CXCL1 antibody

Rabbit anti Rat CXCL1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CXCL1

Product Code Applications Pack Size List Price Your Price Qty
AAR22
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SDS Safety Datasheet SDS
E FN WB 0.1 mg loader
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Rabbit anti Rat CXCL1 antibody recognizes rat CXCL1 also known as GRO1 and CINC-1, an ~8 kDa cytokine which promotes neutrophil chemotaxis and degranulation and is expressed predominantly by monocytes after activation, but is also expressed by fibroblasts, endothelial cells, synovial cells and several tumour cell lines.

Target Species
Rat
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Preparation
Purified IgG prepared by affinity chromatography
Antiserum Preparation
Antiserum to CXCL1 was raised by repeated immunisation of rabbits with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant rat CXCL1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml 2.0ug/ml
Functional Assays 5ug/ml 10ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with the appropriate negative/positive controls.

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References for CXCL1 antibody

  1. Vallès, A. et al. (2006) Differential regulation of the CXCR2 chemokine network in rat brain trauma: implications forneuroimmune interactions and neuronal survival.
    Neurobiol Dis.22: 312-22.

Synonyms
GRO1
RRID
AB_2087721
UniProt
P14095
Entrez Gene
Cxcl1
GO Terms
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008009 chemokine activity
GO:0008083 growth factor activity
GO:0030593 neutrophil chemotaxis
View more products with CXCL1 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rat Products