Glycine Receptor Alpha 1/2 antibody
Rabbit anti glycine receptor recognises an epitope within the N-terminal region (NT) of glycine receptor alpha 1 and alpha 2 subunits.
Glycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission. The receptors are pentameric membrane proteins which form chloride channels. Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation.
Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain.
Glycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission. The receptors are pentameric membrane proteins which form chloride channels. Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation.
Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.1 ml sterile PBS
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antisera to rat glycine receptor (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Ammonium bicarbonate
- Preservative Stabilisers
- None present
- Immunogen
- Synthetic peptide corresponding to the N-terminal region of rat glycine receptor alpha 1 subunit.
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P07727 Related reagents
- P22771 Related reagents
- Entrez Gene
- Glra1 Related reagents
- Glra2 Related reagents
- GO Terms
- GO:0001964 startle response
- GO:0006936 muscle contraction
- GO:0004872 receptor activity
- GO:0005783 endoplasmic reticulum
- GO:0005887 integral to plasma membrane
- GO:0005624 membrane fraction
- GO:0007218 neuropeptide signaling pathway
- GO:0016594 glycine binding
- GO:0009897 external side of plasma membrane
- GO:0016934 extracellular-glycine-gated chloride channel activity
- GO:0030054 cell junction
- GO:0030977 taurine binding
- GO:0034707 chloride channel complex
- GO:0051260 protein homooligomerization
- GO:0045211 postsynaptic membrane
- GO:0051291 protein heterooligomerization
- GO:0051970 negative regulation of transmission of nerve impulse
- GO:0060012 synaptic transmission, glycinergic
- GO:0007416 synapse assembly
- Regulatory
- For research purposes only
Applications of Glycine Receptor Alpha 1/2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | 1/1000 | ||
| Western Blotting | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP929 detects a band of approximately 48 kDa in rat spinal cord lysates.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
References for Glycine Receptor Alpha 1/2 antibody
-
Chen, Z. et al. (2004) Molecular determinants of proton modulation of glycine receptors.
J Biol Chem. 279 (2): 876-83.
