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GAP43 (pSer41) antibody

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Anti GAP43 (pSer41) Antibody gallery image 1
Western blot of rat cortex lysate showing phosphospecific immunolabeling of the ~50 kDa GAP-43 protein phosphorylated at Ser41 using Rabbit anti Rat GAP43 (pSer41) antibody (AHP902).

Rabbit anti GAP43 (pSer41)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP902 WB* 0.1 ml
Rabbit anti Rat GAP43 (pSer41) antibody recognizes growth-associated protein 43 (GAP43), also known as neuromodulin, axonal membrane protein GAP-43, neural phosphoprotein B-50 and pp46, when phosphorylated at serine 41.

GAP-43 is expressed in neuronal growth cones during development and during axonal regeneration and is phosphorylated at Ser41 by protein kinase C.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human Expected from Sequence
Bovine Expected from Sequence
Chicken Expected from Sequence
Dog Expected from Sequence
Monkey Expected from Sequence
Xenopus Expected from Sequence
Zebrafish Expected from Sequence
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to phosphorylated GAP-43 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5
Preservative Stabilisers
0.09%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence within GAP-43 which includes phosphorylated Ser41.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P17677 Related reagents
Entrez Gene
GAP43 Related reagents
GO Terms
GO:0005516 calmodulin binding
GO:0007205 activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway
GO:0007399 nervous system development
GO:0009611 response to wounding
GO:0030054 cell junction
GO:0030154 cell differentiation
GO:0032584 growth cone membrane
GO:0040008 regulation of growth
GO:0045202 synapse
Regulatory
For research purposes only

Applications of GAP43 (pSer41) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 1/1000
  1. 1 For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP902 detects a band of approximately 50kDa in rat cortex lysates.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

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Description Product Code Pack Size Applications List Price Quantity
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