Rabbit anti Rat Glutamate Receptor 1 (C-Terminal) antibody recognizes the glutamate receptor 1 (also known as AMPA) which acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
This antibody linked to immobilized Protein A immunoprecipitates detergent-solubilized receptor from brain or transfected cells.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified Ig - liquid
- Purified Ig prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3
- Synthetic peptide corresponding to the C terminal region (SHSSGMPLGATGL) of the native molecule conjugated to Bovine Serum Albumin.
- Approx. Protein Concentrations
- Ig concentration 1 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch.
- Entrez Gene
- GO Terms
regulation of receptor recycling
G-protein alpha-subunit binding
ionotropic glutamate receptor activity
protein kinase A binding
extracellular-glutamate-gated ion channel activity
endoplasmic reticulum membrane
adenylate cyclase binding
ionotropic glutamate receptor complex
response to drug
response to lithium ion
response to arsenic-containing substance
regulation of action potential in neuron
protein kinase binding
spinal cord development
PDZ domain binding
small GTPase binding
myosin V binding
G-protein beta-subunit binding
beta-2 adrenergic receptor binding
response to estradiol stimulus
synaptic transmission, glutamatergic
response to cocaine
protein homodimerization activity
neuronal cell body
positive regulation of membrane potential
regulation of synaptic plasticity
positive regulation of synaptic transmission
response to electrical stimulus
response to fungicide
cellular response to amino acid stimulus
cellular response to dsRNA
cellular response to growth factor stimulus
cellular response to peptide hormone stimulus
cellular response to organic cyclic compound
cellular response to protein stimulus
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of GluR1 antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Paraformaldehyde or paraformaldehyde/glutaraldehyde are suitable. Cryostat and vibratome sections can be used with or without Triton X-100 treatment.
- Western Blotting
- Western blot analysis of rat brain shows a single band of approximately 110 kDa co-migrating with GluR1 expressed in transfected cells.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for GluR1 antibody
Petralia, R.S. & Wenthold, R.J. (1992) Light and electron immunocytochemical localization of AMPA-selective glutamate receptors in the rat brain.
J Comp Neurol. 318 (3): 329-54.
Wenthold, R.J. et al. (1992) Immunochemical characterization of the non-NMDA glutamate receptor using subunit-specific antibodies. Evidence for a hetero-oligomeric structure in rat brain.
J Biol Chem. 267 (1): 501-7.