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Glycine Receptor Alpha 1/2 antibody

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Anti Glycine Receptor Alpha 1/2 (N-Terminal) Antibody gallery image 1
Western blot of rat spinal cord and hippocampal lysates showing specific immunolabeling of the ~48 kDa glycine receptor in spinal cord using Rabbit anti Rat glycine receptor alpha 1/2 antibody (AHP929).

Rabbit anti Glycine Receptor Alpha 1/2 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP929 C WB 0.2 mg
Rabbit anti glycine receptor recognises an epitope within the N-terminal region (NT) of glycine receptor alpha 1 and alpha 2 subunits.

Glycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission. The receptors are pentameric membrane proteins which form chloride channels. Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation.

Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1 ml sterile PBS
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antisera to rat glycine receptor (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Ammonium bicarbonate
Preservative Stabilisers
None present
Immunogen
Synthetic peptide corresponding to the N-terminal region of rat glycine receptor alpha 1 subunit.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P07727 Related reagents
P22771 Related reagents
Entrez Gene
Glra1 Related reagents
Glra2 Related reagents
GO Terms
GO:0001964 startle response
GO:0006936 muscle contraction
GO:0004872 receptor activity
GO:0005783 endoplasmic reticulum
GO:0005887 integral to plasma membrane
GO:0005624 membrane fraction
GO:0007218 neuropeptide signaling pathway
GO:0016594 glycine binding
GO:0009897 external side of plasma membrane
GO:0016934 extracellular-glycine-gated chloride channel activity
GO:0030054 cell junction
GO:0030977 taurine binding
GO:0034707 chloride channel complex
GO:0051260 protein homooligomerization
GO:0045211 postsynaptic membrane
GO:0051291 protein heterooligomerization
GO:0051970 negative regulation of transmission of nerve impulse
GO:0060012 synaptic transmission, glycinergic
GO:0007416 synapse assembly
Regulatory
For research purposes only

Applications of Glycine Receptor Alpha 1/2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/1000
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP929 detects a band of approximately 48 kDa in rat spinal cord lysates.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for Glycine Receptor Alpha 1/2 antibody

  1. Chen, Z. et al. (2004) Molecular determinants of proton modulation of glycine receptors.
    J Biol Chem. 279 (2): 876-83.
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