Dynamin (pSer774) antibody
Sheep anti Rat dynamin (pSer774) antibody recognizes dynamin, also known as B-dynamin and D100, when phosphorylated at serine 774.
Dynamin is a phosphoprotein expressed at nerve terminals, which plays an important role in the regulation of synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 (cdk5) has been shown to phosphorylate serine 774 and 778 in vivo. Dephosphorylation of these residues is essential for endocytosis of synaptic vesicles.
Dynamin is a phosphoprotein expressed at nerve terminals, which plays an important role in the regulation of synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 (cdk5) has been shown to phosphorylate serine 774 and 778 in vivo. Dephosphorylation of these residues is essential for endocytosis of synaptic vesicles.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Expected from Sequence Monkey Expected from Sequence Bovine Expected from Sequence Chicken Expected from Sequence Mouse Expected from Sequence Dog Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to phosphorylated rat dynamin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- 10mM HEPES pH7.5
- Preservative Stabilisers
0.09% Sodium Azide 0.01% Bovine Serum Albumin 50% Glycerol - Immunogen
- Synthetic phosphopeptide corresponding to an amino acid sequence within dynamin which includes phosphorylated Ser774.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P21575
- Entrez Gene
- Dnm1
- GO Terms
- GO:0005886 plasma membrane
- GO:0003924 GTPase activity
- GO:0003774 motor activity
- GO:0005525 GTP binding
- GO:0005794 Golgi apparatus
- GO:0005874 microtubule
- GO:0008022 protein C-terminus binding
- GO:0042802 identical protein binding
- GO:0031623 receptor internalization
- GO:0032403 protein complex binding
- Regulatory
- For research purposes only
Applications of Dynamin (pSer774) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Western Blotting | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP899 detects a band of approximately 95kDa in rat hippocampus lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
|
Product Specific References
References for Dynamin (pSer774) antibody
-
Morton, A. et al. (2015) Synaptic vesicle exocytosis and increased cytosolic calcium are both necessary but not sufficient for activity-dependent bulk endocytosis.
J Neurochem. 134 (3): 405-15. -
Marland, J.R. et al. (2016) Synaptic Vesicle Recycling Is Unaffected in the Ts65Dn Mouse Model of Down Syndrome.
PLoS One. 11 (1): e0147974.
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