Rabbit anti Rat CNTF antibody recognizes rat ciliary neurotrophic factor (CNTF)..
Rat CNTF is a 22.7 kDa neuroregulatory cytokine which is found primarily in peripheral nerve tissue. CNTF promotes the survival and differentiation of neurons and other neuronal cell types.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 0.1 ml distilled water.
The addition of 0.09% sodium azide is recommended for long term storage.
N.B. For functional studies do not add sodium azide.
- Antiserum Preparation
- Antisera to rat CNTF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant rat CNTF.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of reconstitution.
- Entrez Gene
- GO Terms
ciliary neurotrophic factor receptor binding
growth factor activity
positive regulation of cell proliferation
positive regulation of phosphatidylinositol 3-kinase cascade
positive regulation of hormone secretion
positive regulation of cell growth
cell projection cytoplasm
positive regulation of tyrosine phosphorylation of Stat3 protein
neuronal cell body
positive regulation of MAPKKK cascade
negative regulation of neuron apoptosis
positive regulation of axon regeneration
regulation of resting membrane potential
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CNTF antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with the appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Product Specific References
References for CNTF antibody
Fischer, D. et al. (2008) Crystallins of the beta/gamma-superfamily mimic the effects of lens injury and promote axon regeneration.
Mol Cell Neurosci. 37: 471-9.
Leibinger, M. et al. (2009) Neuroprotective and axon growth-promoting effects following inflammatory stimulation on mature retinal ganglion cells in mice depend on ciliary neurotrophic factor and leukemia inhibitory factor.
J Neurosci. 29: 14334-41.
Sengottuvel, V. et al. (2011) Taxol facilitates axon regeneration in the mature CNS.
J Neurosci. 31: 2688-99.
Müller, A. et al. (2007) Astrocyte-derived CNTF switches mature RGCs to a regenerative state following inflammatory stimulation.
Brain. 130: 3308-20.
Hauk, T.G. et al. (2010) Stimulation of axon regeneration in the mature optic nerve by intravitreal application of the toll-like receptor 2 agonist Pam3Cys.
Invest Ophthalmol Vis Sci. 51: 459-64.
Askvig, J.M. et al. (2012) Neuronal activity and axonal sprouting differentially regulate CNTF and CNTF receptor complex in the rat supraoptic nucleus.
Exp Neurol. 233 (1): 243-52.