GSK3 Beta (pSer9) antibody

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Rabbit anti GSK3 Beta (pSer9)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP1233 Datasheet	WB SDS	0.1 ml	Log in		Get Quote

Rabbit anti Rat GSK3 beta (pSer9) antibody recognizes GSK3 beta, also known as glycogen synthase kinase-3 beta (GSK-3 beta), factor A (FA) and serine/threonine-protein kinase GSK3B, when phosphorylated at serine 9. GSK3 is a ~46 kDa member of the serine/threonine protein kinase family. Two isforms of GSK3 exist, alpha and beta. GSK3 beta is expressed in the testis, thymus, prostate, and ovary as well as being expressed at low levels in lung, brain and kidney tissue.

GSK3 beta is involved in many signalling pathways including energy metabolism, body pattern formation and microtubule growth and stability (which aids axon growth). GSK3 beta is also thought to be involved in oncogenesis and the hormonal control of several regulatory proteins including glycogen synthase.

Phosphorylation of serine-9 inhibits GSK3 beta function.

Product Details

Target Species

Rat

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse	Expected from Sequence
Dog	Expected from Sequence
Primate	Expected from Sequence
Xenopus	Expected from Sequence
Human	Expected from Sequence
Bovine	Expected from Sequence
Chicken	Expected from Sequence
Zebrafish	Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to rat GSK3 beta (pSer9) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.

Buffer Solution

10mM Hepes pH7.5

Preservative Stabilisers

0.09%	Sodium Azide (NaN₃)
50%	Glycerol
0.01%	Bovine Serum Albumin

Immunogen

Phosphopeptide corresponding to amino acid sequence surrounding phoshorylated seine 9 of rat GSK3 beta.

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P18266
Entrez Gene: Gsk3b
GO Terms: GO:0001837 epithelial to mesenchymal transition; GO:0005829 cytosol; GO:0004674 protein serine/threonine kinase activity; GO:0016055 Wnt receptor signaling pathway; GO:0005178 integrin binding; GO:0005524 ATP binding; GO:0005625 soluble fraction; GO:0005634 nucleus; GO:0006468 protein phosphorylation; GO:0006917 induction of apoptosis; GO:0030010 establishment of cell polarity; GO:0042493 response to drug; GO:0010226 response to lithium ion; GO:0019901 protein kinase binding; GO:0030877 beta-catenin destruction complex; GO:0034747 Axin-APC-beta-catenin-GSK3B complex; GO:0035255 ionotropic glutamate receptor binding; GO:0043197 dendritic spine; GO:0043407 negative regulation of MAP kinase activity; GO:0045121 membrane raft; GO:0045892 negative regulation of transcription, DNA-dependent; GO:0048156 tau protein binding; GO:0048168 regulation of neuronal synaptic plasticity; GO:0050321 tau-protein kinase activity; GO:0050774 negative regulation of dendrite morphogenesis
Regulatory: For research purposes only

Applications of GSK3 Beta (pSer9) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1233 detects a band of approximately 46kDa in rat cortex cell lysates.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:FITC	STAR34B	C F	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	F IF WB	0.1 mg	Log in	Get Quote

Product Specific References

References for GSK3 Beta (pSer9) antibody

Green, J.B. (2004) Lkb1 and GSK3-beta: kinases at the center and poles of the action.
Cell Cycle. 3 (1): 12-4.
Papkoff, J. et al. (1998) WNT-1 and HGF regulate GSK3 beta activity and beta-catenin signaling in mammary epithelial cells.
Biochem. Biophys. Res. Commun. 247:851-8.
Stambolic, V. et al. (1994) Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation.
Biochem, J. 303:701-4.
Garrido, J. et al. (2007) GSK3 alpha and GSK3 beta are necessary for axon formation.
FEBS Lett. 581:1579-86.

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