IgG antibody

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Goat anti Rabbit IgG (H/L):HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
STAR124P C E WB 1 mg
Goat anti Rabbit IgG antibody conjugated to horseradish peroxidase, recognizes rabbit IgG. Cross reactivity with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat and sheep serum proteins has been minimized by solid phase adsorption.

Product Details

Target Species
Rabbit
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
Reconstitution
Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antisera to rabbit IgG were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Gentamicin sulfate
1%Bovine Serum Albumin
Immunogen
Rabbit IgG, whole molecule.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

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GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/50000 1/100000
Immunohistology - Frozen 1/1000 1/5000
Western Blotting 1/2000 1/10000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for IgG antibody

  1. Fernandes, A.C. et al. (2014) Lab-on-chip cytometry based on magnetoresistive sensors for bacteria detection in milk.
    Sensors (Basel). 14 (8): 15496-524.