Albumin antibody
Sheep anti Rat albumin antibody (0220-2424) used to demonstrate albumin expression in murine brain by immunofluorescence.
Image caption:
Surfactant protein-G (SP-G) and vascular endothelia in striatal ischemic areas of mice with impaired vasculature’s integrity indicated by extravasation of endogenous albumin into the parenchyma. Representative triple fluorescence labeling of albumin (Alb), SP-G (red), and lectin-based visualization of the vasculature (LEA) in mice 72 h after focal cerebral ischemia (A–A’’’).
From: Michalski D, Reimann W, Spielvogel E, Mages B, Biedermann B, Barthel H, Nitzsche B, Schob S, Härtig W.
Regionally Altered Immunosignals of Surfactant Protein-G, Vascular and Non-Vascular Elements of the Neurovascular Unit after Experimental Focal Cerebral Ischemia in Mice, Rats, and Sheep.
Int J Mol Sci. 2022 May 24;23(11):5875.
doi: 10.3390/ijms23115875.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Sheep anti Rat albumin antibody (0220-2424) used to demonstrate albumin expression in murine brain by immunofluorescence.
Image caption:
Surfactant protein-G (SP-G) and vascular endothelia in striatal ischemic areas of mice with impaired vasculature’s integrity indicated by extravasation of endogenous albumin into the parenchyma. Representative triple fluorescence labeling of albumin (Alb), SP-G (red), and lectin-based visualization of the vasculature (LEA) in mice 72 h after focal cerebral ischemia (A–A’’’), added by a higher magnification of the same labeling at the ischemic border zone (B). Exemplary combined detection of Alb, SP-G, and the vasculature (LEA) at a higher magnification captured by laser-scanning microscopy
From: Michalski D, Reimann W, Spielvogel E, Mages B, Biedermann B, Barthel H, Nitzsche B, Schob S, Härtig W.
Regionally Altered Immunosignals of Surfactant Protein-G, Vascular and Non-Vascular Elements of the Neurovascular Unit after Experimental Focal Cerebral Ischemia in Mice, Rats, and Sheep.
Int J Mol Sci. 2022 May 24;23(11):5875.
doi: 10.3390/ijms23115875.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Sheep anti Rat albumin antibody (0220-2424) used to demonstrate albumin expression in murine brain by immunofluorescence.
Image caption:
Surfactant protein-G (SP-G) and vascular endothelia in striatal ischemic areas of mice with impaired vasculature’s integrity indicated by extravasation of endogenous albumin into the parenchyma. Representative triple fluorescence labeling of albumin (Alb), SP-G (red), and lectin-based visualization of the vasculature (LEA) in mice 72 h after focal cerebral ischemia (C–C’’). Triple fluorescence labeling of SP-G, fibronectin (FN), and the vasculature (LEA) after 72 h of focal cerebral ischemia.
From: Michalski D, Reimann W, Spielvogel E, Mages B, Biedermann B, Barthel H, Nitzsche B, Schob S, Härtig W.
Regionally Altered Immunosignals of Surfactant Protein-G, Vascular and Non-Vascular Elements of the Neurovascular Unit after Experimental Focal Cerebral Ischemia in Mice, Rats, and Sheep.
Int J Mol Sci. 2022 May 24;23(11):5875.
doi: 10.3390/ijms23115875.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
E IF ResetSheep anti Rat Albumin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Albumin
Antibody Quality and 
Unrivaled 
| Sheep anti Rat albumin antibody recognizes rat albumin, a soluble protein which constitutes approximately 50% of blood serum protein, acting as a regulator of blood colloidal osmotic pressure and hence blood volume. albumin is also an important transporter of substances within the bloodstream, such as fatty acids, thyroid hormones, metal ions and steroids. |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified Ig - liquid
- Preparation
- Purified Ig prepared by affinity chromatography on Protein G
- Antiserum Preparation
- Antiserum to rat albumin was raised by repeated immunisation of sheep with highly purified antigen.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Native albumin from rat serum
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/20 000 | 1/200 000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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References for Albumin antibody
-
Westin, J.E. et al. (2006) Endothelial proliferation and increased blood-brain barrier permeability in the basal ganglia in a rat model of 3,4-dihydroxyphenyl-L-alanine-induced dyskinesia.
J Neurosci. 26: 9448-61. -
Eyre J, et al. (2007) Statin-sensitive endocytosis of albumin by glomerular podocytes.
Am J Physiol Renal Physiol 292: F674-81. -
Alonso, A. et al. (2010) Reorganization of gap junctions after focused ultrasound blood-brain barrier opening in the rat brain.
J Cereb Blood Flow Metab. 30: 1394-402. -
Obeid, S. et al. (2016) Development of a NanoBioAnalytical platform for "on-chip" qualification and quantification of platelet-derived microparticles.
Biosens Bioelectron. pii: S0956-5663(16)30856-9. -
Michalski, D. et al. (2022) Regionally Altered Immunosignals of Surfactant Protein-G, Vascular and Non-Vascular Elements of the Neurovascular Unit after Experimental Focal Cerebral Ischemia in Mice, Rats, and Sheep.
Int J Mol Sci. 23 (11): 5875.
- RRID
- AB_2226081
- UniProt
- P02770
- Entrez Gene
- Alb
- GO Terms
- GO:0003677 DNA binding
- GO:0005504 fatty acid binding
- GO:0005604 basement membrane
- GO:0005615 extracellular space
- GO:0006810 transport
- GO:0007584 response to nutrient
- GO:0008144 drug binding
- GO:0008270 zinc ion binding
- GO:0009267 cellular response to starvation
- View More GO Terms
- GO:0010033 response to organic substance
- GO:0019836 hemolysis by symbiont of host erythrocytes
- GO:0015643 toxin binding
- GO:0019899 enzyme binding
- GO:0046689 response to mercury ion
- GO:0030170 pyridoxal phosphate binding
- GO:0042311 vasodilation
- GO:0043066 negative regulation of apoptosis
- GO:0043234 protein complex
- GO:0043498 cell surface binding
- GO:0046010 positive regulation of circadian sleep/wake cycle, non-REM sleep
- GO:0051659 maintenance of mitochondrion location
- GO:0070541 response to platinum ion
View more products with ALBUMIN specificity
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