GluR1 antibody

This antibody linked to immobilized Protein A immunoprecipitates detergent-solubilized receptor from brain or transfected cells.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Hamster Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified Ig - liquid
- Preparation
- Purified Ig prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3
- Immunogen
- Synthetic peptide corresponding to the C terminal region (SHSSGMPLGATGL) of the native molecule conjugated to Bovine Serum Albumin.
- Approx. Protein Concentrations
- Ig concentration 1 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P19490
- Entrez Gene
- Gria1
- GO Terms
- GO:0001919 regulation of receptor recycling
- GO:0001965 G-protein alpha-subunit binding
- GO:0005829 cytosol
- GO:0004970 ionotropic glutamate receptor activity
- GO:0051018 protein kinase A binding
- GO:0005234 extracellular-glutamate-gated ion channel activity
- GO:0005769 early endosome
- GO:0005789 endoplasmic reticulum membrane
- GO:0007616 long-term memory
- GO:0008179 adenylate cyclase binding
- GO:0008328 ionotropic glutamate receptor complex
- GO:0042493 response to drug
- GO:0010226 response to lithium ion
- GO:0014069 postsynaptic density
- GO:0046685 response to arsenic-containing substance
- GO:0019228 regulation of action potential in neuron
- GO:0019717 synaptosome
- GO:0019901 protein kinase binding
- GO:0021510 spinal cord development
- GO:0030054 cell junction
- GO:0030165 PDZ domain binding
- GO:0031267 small GTPase binding
- GO:0031489 myosin V binding
- GO:0031594 neuromuscular junction
- GO:0031623 receptor internalization
- GO:0031681 G-protein beta-subunit binding
- GO:0031698 beta-2 adrenergic receptor binding
- GO:0032279 asymmetric synapse
- GO:0032355 response to estradiol stimulus
- GO:0035249 synaptic transmission, glutamatergic
- GO:0042220 response to cocaine
- GO:0042803 protein homodimerization activity
- GO:0043025 neuronal cell body
- GO:0043197 dendritic spine
- GO:0043198 dendritic shaft
- GO:0045211 postsynaptic membrane
- GO:0045838 positive regulation of membrane potential
- GO:0048167 regulation of synaptic plasticity
- GO:0050806 positive regulation of synaptic transmission
- GO:0051602 response to electrical stimulus
- GO:0060992 response to fungicide
- GO:0071230 cellular response to amino acid stimulus
- GO:0071359 cellular response to dsRNA
- GO:0071363 cellular response to growth factor stimulus
- GO:0071375 cellular response to peptide hormone stimulus
- GO:0071407 cellular response to organic cyclic compound
- GO:0071445 cellular response to protein stimulus
- Regulatory
- For research purposes only
Applications of GluR1 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Frozen | 1 µg/ml | 3 µg/ml | |
Immunoprecipitation | |||
Western Blotting | 0.5 µg/ml | 2.5 µg/ml |
- Immunohistology
- Paraformaldehyde or paraformaldehyde/glutaraldehyde are suitable. Cryostat and vibratome sections can be used with or without Triton X-100 treatment.
- Western Blotting
- Western blot analysis of rat brain shows a single band of approximately 110 kDa co-migrating with GluR1 expressed in transfected cells.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for GluR1 antibody
-
Petralia, R.S. & Wenthold, R.J. (1992) Light and electron immunocytochemical localization of AMPA-selective glutamate receptors in the rat brain.
J Comp Neurol. 318 (3): 329-54. -
Wenthold, R.J. et al. (1992) Immunochemical characterization of the non-NMDA glutamate receptor using subunit-specific antibodies. Evidence for a hetero-oligomeric structure in rat brain.
J Biol Chem. 267 (1): 501-7.
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