Collagen III antibody
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Rabbit anti Rat Collagen III antibody recognizes rat collagen type III, binding to both native and heat denatured collagen type III. This antibody is reactive with rat kidney, liver, skin and heart, but does not stain basement membranes.
The antiserum was cross-absorbed with immobilized rat serum proteins.
The antiserum was cross-absorbed with immobilized rat serum proteins.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Antiserum Preparation
- Antiserum to rat collagen type III was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on immobilized rat collagen type III.
- Buffer Solution
- 0.15M Sodium Chloride
0.01M Sodium Phosphate - Preservative Stabilisers
- 1% Mannitol
1% Dextran - Immunogen
- Collagen type III from rat tail tendon.
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P13941
- Entrez Gene
- Col3a1
- GO Terms
- GO:0001501 skeletal system development
- GO:0005586 collagen type III
- Regulatory
- For research purposes only
Applications of Collagen III antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/3000 | ||
| Immunofluorescence | |||
| Immunohistology - Frozen | 1/20 | ||
| Immunohistology - Paraffin |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Product Specific References
References for Collagen III antibody
-
Jones, H.B. et al. (2013) Quantitative histopathological assessment of retardation of islets of langerhans degeneration in rosiglitazone-dosed obese ZDF rats using combined insulin and collagens (I and III) immunohistochemistry with automated image analysis and statistical modeling.
Toxicol Pathol. 41 (3): 425-44. -
Dankers P. Y.W. et al. (2015) Convenient formulation and application of a supramolecular ureido-pyrimidinone modified poly(ethylene glycol) carrier for intrarenal growth factor delivery
European Polymer Journal. Jul 2 [Epub ahead of Print] -
Silva, L.A. et al. (2017) Collagen concentration on the facial skin of postmenopausal women after topical treatment with estradiol and genistein: a randomized double-blind controlled trial.
Gynecol Endocrinol. 33 (11): 845-8.
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