Rabbit anti glycine receptor recognises an epitope within the N-terminal region (NT) of glycine receptor alpha 1 and alpha 2 subunits.
Glycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission. The receptors are pentameric membrane proteins which form chloride channels. Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation.
Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - lyophilized
- Reconstitute with 0.1 ml sterile PBS
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
- Antiserum Preparation
- Antisera to rat glycine receptor (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Ammonium bicarbonate
- Preservative Stabilisers
- None present
- Synthetic peptide corresponding to the N-terminal region of rat glycine receptor alpha 1 subunit.
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to plasma membrane
neuropeptide signaling pathway
external side of plasma membrane
extracellular-glycine-gated chloride channel activity
chloride channel complex
negative regulation of transmission of nerve impulse
synaptic transmission, glycinergic
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Glycine Receptor Alpha 1/2 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP929 detects a band of approximately 48 kDa in rat spinal cord lysates.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for Glycine Receptor Alpha 1/2 antibody
Chen, Z. et al. (2004) Molecular determinants of proton modulation of glycine receptors.
J Biol Chem. 279 (2): 876-83.
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