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GluR1 antibody

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Rabbit anti Rat Glutamate Receptor 1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
4670-5107
Datasheet
C IP WB
SDS
50 µg loader

Rabbit anti Rat Glutamate Receptor 1 (C-Terminal) antibody recognizes the glutamate receptor 1 (also known as AMPA) which acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

This antibody linked to immobilized Protein A immunoprecipitates detergent-solubilized receptor from brain or transfected cells.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Hamster
Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified Ig - liquid
Preparation
Purified Ig prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3
Immunogen
Synthetic peptide corresponding to the C terminal region (SHSSGMPLGATGL) of the native molecule conjugated to Bovine Serum Albumin.
Approx. Protein Concentrations
Ig concentration 1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P19490
Entrez Gene
Gria1
GO Terms
GO:0001919 regulation of receptor recycling
GO:0001965 G-protein alpha-subunit binding
GO:0005829 cytosol
GO:0004970 ionotropic glutamate receptor activity
GO:0051018 protein kinase A binding
GO:0005234 extracellular-glutamate-gated ion channel activity
GO:0005769 early endosome
GO:0005789 endoplasmic reticulum membrane
GO:0007616 long-term memory
GO:0008179 adenylate cyclase binding
GO:0008328 ionotropic glutamate receptor complex
GO:0042493 response to drug
GO:0010226 response to lithium ion
GO:0014069 postsynaptic density
GO:0046685 response to arsenic-containing substance
GO:0019228 regulation of action potential in neuron
GO:0019717 synaptosome
GO:0019901 protein kinase binding
GO:0021510 spinal cord development
GO:0030054 cell junction
GO:0030165 PDZ domain binding
GO:0031267 small GTPase binding
GO:0031489 myosin V binding
GO:0031594 neuromuscular junction
GO:0031623 receptor internalization
GO:0031681 G-protein beta-subunit binding
GO:0031698 beta-2 adrenergic receptor binding
GO:0032279 asymmetric synapse
GO:0032355 response to estradiol stimulus
GO:0035249 synaptic transmission, glutamatergic
GO:0042220 response to cocaine
GO:0042803 protein homodimerization activity
GO:0043025 neuronal cell body
GO:0043197 dendritic spine
GO:0043198 dendritic shaft
GO:0045211 postsynaptic membrane
GO:0045838 positive regulation of membrane potential
GO:0048167 regulation of synaptic plasticity
GO:0050806 positive regulation of synaptic transmission
GO:0051602 response to electrical stimulus
GO:0060992 response to fungicide
GO:0071230 cellular response to amino acid stimulus
GO:0071359 cellular response to dsRNA
GO:0071363 cellular response to growth factor stimulus
GO:0071375 cellular response to peptide hormone stimulus
GO:0071407 cellular response to organic cyclic compound
GO:0071445 cellular response to protein stimulus
Regulatory
For research purposes only

Applications of GluR1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1 µg/ml 3 µg/ml
Immunoprecipitation
Western Blotting 0.5 µg/ml 2.5 µg/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Immunohistology
Paraformaldehyde or paraformaldehyde/glutaraldehyde are suitable. Cryostat and vibratome sections can be used with or without Triton X-100 treatment.
Western Blotting
Western blot analysis of rat brain shows a single band of approximately 110 kDa co-migrating with GluR1 expressed in transfected cells.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

References for GluR1 antibody

  1. Petralia, R.S. & Wenthold, R.J. (1992) Light and electron immunocytochemical localization of AMPA-selective glutamate receptors in the rat brain.
    J Comp Neurol. 318 (3): 329-54.
  2. Wenthold, R.J. et al. (1992) Immunochemical characterization of the non-NMDA glutamate receptor using subunit-specific antibodies. Evidence for a hetero-oligomeric structure in rat brain.
    J Biol Chem. 267 (1): 501-7.

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