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IgG IgM antibody

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Goat anti Rabbit IgG/IgM:Biotin (Human Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG IgM

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Goat anti Rabbit IgG/IgM antibody reacts with the heavy and light chains of rabbit IgG and IgM and with the light chains of rabbit IgA as demonstrated by ELISA and flow cytometry.

Target Species
Rabbit
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Antiserum Preparation
Antisera to rabbit IgG and IgM were raised by repeated immunizations of goats with normal rabbit IgG and IgM.
The Ig fraction was purified by affinity chromatography on pooled rabbit IgG and IgM covalently linked to agarose.
The preparation has been cross absorbed with pooled human sera and purified human paraproteins to remove any potential cross reactivity.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Normal rabbit IgG + IgM.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/20,000
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

How to Use the Spectraviewer

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  • Select combined or multi-laser view to visualize the spectra

References for IgG IgM antibody

  1. Iver, S. et al. (2019) Probing BAK and BAX Activation and Pore Assembly with Cytochrome c Release, Limited Proteolysis, and Oxidant-Induced Linkage.
    Methods Mol Biol. 1877: 201-16.
  2. Campos-Ordoñez, T. et al. (2021) Chronic exposure to cyclohexane induces stereotypic circling, hyperlocomotion, and anxiety-like behavior associated with atypical c-Fos expression in motor- and anxiety-related brain regions.
    Behav Brain Res. Nov 12;113664.

UniProt
P01780
P03988
P04221
GO Terms
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005886 plasma membrane
GO:0016021 integral to membrane

STAR4010B

156146 159776 165245 169207

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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