Collagen III antibody

100% Secure

Rabbit anti Rat Collagen III

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2150-1948 C E IF P 50 µg
Rabbit anti Rat Collagen III antibody recognizes rat collagen type III, binding to both native and heat denatured collagen type III. This antibody is reactive with rat kidney, liver, skin and heart, but does not stain basement membranes.

The antiserum was cross-absorbed with immobilized rat serum proteins.

Product Details

Target Species
Rat
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antiserum to rat collagen type III was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on immobilized rat collagen type III.
Buffer Solution
Phosphate/Borate buffered saline.
Preservative Stabilisers
1% Mannitol
1% Dextran
Immunogen
Collagen type III from rat tail tendon.
Approx. Protein Concentrations
0.1 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P13941 Related reagents
Entrez Gene
Col3a1 Related reagents
GO Terms
GO:0001501 skeletal system development
GO:0005586 collagen type III
Regulatory
For research purposes only

Applications of Collagen III antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/3000
Immunofluorescence
Immunohistology - Frozen 1/20
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

References for Collagen III antibody

  1. Jones, H.B. et al. (2013) Quantitative histopathological assessment of retardation of islets of langerhans degeneration in rosiglitazone-dosed obese ZDF rats using combined insulin and collagens (I and III) immunohistochemistry with automated image analysis and statistical modeling.
    Toxicol Pathol. 41 (3): 425-44.
  2. Dankers P. Y.W. et al. (2015) Convenient formulation and application of a supramolecular ureido-pyrimidinone modified poly(ethylene glycol) carrier for intrarenal growth factor delivery
    European Polymer Journal. Jul 2 [Epub ahead of Print]