GluR1 antibody

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Rabbit anti Rat Glutamate Receptor 1 (C-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
4670-5107 Datasheet	C IP WB SDS	50 µg	Log in		Get Quote

Rabbit anti Rat Glutamate Receptor 1 (C-Terminal) antibody recognizes the glutamate receptor 1 (also known as AMPA) which acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

This antibody linked to immobilized Protein A immunoprecipitates detergent-solubilized receptor from brain or transfected cells.

Product Details

Target Species

Rat

Species Cross-Reactivity

Target Species	Cross Reactivity
Human
Hamster
Monkey

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified Ig - liquid

Preparation

Purified Ig prepared by affinity chromatography

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

<0.1% Sodium Azide (NaN₃)

Immunogen

Synthetic peptide corresponding to the C terminal region (SHSSGMPLGATGL) of the native molecule conjugated to Bovine Serum Albumin.

Approx. Protein Concentrations

Ig concentration 1 mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P19490
Entrez Gene: Gria1
GO Terms: GO:0001919 regulation of receptor recycling; GO:0001965 G-protein alpha-subunit binding; GO:0005829 cytosol; GO:0004970 ionotropic glutamate receptor activity; GO:0051018 protein kinase A binding; GO:0005234 extracellular-glutamate-gated ion channel activity; GO:0005769 early endosome; GO:0005789 endoplasmic reticulum membrane; GO:0007616 long-term memory; GO:0008179 adenylate cyclase binding; GO:0008328 ionotropic glutamate receptor complex; GO:0042493 response to drug; GO:0010226 response to lithium ion; GO:0014069 postsynaptic density; GO:0046685 response to arsenic-containing substance; GO:0019228 regulation of action potential in neuron; GO:0019717 synaptosome; GO:0019901 protein kinase binding; GO:0021510 spinal cord development; GO:0030054 cell junction; GO:0030165 PDZ domain binding; GO:0031267 small GTPase binding; GO:0031489 myosin V binding; GO:0031594 neuromuscular junction; GO:0031623 receptor internalization; GO:0031681 G-protein beta-subunit binding; GO:0031698 beta-2 adrenergic receptor binding; GO:0032279 asymmetric synapse; GO:0032355 response to estradiol stimulus; GO:0035249 synaptic transmission, glutamatergic; GO:0042220 response to cocaine; GO:0042803 protein homodimerization activity; GO:0043025 neuronal cell body; GO:0043197 dendritic spine; GO:0043198 dendritic shaft; GO:0045211 postsynaptic membrane; GO:0045838 positive regulation of membrane potential; GO:0048167 regulation of synaptic plasticity; GO:0050806 positive regulation of synaptic transmission; GO:0051602 response to electrical stimulus; GO:0060992 response to fungicide; GO:0071230 cellular response to amino acid stimulus; GO:0071359 cellular response to dsRNA; GO:0071363 cellular response to growth factor stimulus; GO:0071375 cellular response to peptide hormone stimulus; GO:0071407 cellular response to organic cyclic compound; GO:0071445 cellular response to protein stimulus
Regulatory: For research purposes only

Applications of GluR1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunohistology - Frozen	1 µg/ml	3 µg/ml
Immunoprecipitation
Western Blotting	0.5 µg/ml	2.5 µg/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Immunohistology: Paraformaldehyde or paraformaldehyde/glutaraldehyde are suitable. Cryostat and vibratome sections can be used with or without Triton X-100 treatment.
Western Blotting: Western blot analysis of rat brain shows a single band of approximately 110 kDa co-migrating with GluR1 expressed in transfected cells.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:FITC	STAR34B	C F	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	F IF WB	0.1 mg	Log in	Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in		Get Quote

Product Specific References

References for GluR1 antibody

Petralia, R.S. & Wenthold, R.J. (1992) Light and electron immunocytochemical localization of AMPA-selective glutamate receptors in the rat brain.
J Comp Neurol. 318 (3): 329-54.
Wenthold, R.J. et al. (1992) Immunochemical characterization of the non-NMDA glutamate receptor using subunit-specific antibodies. Evidence for a hetero-oligomeric structure in rat brain.
J Biol Chem. 267 (1): 501-7.

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