GAP43 (pSer41) antibody
Rabbit anti Rat GAP43 (pSer41) antibody recognizes growth-associated protein 43 (GAP43), also known as neuromodulin, axonal membrane protein GAP-43, neural phosphoprotein B-50 and pp46, when phosphorylated at serine 41.
GAP-43 is expressed in neuronal growth cones during development and during axonal regeneration and is phosphorylated at Ser41 by protein kinase C.
GAP-43 is expressed in neuronal growth cones during development and during axonal regeneration and is phosphorylated at Ser41 by protein kinase C.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Expected from Sequence Bovine Expected from Sequence Chicken Expected from Sequence Dog Expected from Sequence Monkey Expected from Sequence Xenopus Expected from Sequence Zebrafish Expected from Sequence Mouse Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to phosphorylated GAP-43 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- 10mM HEPES pH7.5
- Preservative Stabilisers
0.09% Sodium Azide 0.01% Bovine Serum Albumin 50% Glycerol - Immunogen
- Synthetic phosphopeptide corresponding to an amino acid sequence within GAP-43 which includes phosphorylated Ser41.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch.
More Information
- UniProt
- P17677 Related reagents
- Entrez Gene
- GAP43 Related reagents
- GO Terms
- GO:0005516 calmodulin binding
- GO:0007205 activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway
- GO:0007399 nervous system development
- GO:0009611 response to wounding
- GO:0030054 cell junction
- GO:0030154 cell differentiation
- GO:0032584 growth cone membrane
- GO:0040008 regulation of growth
- GO:0045202 synapse
- Regulatory
- For research purposes only
Applications of GAP43 (pSer41) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting 1 | 1/1000 |
- 1 For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP902 detects a band of approximately 50kDa in rat cortex lysates.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
