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GluR1 (pSer831) antibody

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Anti GluR1 (pSer831) Antibody gallery image 1
Western blot of rat hippocampal lysate showing phosphospecific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser831 using Rabbit anti Rat GluR1 (pSer831) antibody (AHP914).

Rabbit anti GluR1 (pSer831)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP914
Datasheet
WB
MSDS
0.15 ml loader

Rabbit anti Rat GluR1 (pSer831) antibody recognizes glutamate receptor 1 (GluR1), also known as AMPA-selective glutamate receptor 1, GluR-A, GluR-K1 and glutamate receptor ionotropic , AMPA 1 (GluA1), when phosphorylated at serine 831.

GluR1, widely expressed throughout the nervous system is a component of AMPA receptors (alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid receptors), which play a key role at excitatory synapses.

Phosphorylation of Ser831 and Ser845 is reported to potentiate GluR1 function, suggesting a possible role in synaptic plasticity, learning and memory.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Dog Expected from Sequence
Human Expected from Sequence
Chicken Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to phosphorylated GluR1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by sequential affinity chromatography on phospho- and dephosphopeptide affinity columns.
Buffer Solution
10mM HEPES pH7.5
Preservative Stabilisers
0.09%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
Synthetic phosphopeptide corresponding to amino acid sequence within GluR1 which includes the phosphorylated residue Ser831.

Storage Information

Storage
Store at +4oC or -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P19490
Entrez Gene
Gria1
GO Terms
GO:0001919 regulation of receptor recycling
GO:0001965 G-protein alpha-subunit binding
GO:0005829 cytosol
GO:0004970 ionotropic glutamate receptor activity
GO:0051018 protein kinase A binding
GO:0005234 extracellular-glutamate-gated ion channel activity
GO:0005769 early endosome
GO:0005789 endoplasmic reticulum membrane
GO:0007616 long-term memory
GO:0008179 adenylate cyclase binding
GO:0008328 ionotropic glutamate receptor complex
GO:0042493 response to drug
GO:0010226 response to lithium ion
GO:0014069 postsynaptic density
GO:0046685 response to arsenic-containing substance
GO:0019228 regulation of action potential in neuron
GO:0019717 synaptosome
GO:0019901 protein kinase binding
GO:0021510 spinal cord development
GO:0030054 cell junction
GO:0030165 PDZ domain binding
GO:0031267 small GTPase binding
GO:0031489 myosin V binding
GO:0031594 neuromuscular junction
GO:0031623 receptor internalization
GO:0031681 G-protein beta-subunit binding
GO:0031698 beta-2 adrenergic receptor binding
GO:0032279 asymmetric synapse
GO:0032355 response to estradiol stimulus
GO:0035249 synaptic transmission, glutamatergic
GO:0042220 response to cocaine
GO:0042803 protein homodimerization activity
GO:0043025 neuronal cell body
GO:0043197 dendritic spine
GO:0043198 dendritic shaft
GO:0045211 postsynaptic membrane
GO:0045838 positive regulation of membrane potential
GO:0048167 regulation of synaptic plasticity
GO:0050806 positive regulation of synaptic transmission
GO:0051602 response to electrical stimulus
GO:0060992 response to fungicide
GO:0071230 cellular response to amino acid stimulus
GO:0071359 cellular response to dsRNA
GO:0071363 cellular response to growth factor stimulus
GO:0071375 cellular response to peptide hormone stimulus
GO:0071407 cellular response to organic cyclic compound
GO:0071445 cellular response to protein stimulus
Regulatory
For research purposes only

Applications of GluR1 (pSer831) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP914 detects a band of approximately 100 kDa in rat brain lysates.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Product Specific References

Further Reading

  1. Castellani, G.C. et al. (2001) A biophysical model of bidirectional synaptic plasticity: Dependence on AMPA and NMDA receptors.
    Proc. Natl. Acad. Sci. USA. 98: 12772 - 12777.
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