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Dynamin (pSer774) antibody

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Anti Dynamin (pSer774) Antibody gallery image 1
Western blot of a forskolin stimulated rat hippocampal lysate showing phosphospecific immunolabeling of the ~95 kDa Dynamin protein phosphorylated at Ser774 using Sheep anti Rat dynamin (pSer774) antibody (AHP899).

Sheep anti Dynamin (pSer774)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP899 C IF WB* 0.1 ml
Sheep anti Rat dynamin (pSer774) antibody recognizes dynamin, also known as B-dynamin and D100, when phosphorylated at serine 774.

Dynamin is a phosphoprotein expressed at nerve terminals, which plays an important role in the regulation of synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 (cdk5) has been shown to phosphorylate serine 774 and 778 in vivo. Dephosphorylation of these residues is essential for endocytosis of synaptic vesicles.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human Expected from Sequence
Monkey Expected from Sequence
Bovine Expected from Sequence
Chicken Expected from Sequence
Mouse Expected from Sequence
Dog Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to phosphorylated rat dynamin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5
Preservative Stabilisers
0.09%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence within dynamin which includes phosphorylated Ser774.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P21575 Related reagents
Entrez Gene
Dnm1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0003924 GTPase activity
GO:0003774 motor activity
GO:0005525 GTP binding
GO:0005794 Golgi apparatus
GO:0005874 microtubule
GO:0008022 protein C-terminus binding
GO:0042802 identical protein binding
GO:0031623 receptor internalization
GO:0032403 protein complex binding
Regulatory
For research purposes only

Applications of Dynamin (pSer774) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen
Western Blotting 1 1/1000
  1. 1 For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP899 detects a band of approximately 95kDa in rat hippocampus lysates.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Product Specific References

References for Dynamin (pSer774) antibody

  1. Morton, A. et al. (2015) Synaptic vesicle exocytosis and increased cytosolic calcium are both necessary but not sufficient for activity-dependent bulk endocytosis.
    J Neurochem. 134 (3): 405-15.
  2. Marland, J.R. et al. (2016) Synaptic Vesicle Recycling Is Unaffected in the Ts65Dn Mouse Model of Down Syndrome.
    PLoS One. 11 (1): e0147974.
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