Collagen I antibody
Rabbit anti Rat collagen I antibody used for demonstration of collagen I expression in rat kidney by immunohistochemistry on paraffin embedded tissue sections.
Image caption:
Renin, collagen and PCNA stainings in renal tissue.
Representative photomicrographs of renin, collagen I and PCNA staining in the kidneys of experimental groups. Sham=sham operated controls, NMH=non-malignant hypertension, MH=malignant hypertension, neg. control=IgG control staining for specificity of antibody.
From: Hartner A, Jagusch L, Cordasic N, Amann K, Veelken R, Jacobi J and Hilgers KF (2016)
Impaired Neovascularization and Reduced Capillary Supply in the Malignant vs. Non-malignant Course of Experimental Renovascular Hypertension.
Front. Physiol. 7:370.
Rabbit anti Rat Collagen I antibody recognizes both native and heat denatured rat collagen type I. Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (~95 kDa) is found in bone, cornea, skin and tendon.
Mutations in the encoding gene are associated with osteogenesis imperfecta (Sykes et al. 1990), Ehlers Danlos syndrome (Burrows et al. 1996), and idiopathic osteoporosis (Pernow et al. 2006).
Bio-Rad currently do not support the use Rabbit anti Rat Collagen I antibody in western Blotting. However, it has been reported that this antibody has been used successfully for the quantitation of collagen I in rabbit aortic tissue by western blotting (Valero-Muñoz et al. 2014).
Mutations in the encoding gene are associated with osteogenesis imperfecta (Sykes et al. 1990), Ehlers Danlos syndrome (Burrows et al. 1996), and idiopathic osteoporosis (Pernow et al. 2006).
Bio-Rad currently do not support the use Rabbit anti Rat Collagen I antibody in western Blotting. However, it has been reported that this antibody has been used successfully for the quantitation of collagen I in rabbit aortic tissue by western blotting (Valero-Muñoz et al. 2014).
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 500 µl of sterile DI water. Vial contains 0.05 mg of lyophilised antibody.
- Preparation
- Purified IgG prepared by affinity chromatography on immobilized rat collagen I
- Preservative Stabilisers
- Contains mannitol, dextran & salts
- Immunogen
- Type I Collagen from rat tail tendon.
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. - Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P02454 Related reagents
- P02466 Related reagents
- Entrez Gene
- Col1a1 Related reagents
- Col1a2 Related reagents
- GO Terms
- GO:0005615 extracellular space
- GO:0009612 response to mechanical stimulus
- GO:0031960 response to corticosteroid stimulus
- GO:0042542 response to hydrogen peroxide
- GO:0043434 response to peptide hormone stimulus
- GO:0051591 response to cAMP
- GO:0071300 cellular response to retinoic acid
- GO:0071363 cellular response to growth factor stimulus
- GO:0005201 extracellular matrix structural constituent
- Regulatory
- For research purposes only
Applications of Collagen I antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1500 | 1/6000 | |
| Immunofluorescence | 1/10 | 1/60 | |
| Immunohistology - Frozen | 1/10 | 1/60 | |
| Immunohistology - Paraffin |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Immunohistology
- It has been reported that this antibody is suitable for use on paraffin embedded formalin fixed sections following treatment with 1 mg/ml pepsin in 0.5 M acetic acid for 2 hours at 37ºC and multiple buffer washes.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
References for Collagen I antibody
-
Daniel, C. et al. (2009) Thrombospondin-2 therapy ameliorates experimental glomerulonephritis via inhibition of cell proliferation, inflammation, and TGF-beta activation.
Am J Physiol Renal Physiol. 297: F1299-309. -
Hartner A, et al. (2006) Angiotensin II formation in the kidney and nephrosclerosis in Ren-2 hypertensive rats.
Nephrol Dial Transplant. 21:1778-85. -
Klanke B, et al. (2008) Blood pressure versus direct mineralocorticoid effects on kidney inflammation and fibrosis in DOCA-salt hypertension.
Nephrol Dial Transplant. 23: 3456-63. -
Jones, H.B. et al. (2012) Quantitative Histopathological Assessment of Retardation of Islets of Langerhans Degeneration in Rosiglitazone-dosed Obese ZDF Rats Using Combined Insulin and Collagens (I and III) Immunohistochemistry with Automated Image Analysis and Statistical Modeling.
Toxicol Pathol. 41: 425-44. -
Valero-Muñoz, M. et al. (2014) Carob pod insoluble fiber exerts anti-atherosclerotic effects in rabbits through sirtuin-1 and peroxisome proliferator-activated receptor-γ coactivator-1α.
J Nutr. 144: 1378-84. -
Aktaş, A. et al. (2013) Ankaferd-Induced Early Soft Tissue Wound Healing in an Experimental Rat Model.
Turkiye Klinikleri J Med Sci 2013;33(6): 1344-53. -
Oshita, T. et al. (2016) Adipose-Derived Stem Cells Improve Collagenase-Induced Tendinopathy in a Rat Model.
Am J Sports Med. 44 (8): 1983-9. -
Hartner, A. et al. (2016) Impaired Neovascularization and Reduced Capillary Supply in the Malignant vs. Non-malignant Course of Experimental Renovascular Hypertension.
Front Physiol. 7: 370. -
Martínez-Martínez E et al. (2016) The lysyl oxidase inhibitor (β-aminopropionitrile) reduces leptin profibrotic effects and ameliorates cardiovascular remodeling in diet-induced obesity in rats.
J Mol Cell Cardiol. 92: 96-104. -
Novotná, J. et al. (2001) Hyperoxia and recovery from hypoxia alter collagen in peripheral pulmonary arteries similarly.
Physiol Res. 50 (2): 153-63. -
Jones, H.B. et al. (2013) Quantitative histopathological assessment of retardation of islets of langerhans degeneration in rosiglitazone-dosed obese ZDF rats using combined insulin and collagens (I and III) immunohistochemistry with automated image analysis and statistical modeling.
Toxicol Pathol. 41 (3): 425-44. -
Dergilev, K.V. et al. (2017) Comparison of cardiac stem cell sheets detached by Versene solution and from thermoresponsive dishes reveals similar properties of constructs.
Tissue Cell. 49 (1): 64-71.
