSK3 beta (pSer9) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- 10mM Hepes pH7.5
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 50% Glycerol 0.01% Bovine Serum Albumin - Immunogen
- Phosphopeptide corresponding to amino acid sequence surrounding phoshorylated seine 9 of rat GSK3 beta.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch.
More Information
- UniProt
- P18266 Related reagents
- Entrez Gene
- Gsk3b Related reagents
- GO Terms
- GO:0001837 epithelial to mesenchymal transition
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0016055 Wnt receptor signaling pathway
- GO:0005178 integrin binding
- GO:0005524 ATP binding
- GO:0005625 soluble fraction
- GO:0005634 nucleus
- GO:0006468 protein phosphorylation
- GO:0006917 induction of apoptosis
- GO:0030010 establishment of cell polarity
- GO:0042493 response to drug
- GO:0010226 response to lithium ion
- GO:0019901 protein kinase binding
- GO:0030877 beta-catenin destruction complex
- GO:0034747 Axin-APC-beta-catenin-GSK3B complex
- GO:0035255 ionotropic glutamate receptor binding
- GO:0043197 dendritic spine
- GO:0043407 negative regulation of MAP kinase activity
- GO:0045121 membrane raft
- GO:0045892 negative regulation of transcription, DNA-dependent
- GO:0048156 tau protein binding
- GO:0048168 regulation of neuronal synaptic plasticity
- GO:0050321 tau-protein kinase activity
- GO:0050774 negative regulation of dendrite morphogenesis
- Regulatory
- For research purposes only
Applications of GSK3 Beta (pSer9) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1233 detects a band of approximately 46kDa in rat cortex cell lysates.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | C E P WB | 1 ml |
|
|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Product Specific References
References for GSK3 Beta (pSer9) antibody
-
Green, J.B. (2004) Lkb1 and GSK3-beta: kinases at the center and poles of the action.
Cell Cycle. 3 (1): 12-4. -
Papkoff, J. et al. (1998) WNT-1 and HGF regulate GSK3 beta activity and beta-catenin signaling in mammary epithelial cells.
Biochem. Biophys. Res. Commun. 247:851-8. -
Stambolic, V. et al. (1994) Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation.
Biochem, J. 303:701-4. -
Garrido, J. et al. (2007) GSK3 alpha and GSK3 beta are necessary for axon formation.
FEBS Lett. 581:1579-86.
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