CNTF antibody
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Rabbit anti Rat CNTF
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- CNTF
Antibody Quality and Performance Guarantee
| Rabbit anti Rat CNTF antibody recognizes rat ciliary neurotrophic factor (CNTF).. . Rat CNTF is a 22.7 kDa neuroregulatory cytokine which is found primarily in peripheral nerve tissue. CNTF promotes the survival and differentiation of neurons and other neuronal cell types. |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1 ml distilled water.
The addition of 0.09% sodium azide is recommended for long term storage.
N.B. For functional studies do not add sodium azide. - Antiserum Preparation
- Antisera to rat CNTF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant rat CNTF.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
20ng/ml | 50ng/ml |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
||
| Western Blotting | |
0.1ug/ml | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with the appropriate negative/positive controls.
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References for CNTF antibody
-
Müller, A. et al. (2007) Astrocyte-derived CNTF switches mature RGCs to a regenerative state following inflammatory stimulation.
Brain. 130: 3308-20. -
Fischer, D. et al. (2008) Crystallins of the beta/gamma-superfamily mimic the effects of lens injury and promote axon regeneration.
Mol Cell Neurosci. 37: 471-9. -
Leibinger, M. et al. (2009) Neuroprotective and axon growth-promoting effects following inflammatory stimulation on mature retinal ganglion cells in mice depend on ciliary neurotrophic factor and leukemia inhibitory factor.
J Neurosci. 29: 14334-41. -
Hauk, T.G. et al. (2010) Stimulation of axon regeneration in the mature optic nerve by intravitreal application of the toll-like receptor 2 agonist Pam3Cys.
Invest Ophthalmol Vis Sci. 51: 459-64. -
Sengottuvel, V. et al. (2011) Taxol facilitates axon regeneration in the mature CNS.
J Neurosci. 31: 2688-99. -
Askvig, J.M. et al. (2012) Neuronal activity and axonal sprouting differentially regulate CNTF and CNTF receptor complex in the rat supraoptic nucleus.
Exp Neurol. 233 (1): 243-52.
- Synonyms
- Ciliary Neurotrophic Factor
- RRID
- AB_2276485
- UniProt
- P20294
- Entrez Gene
- Cntf
- GO Terms
- GO:0005829 cytosol
- GO:0005125 cytokine activity
- GO:0005127 ciliary neurotrophic factor receptor binding
- GO:0005634 nucleus
- GO:0040007 growth
- GO:0007259 JAK-STAT cascade
- GO:0008083 growth factor activity
- GO:0008284 positive regulation of cell proliferation
- GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
- View More GO Terms
- GO:0046887 positive regulation of hormone secretion
- GO:0030307 positive regulation of cell growth
- GO:0030424 axon
- GO:0032838 cell projection cytoplasm
- GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
- GO:0043025 neuronal cell body
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0043524 negative regulation of neuron apoptosis
- GO:0043526 neuroprotection
- GO:0048143 astrocyte activation
- GO:0048680 positive regulation of axon regeneration
- GO:0051291 protein heterooligomerization
- GO:0060075 regulation of resting membrane potential
- GO:0060081 membrane hyperpolarization
AAR21
158915
161303
162275
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