Collagen III antibody

Rabbit anti Rat Collagen III

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Collagen III

Product Code Applications Pack Size List Price Your Price Qty
2150-1948
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E IF P 50 µg
List Price Your Price
Search for Batch Specific Datasheets

Rabbit anti Rat Collagen III antibody recognizes rat collagen type III, binding to both native and heat denatured collagen type III. This antibody is reactive with rat kidney, liver, skin and heart, but does not stain basement membranes.

The antiserum was cross-absorbed with immobilized rat serum proteins.

Target Species
Rat
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antiserum to rat collagen type III was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on immobilized rat collagen type III.
Buffer Solution
0.1M Sodium Chloride
0.01M Sodium Phosphate
0.01M Sodium Borate
Preservative Stabilisers
1% Mannitol
1% Dextran
Immunogen
Collagen type III from rat tail tendon.
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/3000
Immunofluorescence
Immunohistology - Frozen 1/20
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):HRP
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:FITC
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:RPE

References for Collagen III antibody

  1. Jones, H.B. et al. (2013) Quantitative histopathological assessment of retardation of islets of langerhans degeneration in rosiglitazone-dosed obese ZDF rats using combined insulin and collagens (I and III) immunohistochemistry with automated image analysis and statistical modeling.
    Toxicol Pathol. 41 (3): 425-44.
  2. Dankers P. Y.W. et al. (2015) Convenient formulation and application of a supramolecular ureido-pyrimidinone modified poly(ethylene glycol) carrier for intrarenal growth factor delivery
    European Polymer Journal. Jul 2 [Epub ahead of Print]
  3. Silva, L.A. et al. (2017) Collagen concentration on the facial skin of postmenopausal women after topical treatment with estradiol and genistein: a randomized double-blind controlled trial.
    Gynecol Endocrinol. 33 (11): 845-8.

RRID
AB_620500
UniProt
P13941
Entrez Gene
Col3a1
GO Terms
GO:0001501 skeletal system development
GO:0005586 collagen type III
View more products with COLLAGEN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rat Products