Collagen III antibody
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Rabbit anti Rat Collagen III
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Collagen III
Antibody Quality and Performance Guarantee
| Rabbit anti Rat collagen III antibody recognizes rat collagen type III, binding to both native and heat denatured collagen type III. This antibody is reactive with rat kidney, liver, skin and heart, but does not stain basement membranes. The antiserum was cross-absorbed with immobilized rat serum proteins. |
- Target Species
- Rat
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Antiserum Preparation
- Antiserum to rat collagen type III was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on immobilized rat collagen type III.
- Buffer Solution
- 0.1M Sodium Chloride
0.01M Sodium Phosphate
0.01M Sodium Borate - Preservative Stabilisers
- 1% Mannitol
1% Dextran - Immunogen
- Collagen type III from rat tail tendon.
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/3000 | |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
1/20 | |
| Immunohistology - Paraffin | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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References for Collagen III antibody
-
Jones, H.B. et al. (2013) Quantitative histopathological assessment of retardation of islets of langerhans degeneration in rosiglitazone-dosed obese ZDF rats using combined insulin and collagens (I and III) immunohistochemistry with automated image analysis and statistical modeling.
Toxicol Pathol. 41 (3): 425-44. -
Dankers, P.Y.W. et al. (2015) Convenient formulation and application of a supramolecular ureido-pyrimidinone modified poly(ethylene glycol) carrier for intrarenal growth factor delivery
European Polymer J. 72: 484-93. -
Silva, L.A. et al. (2017) Collagen concentration on the facial skin of postmenopausal women after topical treatment with estradiol and genistein: a randomized double-blind controlled trial.
Gynecol Endocrinol. 33 (11): 845-8.
- RRID
- AB_620500
- UniProt
- P13941
- Entrez Gene
- Col3a1
- GO Terms
- GO:0001501 skeletal system development
- GO:0005586 collagen type III
View more products with COLLAGEN specificity
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