CNTF antibody

100% Secure

Rabbit anti Rat CNTF

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAR21 C E FN IF WB 0.1 mg
Rabbit anti Rat CNTF antibody recognizes rat ciliary neurotrophic factor (CNTF)..
.
Rat CNTF is a 22.7 kDa neuroregulatory cytokine which is found primarily in peripheral nerve tissue. CNTF promotes the survival and differentiation of neurons and other neuronal cell types.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1 ml distilled water.

The addition of 0.09% sodium azide is recommended for long term storage.

N.B. For functional studies do not add sodium azide.
Antiserum Preparation
Antisera to rat CNTF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant rat CNTF
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P20294 Related reagents
Entrez Gene
Cntf Related reagents
GO Terms
GO:0005829 cytosol
GO:0005125 cytokine activity
GO:0005127 ciliary neurotrophic factor receptor binding
GO:0005634 nucleus
GO:0040007 growth
GO:0007259 JAK-STAT cascade
GO:0008083 growth factor activity
GO:0008284 positive regulation of cell proliferation
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
GO:0046887 positive regulation of hormone secretion
GO:0030307 positive regulation of cell growth
GO:0030424 axon
GO:0032838 cell projection cytoplasm
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0043025 neuronal cell body
GO:0043410 positive regulation of MAPKKK cascade
GO:0043524 negative regulation of neuron apoptosis
GO:0043526 neuroprotection
GO:0048143 astrocyte activation
GO:0048680 positive regulation of axon regeneration
GO:0051291 protein heterooligomerization
GO:0060075 regulation of resting membrane potential
GO:0060081 membrane hyperpolarization
Regulatory
For research purposes only

Applications of CNTF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 20ng/ml 50ng/ml
Immunofluorescence
Immunohistology - Frozen
Western Blotting 0.1ug/ml 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with the appropriate negative/positive controls.
ELISA
This antibody may be used in an indirect ELISA with PRP26 as the standard.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Application Based External Images

Immunofluorescence

Product Specific References

References for CNTF antibody

  1. Fischer, D. et al. (2008) Crystallins of the beta/gamma-superfamily mimic the effects of lens injury and promote axon regeneration.
    Mol Cell Neurosci. 37: 471-9.
  2. Leibinger, M. et al. (2009) Neuroprotective and axon growth-promoting effects following inflammatory stimulation on mature retinal ganglion cells in mice depend on ciliary neurotrophic factor and leukemia inhibitory factor.
    J Neurosci. 29: 14334-41.
  3. Sengottuvel, V. et al. (2011) Taxol facilitates axon regeneration in the mature CNS.
    J Neurosci. 31: 2688-99.
  4. Müller, A. et al. (2007) Astrocyte-derived CNTF switches mature RGCs to a regenerative state following inflammatory stimulation.
    Brain. 130: 3308-20.
  5. Hauk, T.G. et al. (2010) Stimulation of axon regeneration in the mature optic nerve by intravitreal application of the toll-like receptor 2 agonist Pam3Cys.
    Invest Ophthalmol Vis Sci. 51: 459-64.
  6. Askvig, J.M. et al. (2012) Neuronal activity and axonal sprouting differentially regulate CNTF and CNTF receptor complex in the rat supraoptic nucleus.
    Exp Neurol. 233 (1): 243-52.