Collagen I antibody

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Rabbit anti Rat Collagen I

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2150-1908 C E IF P 0.5 ml
Rabbit anti Rat Collagen I antibody recognizes both native and heat denatured rat collagen type I. Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (~95 kDa) is found in bone, cornea, skin and tendon.

Mutations in the encoding gene are associated with osteogenesis imperfecta (Sykes et al. 1990), Ehlers Danlos syndrome (Burrows et al. 1996), and idiopathic osteoporosis (Pernow et al. 2006).

Bio-Rad currently do not support the use Rabbit anti Rat Collagen I antibody in western Blotting. However, it has been reported that this antibody has been used successfully for the quantitation of collagen I in rabbit aortic tissue by western blotting (Valero-Muñoz et al. 2014).

Product Details

Target Species
Rat
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 500 µl of sterile DI water. Vial contains 0.05 mg of lyophilised antibody.
Preparation
Purified IgG prepared by affinity chromatography on immobilized rat collagen I
Preservative Stabilisers
Contains mannitol, dextran & salts
Immunogen
Type I Collagen from rat tail tendon.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P02454 Related reagents
P02466 Related reagents
Entrez Gene
Col1a1 Related reagents
Col1a2 Related reagents
GO Terms
GO:0005615 extracellular space
GO:0009612 response to mechanical stimulus
GO:0031960 response to corticosteroid stimulus
GO:0042542 response to hydrogen peroxide
GO:0043434 response to peptide hormone stimulus
GO:0051591 response to cAMP
GO:0071300 cellular response to retinoic acid
GO:0071363 cellular response to growth factor stimulus
GO:0005201 extracellular matrix structural constituent
Regulatory
For research purposes only

Applications of Collagen I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1500 1/6000
Immunofluorescence 1/10 1/60
Immunohistology - Frozen 1/10 1/60
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Immunohistology
It has been reported that this antibody is suitable for use on paraffin embedded formalin fixed sections following treatment with 1 mg/ml pepsin in 0.5 M acetic acid for 2 hours at 37ºC and multiple buffer washes.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for Collagen I antibody

  1. Daniel, C. et al. (2009) Thrombospondin-2 therapy ameliorates experimental glomerulonephritis via inhibition of cell proliferation, inflammation, and TGF-beta activation.
    Am J Physiol Renal Physiol. 297: F1299-309.
  2. Hartner A, et al. (2006) Angiotensin II formation in the kidney and nephrosclerosis in Ren-2 hypertensive rats.
    Nephrol Dial Transplant. 21:1778-85.
  3. Klanke B, et al. (2008) Blood pressure versus direct mineralocorticoid effects on kidney inflammation and fibrosis in DOCA-salt hypertension.
    Nephrol Dial Transplant. 23: 3456-63.
  4. Jones, H.B. et al. (2012) Quantitative Histopathological Assessment of Retardation of Islets of Langerhans Degeneration in Rosiglitazone-dosed Obese ZDF Rats Using Combined Insulin and Collagens (I and III) Immunohistochemistry with Automated Image Analysis and Statistical Modeling.
    Toxicol Pathol. 41: 425-44.
  5. Valero-Muñoz, M. et al. (2014) Carob pod insoluble fiber exerts anti-atherosclerotic effects in rabbits through sirtuin-1 and peroxisome proliferator-activated receptor-γ coactivator-1α.
    J Nutr. 144: 1378-84.
  6. Aktaş, A. et al. (2013) Ankaferd-Induced Early Soft Tissue Wound Healing in an Experimental Rat Model.
    Turkiye Klinikleri J Med Sci 2013;33(6): 1344-53.
  7. Oshita, T. et al. (2016) Adipose-Derived Stem Cells Improve Collagenase-Induced Tendinopathy in a Rat Model.
    Am J Sports Med. 44 (8): 1983-9.
  8. Hartner, A. et al. (2016) Impaired Neovascularization and Reduced Capillary Supply in the Malignant vs. Non-malignant Course of Experimental Renovascular Hypertension.
    Front Physiol. 7: 370.
  9. Martínez-Martínez E et al. (2016) The lysyl oxidase inhibitor (β-aminopropionitrile) reduces leptin profibrotic effects and ameliorates cardiovascular remodeling in diet-induced obesity in rats.
    J Mol Cell Cardiol. 92: 96-104.
  10. Novotná, J. et al. (2001) Hyperoxia and recovery from hypoxia alter collagen in peripheral pulmonary arteries similarly.
    Physiol Res. 50 (2): 153-63.
  11. Jones, H.B. et al. (2013) Quantitative histopathological assessment of retardation of islets of langerhans degeneration in rosiglitazone-dosed obese ZDF rats using combined insulin and collagens (I and III) immunohistochemistry with automated image analysis and statistical modeling.
    Toxicol Pathol. 41 (3): 425-44.
  12. Dergilev, K.V. et al. (2017) Comparison of cardiac stem cell sheets detached by Versene solution and from thermoresponsive dishes reveals similar properties of constructs.
    Tissue Cell. 49 (1): 64-71.