Collagen I antibody
Mutations in the encoding gene are associated with osteogenesis imperfecta (Sykes et al. 1990), Ehlers Danlos syndrome (Burrows et al. 1996), and idiopathic osteoporosis (Pernow et al. 2006).
Bio-Rad currently do not support the use Rabbit anti Rat Collagen I antibody in western Blotting. However, it has been reported that this antibody has been used successfully for the quantitation of collagen I in rabbit aortic tissue by western blotting (Valero-Muñoz et al. 2014).
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 500 µl of sterile DI water. Vial contains 0.05 mg of lyophilised antibody.
- Preparation
- Purified IgG prepared by affinity chromatography on immobilized rat collagen I
- Buffer Solution
- 0.1M Sodium Chloride
0.01M Sodium Phosphate
0.01M Sodium Borate - Preservative Stabilisers
- 1% Mannitol
1% Dextran - Immunogen
- Type I Collagen from rat tail tendon.
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P02454
- P02466
- Entrez Gene
- Col1a1
- Col1a2
- GO Terms
- GO:0005615 extracellular space
- GO:0009612 response to mechanical stimulus
- GO:0031960 response to corticosteroid stimulus
- GO:0042542 response to hydrogen peroxide
- GO:0043434 response to peptide hormone stimulus
- GO:0051591 response to cAMP
- GO:0071300 cellular response to retinoic acid
- GO:0071363 cellular response to growth factor stimulus
- GO:0005201 extracellular matrix structural constituent
- Regulatory
- For research purposes only
Applications of Collagen I antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/1500 | 1/6000 | |
Immunofluorescence | 1/10 | 1/60 | |
Immunohistology - Frozen | 1/10 | 1/60 | |
Immunohistology - Paraffin |
- Immunohistology
- It has been reported that this antibody is suitable for use on paraffin embedded formalin fixed sections following treatment with 1 mg/ml pepsin in 0.5 M acetic acid for 2 hours at 37ºC and multiple buffer washes.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Collagen I antibody
-
Daniel, C. et al. (2009) Thrombospondin-2 therapy ameliorates experimental glomerulonephritis via inhibition of cell proliferation, inflammation, and TGF-beta activation.
Am J Physiol Renal Physiol. 297: F1299-309. -
Hartner A, et al. (2006) Angiotensin II formation in the kidney and nephrosclerosis in Ren-2 hypertensive rats.
Nephrol Dial Transplant. 21:1778-85. -
Klanke B, et al. (2008) Blood pressure versus direct mineralocorticoid effects on kidney inflammation and fibrosis in DOCA-salt hypertension.
Nephrol Dial Transplant. 23: 3456-63. -
Jones, H.B. et al. (2012) Quantitative Histopathological Assessment of Retardation of Islets of Langerhans Degeneration in Rosiglitazone-dosed Obese ZDF Rats Using Combined Insulin and Collagens (I and III) Immunohistochemistry with Automated Image Analysis and Statistical Modeling.
Toxicol Pathol. 41: 425-44. -
Valero-Muñoz, M. et al. (2014) Carob pod insoluble fiber exerts anti-atherosclerotic effects in rabbits through sirtuin-1 and peroxisome proliferator-activated receptor-γ coactivator-1α.
J Nutr. 144: 1378-84. -
Aktaş, A. et al. (2013) Ankaferd-Induced Early Soft Tissue Wound Healing in an Experimental Rat Model.
Turkiye Klinikleri J Med Sci 2013;33(6): 1344-53. -
Oshita, T. et al. (2016) Adipose-Derived Stem Cells Improve Collagenase-Induced Tendinopathy in a Rat Model.
Am J Sports Med. 44 (8): 1983-9. -
Hartner, A. et al. (2016) Impaired Neovascularization and Reduced Capillary Supply in the Malignant vs. Non-malignant Course of Experimental Renovascular Hypertension.
Front Physiol. 7: 370. -
Martínez-Martínez E et al. (2016) The lysyl oxidase inhibitor (β-aminopropionitrile) reduces leptin profibrotic effects and ameliorates cardiovascular remodeling in diet-induced obesity in rats.
J Mol Cell Cardiol. 92: 96-104. -
Novotná, J. et al. (2001) Hyperoxia and recovery from hypoxia alter collagen in peripheral pulmonary arteries similarly.
Physiol Res. 50 (2): 153-63. -
Jones, H.B. et al. (2013) Quantitative histopathological assessment of retardation of islets of langerhans degeneration in rosiglitazone-dosed obese ZDF rats using combined insulin and collagens (I and III) immunohistochemistry with automated image analysis and statistical modeling.
Toxicol Pathol. 41 (3): 425-44. -
Dergilev, K.V. et al. (2017) Comparison of cardiac stem cell sheets detached by Versene solution and from thermoresponsive dishes reveals similar properties of constructs.
Tissue Cell. 49 (1): 64-71.
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