IgG antibody

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Goat anti Rabbit IgG (H/L):HRP

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: IgG
Region: (H/L)

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References (2)
Applications
Useful Reagents
Storage Information
Batch/Lot Specific Datasheets

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Goat anti Rabbit IgG antibody conjugated to horseradish peroxidase, recognizes rabbit IgG. Cross reactivity with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat and sheep serum proteins has been minimized by solid phase adsorption.

Target Species: Rabbit
Product Form: Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilized
Reconstitution: Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation: Antisera to rabbit IgG were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.01% Gentamicin sulfate
1% Bovine Serum Albumin
Immunogen: Rabbit IgG, whole molecule.
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml after reconstitution
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/50000	1/100000
Immunohistology - Frozen	1/1000	1/5000
Western Blotting	1/2000	1/10000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for IgG antibody

Fernandes, A.C. et al. (2014) Lab-on-chip cytometry based on magnetoresistive sensors for bacteria detection in milk.
Sensors (Basel). 14 (8): 15496-524.
Shea, G.K. et al. (2020) Juxtacrine signalling via Notch and ErbB receptors in the switch to fate commitment of bone marrow-derived Schwann cells.
Eur J Neurosci. 52 (5): 3306-21.